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Method for producing L-lactic acid by using vinasse

A technology of liquor and waste residues, which is applied in the field of advanced processing and fermentation of agricultural and sideline products to prepare L-lactic acid, which can solve the problems of high cost of liquor waste and reuse, low efficiency of enzymatic hydrolysis of liquor, etc., to increase energy consumption and improve selection adaptability , the effect of reducing particle size

Active Publication Date: 2021-11-09
LUZHOU LAOJIAO CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is the low efficiency of enzymatic hydrolysis of liquor discarded grains in the prior art, and the high cost of reuse of liquor discarded grains

Method used

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  • Method for producing L-lactic acid by using vinasse
  • Method for producing L-lactic acid by using vinasse

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Experimental program
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Effect test

preparation example Construction

[0049] The preparation method of above-mentioned mixed bacteria enzyme liquid comprises the steps:

[0050] a. Sampling was taken from pit mud, waste grains, and surrounding soil of the winery, and was screened and enriched with a screening medium using liquor waste grains as a carbon source. After separation and purification, bacterial samples were obtained; after the bacterial samples were activated, use The solid fermentation medium is fermented and cultured, and the inoculation amount of the activated bacteria sample is 9-11% of the solid fermentation medium, and then it is extracted, filtered, and centrifuged to obtain the bacterial enzyme liquid A, and the bacterial enzyme liquid A is used for liquor Enzymatic hydrolysis of discarded grains, screening to obtain H bacteria, and fermenting H bacteria enzyme liquid, the enzyme activity of H bacteria enzyme liquid is 5.0-5.8IU / ml;

[0051] b. Sampling from the pit mud, waste grains, and surrounding soil of the winery, screen...

Embodiment 1

[0088] A. Mix the distilled grains with tap water at a solid-to-liquid ratio of 1:3 g / ml, soak for 4 hours, wet-grind the soaked grains to 200 mesh, and filter to obtain crushed grains;

[0089] B. Mix PEG-8000 with the mixed bacterial enzyme solution (H bacterial enzyme solution: L bacterial enzyme solution: M bacterial enzyme solution is 16:3:1) in a ratio of 0.004:1g / ml to obtain a mixed solution;

[0090] C. Mix the crushed and discarded grains obtained in step A with the mixed solution obtained in step B according to the ratio of 1:10g / ml, oscillate at 50°C and 140rpm for 72 hours for enzymolysis, and filter to obtain the initial sugar solution, Evaporate the initial sugar solution under reduced pressure to a reducing sugar concentration of 60g / L to obtain a concentrated sugar solution;

[0091] D. Use CaO to adjust the pH of the concentrated sugar solution to 6.5, then add yeast powder 0.6%, peptone 1.0%, K 2 HPO 4 0.2%, CaCO 3 6% mixed and then sterilized at 100°C to...

Embodiment 2

[0094] A. Mix the distilled grains with tap water at a solid-to-liquid ratio of 1:3 g / ml, soak for 4 hours, wet-grind the soaked grains to 250 mesh, and filter to obtain crushed grains;

[0095] B. Mix PEG-8000 with the mixed bacterial enzyme solution (H bacterial enzyme solution: L bacterial enzyme solution: M bacterial enzyme solution is 15:4:1) in a ratio of 0.005:1g / ml to obtain a mixed solution;

[0096] C. Mix the crushed and discarded grains obtained in step A with the mixed solution obtained in step B according to the ratio of 1:10g / ml, oscillate at 50°C and 140rpm for 72 hours for enzymolysis, and filter to obtain the initial sugar solution. Evaporate the initial sugar solution under reduced pressure to a reducing sugar concentration of 65g / L to obtain a concentrated sugar solution;

[0097] D. Use CaO to adjust the pH of the concentrated sugar solution to 6.5, then add yeast powder 0.6%, peptone 1.0%, K 2 HPO 4 0.2%, CaCO 36% mixed and then sterilized at 100°C to ...

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Abstract

The invention discloses a method for producing L-lactic acid by using vinasse, and belongs to the field of preparation of L-lactic acid by deep processing and fermentation of agricultural and sideline products. The method for producing the L-lactic acid by using the vinasse comprises the following steps that the vinasse is infiltrated; superfine grinding is carried out; a mixed enzyme solution is prepared; polyethylene glycol is added; enzymolysis is carried out on the vinasse; a diluted sugar solution is concentrated; the L-lactic acid is fermented and the like. The invention provides a more efficient method for performing enzymolysis utilization on the vinasse. By adopting superfine grinding of the vinasse, introduction of pretreatment chemical substances can be reduced on the premise of ensuring relatively high yield of the reducing sugar subjected to enzymolysis, so that the process cost is reduced; the adopted mixed bacterium enzyme liquid has relatively high stability, and the matched use has a remarkable mutual assistance effect on enzymolysis; and by adopting the method, 0.56-0.58 g of reducing sugar and 0.48-0.49 g of L-lactic acid can be produced from each g of the dry waste vinasse. The process is good in stability and relatively low in cost, and can effectively solve the problems of low efficiency of enzymolysis of the vinasse and relatively high cost of reutilization of the vinasse in the prior art.

Description

technical field [0001] The invention belongs to the field of L-lactic acid preparation through advanced processing and fermentation of agricultural and sideline products, and relates to a method for producing L-lactic acid by using distilled grains. Background technique [0002] Distillation waste is the main by-product of solid-state liquor brewing. According to the solid-state liquor production process, about 3 tons of liquor will be produced for every ton of liquor produced. With upgrading and transformation, the output of distillery waste will increase year by year. If such a huge amount of distillery waste is directly discharged into the environment without reasonable treatment, it will not only cause environmental pollution, but also cause a waste of resources. Distiller's grains contain 65-70% of total carbohydrates including starch, cellulose and hemicellulose, which can be treated to degrade polysaccharides and serve as a good carbon source for microbial fermentati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P19/02C12P7/56C12N1/20C12N1/14C12N1/02C12R1/685C12R1/785C12R1/885C12R1/07
CPCC12P39/00C12P19/02C12P7/56C12N1/20C12N1/14C12N1/02
Inventor 雷翔云邓波熊燕飞李勇敖灵丁海龙彭远松马卓宋川
Owner LUZHOU LAOJIAO CO LTD
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