Method for culturing Clostridium butyricum with ultra-high cell concentration

A technology of Clostridium butyricum and cell concentration, applied in the biological field, can solve the problems that the number of bacteria cannot be guaranteed and the production cost is high, and achieve the effect of increasing the concentration and improving the quality.

Inactive Publication Date: 2012-01-25
SHENYANG JIANZHU UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, using the traditional strain production process, the number of strains cannot be guaranteed, and the production cost is high

Method used

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  • Method for culturing Clostridium butyricum with ultra-high cell concentration
  • Method for culturing Clostridium butyricum with ultra-high cell concentration

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Embodiment Construction

[0018] First prepare the basal medium of Clostridium butyricum T4, which consists of (g / L): glucose 10, yeast powder 2, peptone 2, NaCl 4, MgSO 4 ·7H 2 O 0.1, FeSO 4 ·7H 2 O 0.1, L-cysteine ​​0.5, trace element solution [MnSO 4 ·7H 2 O 0.01, CaCl 2 2H 2 O 0.01, ZnSO 4 ·7H 2 O 0.05, NaMoO 4 0.01,H 3 BO 3 0.01, CoCl 2 ·6H 2 O 0.2, A1K (SO 4 ) 2 0.01] 10 mL, vitamin solution [ascorbic acid 0.025, cobalamin 0.01, folic acid 0.01, creatine 0.025] 10 mL, resazurin (0.1%) 1 mL, and the initial pH was adjusted to 7.2. The prepared Clostridium butyricum T4 medium was put into a 500 mL Erlenmeyer flask with a stopper and sterilized at 124°C for 20 min. After the medium was cooled to room temperature, buffers of different concentrations were added through a 0.45 μm filter membrane to The blank without buffer was used as a control to investigate the effects of different types of buffer on the growth of Clostridium butyricum T4. The experiment was carried out in a shaker...

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Abstract

The invention provides a method for culturing Clostridium butyricum T4 with ultra-high cell concentration. The method is characterized by comprising the following steps of: promoting the Clostridium butyricum T4 to grow in the conventional Clostridium butyricum T4 culture medium by using urea as physiologically alkaline salt; and controlling the concentration of the urea in the liquid culture medium to be within the range of between 1.0 and 3.0g / L. The method has the advantages that: the inhibition effect produced on the growth of the Clostridium butyricum T4 due to the overquick reduction of the pH value of the culture medium can be relieved to a certain degree; meanwhile, the sustained growth of the Clostridium butyricum T4 can also be promoted so as to finally achieve the higher cell concentration. The industrialized production of a Clostridium butyricum T4 liquid with ultra-high cell concentration is promoted.

Description

Technical field [0001] The present invention is a biotechnology field, especially providing a method for cultivating ultra -high cell concentrations, which involves a carbon -containing organic composition composition that promotes the continuous growth of butyl -borne. Background technique [0002] Tannacus, also known as cases, is a specifically anaerobbal bacteria, which has multiple effects such as regulating the composition of human or animal gastrointestinal flora, prevention and treatment of various diarrhea, promotion of growth, and regulating immunity.In recent years, the research on butyl bacteria at home and abroad is mainly concentrated in medicine, and the research on feed has gradually heated up. [0003] The activity of T -T -Tutoobacterium is closely related to its number. To promote its application, the key is to solve the problem of high -density culture of the bacteria.During the growth process of butyramoids produce a large amount of organic acid metabolites, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/145
Inventor 王兴祖
Owner SHENYANG JIANZHU UNIVERSITY
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