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Method for fermenting and producing coenzyme Q10

A technology of coenzyme and aerobic fermentation, applied in the field of fermentation engineering, can solve the problems of high production cost and achieve the effects of low production cost, simple process and high bacterial concentration

Active Publication Date: 2010-05-12
ANHUI BBCA FERMENTATION TECH ENG RES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] At present, many strains have been used to produce CoQ10 by fermentation, but they have not been produced on a large scale due to reasons such as high production costs.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Agrobacterium tumefaciens ATCC2182 was cultured in shake flasks, and then inoculated into the seed medium with the following composition at an inoculum size of 10%: glucose 1%, peptone 1.5%, yeast extract 0.5%, sodium chloride 0.2%, pH6. 5. 50ml / 500ml Erlenmeyer flask, 250 rpm, temperature 30°C, culture for 18h.

[0026] Agrobacterium tumefaciens ATCC2182 was inoculated into a fermentation medium with the following composition at an inoculum size of 10%: glucose 20%, ammonium sulfate 1.5%, potassium dihydrogen phosphate 0.5%, magnesium sulfate 0.05%, corn steep liquor 2.5%, phosphoric acid Diammonium hydrogen 1%, pH6.5±0.2. Then fermentation was carried out under the following control conditions: temperature 30° C., ventilation ratio 1:0.5, stirring speed 500 rpm, pH control at 6.5, and fermentation for 72 hours.

[0027] Take the fermentation broth at 5000 rpm, centrifuge for 15 minutes to collect the bacteria, resuspend the collected bacteria with 10% NaOH methanol s...

Embodiment 2

[0029] Agrobacterium tumefaciens ATCC2337 is carried out shaking flask culture, then inoculates in the seed culture medium that has following composition: glucose 3%, peptone 0.5%, yeast extract 1.5%, sodium chloride 1.0%, pH7.5, culture condition 50ml / 500ml Erlenmeyer flask, 250 rpm, temperature 30°C, cultured for 20h.

[0030] Agrobacterium tumefaciens ATCC2337 was inoculated at an inoculum size of 10% into a medium with the following composition: glucose 18%, ammonium sulfate 1.2%, potassium dihydrogen phosphate 0.4%, magnesium sulfate 0.04%, corn steep liquor 2%, hydrogen phosphate Diammonium 0.8%, pH6.6±0.2. Then fermentation was carried out under the following control conditions: temperature 30° C., ventilation ratio 1:0.4, stirring speed 600 rpm, pH control 6.5, and fermentation for 60 hours.

[0031] Take the fermentation broth at 5000 rpm, centrifuge for 15 minutes to collect the bacteria, resuspend the collected bacteria with 10% NaOH methanol solution, transfer to...

Embodiment 3

[0033] Agrobacterium tumefaciens ATCC4452 is carried out shaking flask culture, then inoculates in the seed culture medium that has following composition: glucose 2%, peptone 1.0%, yeast extract 1.5%, sodium chloride 0.8%, pH7.0, culture condition 50ml / 500ml Erlenmeyer flask, 250 rpm, temperature 28°C, cultured for 16h.

[0034] Inoculate Agrobacterium tumefaciens ATCC4452 at an inoculum size of 15% into a medium with the following composition: glucose 15%, ammonium sulfate 0.8%, potassium dihydrogen phosphate 0.3%, magnesium sulfate 0.03%, corn steep liquor 1.5%, hydrogen phosphate Diammonium 0.5%, pH6.6±0.2. Then fermentation was carried out under the following control conditions: temperature 30° C., ventilation ratio 1:0.3, stirring speed 300 rpm, pH control at 7.5, and fermentation for 80 hours.

[0035] Take the fermentation broth at 5000 rpm, centrifuge for 10 minutes to collect the bacteria, resuspend the collected bacteria with 8% NaOH methanol solution, transfer to ...

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Abstract

The invention provides a method for fermenting and producing coenzyme Q10, comprising the following steps: firstly carrying out high-density aerobic fermentation for 60 to 84 hours on agrobacterium tumefaciens by inoculum size of 5 to 15 percent; then carrying out separation on the obtained fermentation liquor and obtaining separated thallus; and finally suspending the separated thallus, then backflowing, extracting and obtaining the coenzyme Q10, wherein the fermentation culture medium comprises the following components by weight percent: 12 to 20 percent of glucose, 0.5 to 1.5 percent of ammonium sulfate, 0.1 to 0.5 percent of monopotassium phosphate, 0.01 to 0.05 percent of magnesium sulfate, 0.5 to 2.5 percent of corn steep liquor and 0.2 to 1 percent of diammonium hydrogen phosphate; and pH is 6 to 7.5 and the fermentation temperature is 28 to 31 DEG C. The method has simple technique, low thallus concentration of the fermentation liquor and low production cost and is applicable to large-scale production.

Description

technical field [0001] The invention belongs to the field of fermentation engineering and relates to a method for producing coenzyme Q10 (CoQ10) by fermentation. Background technique [0002] CoQ10, also known as ubiquinone, is a fat-soluble quinone compound that widely exists in organisms. As a cell metabolism and cell respiration activator, it is also an important antioxidant and non-specific immune enhancer. It has anti-myocardial ischemia, anti-heart failure, Antiarrhythmic effect. The nutrition that can activate human cells and cell energy can improve human immunity, enhance anti-oxidation, delay aging and enhance human vitality. It is one of the indispensable important factors for human health. [0003] At present, the main production methods of CoQ10 are: animal and plant extraction, plant cell culture, chemical synthesis and microbial fermentation. [0004] The animal and plant tissue extraction method has disadvantages such as high raw material prices, limited sou...

Claims

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Application Information

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IPC IPC(8): C12P7/66C12R1/01
Inventor 李荣杰薛培俭尚海涛郑辉
Owner ANHUI BBCA FERMENTATION TECH ENG RES
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