Carrier attachment type cyclic culture device of heterotrophic nitrification-aerobic denitrification bacterial agent

Abstract

The invention discloses a carrier attachment type cyclic culture device of a heterotrophic nitrification-aerobic denitrification bacterial agent. The carrier attachment type cyclic culture device comprises a main reactor and a pre-reactor, wherein a material supplement port and a culture medium injection port are formed at the top of each reactor, an aeration device, a sedimentation area and a discharge valve are arranged at the lower part of each reactor, the reactors are respectively connected with two solid-liquid separators through the discharge valves; and the main reactor is communicated with the solid-liquid separator of the pre-reactor through the material supplement port. The pre-reactor utilizes a residual solution after culture of the main reactor for re-culture and an unsaturated carrier is further transferred into the main reactor for further culture. The carrier attachment type cyclic culture device disclosed by the invention can fully utilize a culture medium solution, provide a pre-attachment process for the carrier, improve the utilization rate of a culture medium and the attachment efficiency of the carrier, avoid the excessive loss of the bacterial agent, improve the stability and the tolerance of the bacterial agent and realize the self-control continuous operation of the two reactors.

Description

technical field [0001] The invention belongs to environmental engineering water treatment microbial bacterial agent cultivation, in particular to a carrier-attached circulation culture device for heterotrophic nitrification-aerobic denitrification bacterial agent. Background technique [0002] Circulation culture is a culture method in which cells are retained in a culture tank in some way for recycling, usually by sedimentation, centrifugation and membrane filtration. Each of these methods has some advantages and disadvantages. The sedimentation method has simple equipment and low energy consumption. However, due to the dense medium, especially in the later stage of cultivation, the organism and the medium are mixed together in a jelly-like shape, which is difficult to settle and the culture waste is difficult to separate. ;The centrifugation method is suitable for industrial culture media containing various particles, and is more suitable for large-scale systems, but the p...

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Problems solved by technology

Each of these methods has some advantages and disadvantages. The sedimentation method has simple equipment and low energy consumption. However, due to the dense medium, especially in the later stage of cultivation, the organism and the medium are mixed together in a jelly-like shape, which is difficult to settle and the culture waste is difficult to separate. ;The centrifugation method is suitable for industrial culture media containing various particles, and is more suitable for large-scale systems, but the process is more complicated and the energy consumption is higher; the membrane filtration method is more thorough in removing waste liquid, but the membrane is easily blocked, and pumps need to be added, etc. equipment, high energy consumption

These cyclic culture methods not only have some defects in the cultivation stage, but also have some problems in the preservation stage of the inoculum. Because the water content of the inoculum is high, and it is not easy to use methods such as air drying to remove moisture, the dry storage of the inoculum is more difficult.

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