Method for reinforcing flocculation performance of rhodopseudomonas faecalis of light-fermentative bacteria

A Rhodopseudomonas, photo-fermentation technology, applied in microorganism-based methods, bacteria, fermentation and other directions, can solve the problem of low hydrogen production efficiency, biomass loss, and poor flocculation characteristics of photo-fermentative bacteria in photo-fermentation hydrogen-producing reactors. problem, to achieve the effect of flocculation growth, reduction of strain loss, and effective separation

Inactive Publication Date: 2012-12-12
HARBIN INST OF TECH
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Problems solved by technology

[0004] The present invention aims to solve the problems of poor flocculation performance of photofermentation bacteria and continuous loss of biomass, resulting in low hydrogen production efficiency of photofermentation hydrogen production reactors, and provides a method for enhancing the flocculation performance of photofermentation bacteria Rhodopseudomonas faecalis

Method used

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  • Method for reinforcing flocculation performance of rhodopseudomonas faecalis of light-fermentative bacteria
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  • Method for reinforcing flocculation performance of rhodopseudomonas faecalis of light-fermentative bacteria

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specific Embodiment approach 1

[0011] Embodiment 1: The method for enhancing the flocculation performance of photofermentation bacteria Rhodopseudomonas faecalis in this embodiment is carried out according to the following steps:

[0012] 1. Inoculate the Rhodopseudomonas faecalis (Rhodopseudomonas faecalis) RLD-53 bacterium solution with a cell concentration of 0.8 to 1.0 g / L into the growth medium at an inoculum size of 10%, then place it on a shaker at 120 rpm, and Cultivate in continuous light at 35°C for 16 hours, and the light intensity is 150W / m 2 , get the seed solution;

[0013] 2. Inoculate the seed solution obtained in step 1 into the hydrogen-producing medium containing 0.75-1.25g / L L-cysteine ​​according to the inoculum amount of 10%, then place it on a shaker at 120rpm, and place it under continuous light at 35°C After culturing for 72 hours, a bacterial suspension containing flocs is obtained.

[0014] Rhodopseudomonas faecalis (Rhodopseudomonas faecalis) RLD-53 described in step 1 of this ...

specific Embodiment approach 2

[0017] Specific embodiment two: the difference between this embodiment and specific embodiment one is: the growth medium described in step one is made of 1g sodium acetate, 1g sodium succinate, 1.0g ammonium chloride, 1g beef extract, 0.5g peptone, 0.2 g sodium chloride, 0.2g magnesium sulfate heptahydrate, 0.012g ferrous sulfate heptahydrate, 0.08g anhydrous calcium chloride, 1mL trace element solution, 0.5g dipotassium hydrogen phosphate, 0.5g potassium dihydrogen phosphate, 0.5g L - Cysteine, 1mL growth factor solution and 1L distilled water, pH 7.0; each liter of trace element solution consists of 0.05mg manganese chloride tetrahydrate, 0.05g copper sulfate pentahydrate, 5mg ferric chloride hexahydrate, 0.5mg Cobalt nitrate hexahydrate, 1mg zinc sulfate heptahydrate, 1mg boric acid and the rest of distilled water; each liter of growth factor solution consists of 0.35g nicotinic acid, 0.1g biotin, 0.3g thiamine hydrochloride, 0.1g calcium pantothenate, 0.2g Composed of p-am...

specific Embodiment approach 3

[0018] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is: the hydrogen production medium described in step two is composed of 4.1g sodium acetate, 1.69g sodium glutamate, 0.2g sodium chloride, 0.2g seven Magnesium sulfate hydrochloride, 0.012g ferrous sulfate heptahydrate, 0.08g anhydrous calcium chloride, 1mL trace element solution, 0.5g dipotassium hydrogen phosphate, 0.5g potassium dihydrogen phosphate, 1mL growth factor solution and 1L distilled water; One liter of trace element solution consists of 0.05mg manganese chloride tetrahydrate, 0.05g copper sulfate pentahydrate, 5mg ferric chloride hexahydrate, 0.5mg cobalt nitrate hexahydrate, 1mg zinc sulfate heptahydrate, 1mg boric acid and the rest of distilled water; Growth factor solution consists of 0.35g niacin, 0.1g biotin, 0.3g thiamine hydrochloride, 0.1g calcium pantothenate, 0.2g p-aminobenzoic acid, 0.05g vitamin B12, 0.1g pyridoxammonium hydrochloride and the re...

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Abstract

A method for reinforcing flocculation performance of rhodopseudomonas faecalis of light-fermentative bacteria relates to a method for reinforcing flocculation performance of rhodopseudomonas faecalis and aims to solve the problems that flocculation performance of existing light-fermentative cells is poor, and hydrogen generation of a light-fermentative hydrogen generation reactor is low in efficiency due to continuous loss of biomass live-weight. The method includes steps of firstly, seeding 10% of rhodopseudomonas faecalis RLD-53 bacterium liquid to a growth culture medium, disposing the growth culture medium on a swing bed of 120rpm, culturing in continuous light with illumination intensity of 150W/m2 at the temperature of 35 DEG C for 16 hours, and obtaining seed liquid; secondly, seeding the seed liquid obtained in the first step to a hydrogen generation culture medium containing L-cysteine according to 10% seeding quantity, disposing the hydrogen generation culture medium on the swing bed of 120rpm, and culturing in continuous light at the temperature of 35 DEG C for 72 hours, and obtaining bacteria suspension containing flocculating constituents. The method can effectively improve flocculation performance of light-fermentative cells and realize flocculation growth of the light-fermentative cells and can be used for the field of light-fermentative hydrogen generation.

Description

technical field [0001] The invention relates to a method for enhancing the flocculation performance of Rhodopseudomonas faecalis. Background technique [0002] The continuous reduction of fossil fuel reserves and the continuous growth of energy demand, as well as the environmental pollution and greenhouse gas emissions caused by the burning of fossil fuels, have brought great challenges to the energy supply and the earth's environment in the 21st century. Renewable clean energy will become the main body of the future sustainable energy system. Hydrogen is clean and renewable, and its combustion only produces water and huge energy. Biohydrogen production technology has mild reaction conditions, low energy consumption, and can properly solve the contradiction between energy and the environment. Biological hydrogen production by photofermentation is under anaerobic light conditions. Photofermentation bacteria use small molecular organic matter, reduced inorganic sulfide or hyd...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P3/00C12N1/20C12R1/01
Inventor 任南琪谢国俊刘冰峰丁杰任宏宇
Owner HARBIN INST OF TECH
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