Growth promoting agent for promoting photosynthetic bacteria strain to quickly breed and using method of growth promoting agent

A technology for photosynthetic bacteria and growth-promoting agent, applied in the field of microorganisms, can solve problems such as the establishment of a dominant population of photosynthetic bacteria, the inability to meet production applications, and the small number of bacterial cells, achieve large water purification or accumulate effective substances, and promote photosynthetic bacteria. Growth, the effect of promoting rapid reproduction

Inactive Publication Date: 2013-08-14
HEBEI WANLIN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, an excessively high pH value will inhibit the growth of photosynthetic bacteria itself, resulting in a small number of final bacterial cells; and in the process of cultivating photosynthetic bacteria with industrial and agricultural wastewater as raw material (medium), since most of the water is slightly acidic, Consuming a large amount of lye to increase the pH value is not only costly, but also pollutes the environment. In such a slightly

Method used

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  • Growth promoting agent for promoting photosynthetic bacteria strain to quickly breed and using method of growth promoting agent
  • Growth promoting agent for promoting photosynthetic bacteria strain to quickly breed and using method of growth promoting agent
  • Growth promoting agent for promoting photosynthetic bacteria strain to quickly breed and using method of growth promoting agent

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0029] Example 1

[0030] Follow the steps below:

[0031] (1) Activation of photosynthetic bacteria (indoor):

[0032] Prepare the seed fermentation medium commonly used in the photosynthetic bacteria laboratory, put it into a 150ml transparent glass anaerobic bottle, and autoclave at 115°C for 30 minutes. After cooling, perform strict aseptic operation and insert the purchased Rhodopeudomonas palustris (Rhodopeudomonas palustris strain preservation number CGMCC1.2180) strain, and carry out continuous light culture under 2500 lux of incandescent light for 3 to 5 days at a culture temperature of 28 -32℃, the activated photosynthetic bacteria strains are prepared. Check the photosynthetic bacteria under a microscope for robustness, lively movement and no pollution.

[0033] The composition of the commonly used seed fermentation medium in the laboratory is as follows:

[0034] Ammonium chloride 1.0g, sodium acetate 1.0g, magnesium sulfate 0.2g, sodium bicarbonate 1.0g, sodium chloride ...

Example Embodiment

[0049] Example 2

[0050] The present invention can be operated according to the following steps:

[0051] (1) Activation of photosynthetic bacteria: same as in Example 1(1);

[0052] (2) The method is the same as in Example 1(2).

[0053] The difference is that the composition of the added photosynthetic bacteria growth promoter is shown in Table 3 of the design plan.

[0054] Add different concentrations of ethanol (see the design plan table 3 for the added amount), set up a total of 7 treatments, numbered 1, 2, 3, 4, 5, 6, 7 (control).

[0055] table 3

[0056] Numbering

[0057] Test results: as shown in Table 4.

[0058] The test results show that adding 0.05mmol / L or 0.08mmol / L mixture of sorbic acid, potassium sorbate, sodium sorbate and ethanol to the commonly used seed culture medium in the laboratory can promote the growth of photosynthetic bacteria and increase photosynthetic bacteria. number.

[0059] Table 4

[0060] Numbering

Example Embodiment

[0061] Example 3

[0062] The present invention can be operated according to the following steps:

[0063] (1) Activation of photosynthetic bacteria: same as in Example 1(1);

[0064] (2) The effects of photosynthetic bacteria growth promoters cultured under different pH conditions on growth (under laboratory conditions): the operation method is the same as that of Example 1(2);

[0065] The difference is that the composition of the photosynthetic bacteria growth promoter added to the commonly used seed culture in the laboratory is 0.05mmol / L sorbic acid and 0.2mmol / L ethanol, and the pH value is adjusted to 6.5, 7.0, 7.5, 8.0, respectively. .

[0066] Test results: The culture results of different pH value culture conditions are shown in Table 5.

[0067] table 5

[0068] pH value

[0069] The test results show that the pH value is in the range of 6.5 to 8.0, and the addition of 0.05 mmol / L sorbic acid and 9 mmol / L ethanol can promote the growth of photosynthetic bacteria to varying ...

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Abstract

The invention relates to a growth promoting agent for promoting photosynthetic bacteria strain to quickly breed and a using method of the growth promoting agent. The growth prompting agent is obtained as follows: adding 1 part weight of one or a mixture of sorbic acid, potassium sorbate or sodium sorbate to 1-4 parts by weight of alcohol for stirring, mixing and dissolving. The using method of the growth promoting agent is as follows: adding the growth promoting agent to a prepared photosynthetic bacteria liquid medium for stirring until the growth promoting agent is sufficiently dissolved; adding active photosynthetic bacteria strains for filling to a sealed or semi-open light transmission container for culturing at 25-32 DEG C by radiating under 1500lux-1600lux, wherein sorbate radial concentration of the growth promoting agent, which is added to the photosynthetic bacteria liquid medium, is controlled within a range of 0.005 mmol/L-1.0 mmol/L. The growth promoting agent for promoting photosynthetic bacteria strain to quickly breed is simple to use, less in usage, low in cost and capable of promoting photosynthetic bacteria strain to quickly breed. Moreover, the using method of the growth promoting agent is safe, ecological and environment-friendly and extensive in market prospect.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a growth-promoting agent for promoting the rapid propagation of photosynthetic bacteria strains and a use method thereof. Background technique [0002] Photosynthetic bacteria (Photosynthetic Bacteria referred to as PSB) ecological distribution is very wide, mainly distributed in oceans, rivers, lakes, activated sludge and soil, is a kind of aquatic and hydrosphere microorganisms. Photosynthetic bacteria use sunlight as energy source, sodium acetate, sodium lactate, mannitol, malic acid, citric acid, glucose, glycerin and other organic substances as carbon and nitrogen sources, with fast growth and reproduction speed, wide source of medium, metabolite nutrition rich features. Photosynthetic bacterial cells are rich in protein, amino acid, biologically essential vitamins, antiviral active factors, coenzyme Q10 and a variety of physiologically active substances, which...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/01
Inventor 张根伟张闻笛王子晴
Owner HEBEI WANLIN BIOTECH
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