Growth promoting agent for promoting photosynthetic bacteria strain to quickly breed and using method of growth promoting agent
A technology for photosynthetic bacteria and growth-promoting agent, applied in the field of microorganisms, can solve problems such as the establishment of a dominant population of photosynthetic bacteria, the inability to meet production applications, and the small number of bacterial cells, achieve large water purification or accumulate effective substances, and promote photosynthetic bacteria. Growth, the effect of promoting rapid reproduction
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Embodiment 1
[0030] Follow these steps:
[0031] (1) Activation of photosynthetic bacteria (indoor):
[0032] Prepare a seed fermentation medium commonly used in the photosynthetic bacteria laboratory, put it into a transparent glass anaerobic bottle with a capacity of 150ml, and sterilize it under high pressure at 115°C for 30 minutes. After cooling, perform strict aseptic operation, insert the purchased Rhodopeudomonas palustris strains (CGMCC1.2180) strains, and carry out continuous light culture under incandescent light at 2500 lux for 3 to 5 days at a temperature of 28 -32°C, the activated photosynthetic bacteria strains were obtained. Check under the microscope that the photosynthetic bacteria are robust, active and free of pollution.
[0033] The composition of the commonly used seed fermentation medium in the laboratory is as follows:
[0034] Ammonium chloride 1.0g, sodium acetate 1.0g, magnesium sulfate 0.2g, sodium bicarbonate 1.0g, sodium chloride 0.5g, dipotassium hydrogen ...
Embodiment 2
[0050] The present invention can be operated according to the following steps:
[0051] (1) photosynthetic bacteria strain activation: with embodiment 1 (1);
[0052] (2) method is the same as embodiment 1 (2).
[0053] The difference is that the composition of the added photosynthetic bacteria growth promoter is shown in Table 3 of the design scheme,
[0054] Add different concentrations of ethanol (see the design scheme Table 3 for the amount added), and set up 7 treatments in total, numbered 1, 2, 3, 4, 5, 6, 7 (control).
[0055] table 3
[0056] serial number
1
2
3
4
5
6
7
Sorbic acid (mmol / L)
0.05
0.08
0
0
0
0
0
Potassium sorbate (mmol / L)
0
0
0.05
0.08
0
0
0
Sodium sorbate (mmol / L)
0
0
0
0
0.05
0.08
0
Ethanol (mmol / L)
0.15
0.20
0.15
0.20
0.15
0.20
0
[0057] Test results: as shown in Ta...
Embodiment 3
[0062] The present invention can be operated according to the following steps:
[0063] (1) photosynthetic bacteria strain activation: with embodiment 1 (1);
[0064] (2) Effect of photosynthetic bacteria growth-promoting agent on growth under different pH conditions (under laboratory conditions): operation method is the same as embodiment 1 (2);
[0065] The difference is that the photosynthetic bacteria growth promoter added to the commonly used seed medium in the laboratory consists of 0.05mmol / L sorbic acid and 0.2mmol / L ethanol, and the pH values are adjusted to 6.5, 7.0, 7.5, 8.0 respectively .
[0066] Test results: Table 5 shows the culture results of culture conditions with different pH values.
[0067] table 5
[0068] pH value
[0069] The test results showed that within the pH range of 6.5-8.0, adding 0.05mmol / L sorbic acid and 9mmol / L ethanol could promote the growth of photosynthetic bacteria to varying degrees, and after 4 days of cultivation, the...
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