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Colloidal gold immunocolorimetry kit for detecting retinol conjugated protein (RBP) and preparation method of kit

A technology of colloidal gold and kits, applied in the field of in vitro diagnostic reagents, can solve problems such as large impact, and achieve high sensitivity, good repeatability, and high specificity

Inactive Publication Date: 2016-08-24
CHEMCLIN DIAGNOSTICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the past β 2 -M is a conventional indicator, but in recent years, with the in-depth research on the physical and chemical properties of small molecular proteins, it has been found that temperature and pH have no effect on β 2 -M has a greater impact

Method used

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  • Colloidal gold immunocolorimetry kit for detecting retinol conjugated protein (RBP) and preparation method of kit
  • Colloidal gold immunocolorimetry kit for detecting retinol conjugated protein (RBP) and preparation method of kit
  • Colloidal gold immunocolorimetry kit for detecting retinol conjugated protein (RBP) and preparation method of kit

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Embodiment 1

[0032] Preparation of RBP calibrator

[0033] Dissolve the recombinant human RBP protein (commercially available) in the calibrator diluent (bovine serum albumin 0.5%, sodium azide 0.1%, PBS buffer pH 8.0) to prepare calibrator with different concentration gradients, and use the nine-strong RBP latex to immunize The colorimetric kit detects and prepares calibrator, detects each concentration gradient 3 times, takes the average value of the detection results, and the fixed value is 0 mg / L (calibration product diluent, without adding RBP protein), 8.75 mg / L, 17.5 mg / L L, 35mg / L, 70mg / L, 140mg / L.

[0034] Preparation of Reaction Buffer Reagent (Reagent 1)

[0035] Take 80mL of 50mM PBS pH8.0 buffer and add PEG6000-200005g, polysucrose-4001g, bovine serum albumin 0.5g, sodium azide 0.1g, and finally use the corresponding buffer to dilute to 100mL, and use a 0.22um microporous Filter through a filter membrane to prepare a reaction buffer reagent (reagent 1), including PEG6000-200...

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Abstract

The invention provides a colloidal gold immunocolorimetry kit for detecting retinol conjugated protein (RBP) and a preparation method of the kit and belongs to the field of in-vitro diagnostic reagents. The kit is used for judging whether the situation of individual acute kidney injury occurs or not. According to the method, colloidal gold particles are adopted as a carrier, a retinol conjugated protein (RBP for short) antibody and the colloidal gold particles are coupled, RBP protein in a sample makes contact with the coupled gold particles in a corresponding detection device, an antigen-antibody-gold compound is formed, the light absorbance of the gold particles under detection wavelength is changed, the change amount of the light absorbance is positively correlated with the concentration of RBP protein, and then the purpose of detecting RBP protein in the sample is achieved. The kit has high analysis sensitivity.

Description

field of invention [0001] The invention belongs to the field of in vitro diagnostic reagents, in particular to a colloidal gold immunocolorimetric assay kit for detecting retinol-binding protein (RBP) technical background [0002] Retinol Binding Protcin (RBP) is a transporter of retinol (vitamin A) in blood. In 1961, Berggard discovered in immunoelectrophoresis in α 2 -Proteins whose globulin domains form a long precipitation line, formerly known as long alpha 2 -globulin. In the following decades, people conducted a comprehensive study on the molecular structure and biological characteristics of this protein, and found that this protein is widely distributed in normal human body fluids, and belongs to the α 1 - A globulin that has the function of transporting retinol from hepatocytes to surrounding tissues. It is now believed that RBP in the blood mainly exists in the form of complexes bound by retinol and prealbumin. When retinol in the complex binds to target cells, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
Inventor 郭成志王志新
Owner CHEMCLIN DIAGNOSTICS CO LTD
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