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Preparation method of high-activity lactobacillus paracasei N1115 ready-to-eat probiotic powder

A technology of para-cheese and Lactobacillus, which is applied in the food field, can solve the problems of not providing a preparation method, and achieve the effects of increasing the yield, increasing the concentration of bacteria, and reducing blood sugar

Inactive Publication Date: 2018-06-05
JUNLEBAO DAIRY GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Chinese invention patent with the patent number 201110357058.8 involves "Lactobacillus paracasei N1115, its immunomodulatory effect and application", and mentions that Lactobacillus paracasei can be used to make bacterial powder, but does not provide a specific preparation method

Method used

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  • Preparation method of high-activity lactobacillus paracasei N1115 ready-to-eat probiotic powder
  • Preparation method of high-activity lactobacillus paracasei N1115 ready-to-eat probiotic powder
  • Preparation method of high-activity lactobacillus paracasei N1115 ready-to-eat probiotic powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-6

[0036] Embodiment 1-6 The preparation method of highly active Lactobacillus paracasei N1115 ready-to-eat probiotic powder

[0037] Embodiment 1-6 is respectively a kind of preparation method of highly active Lactobacillus paracasei N1115 ready-to-eat probiotic powder, and they carry out according to the following steps:

[0038] (1) Bacteria activation and proliferation culture

[0039] Inoculate Lactobacillus paracasei N1115 into the MRS liquid medium for activation;

[0040] The activated bacteria were inoculated in high-density medium (containing 20g / L glucose, 20g / L yeast powder, 0.09mol / L buffer salt, 50mg / L MnSO4•5H2O) for proliferation and culture, and the number of viable bacteria was detected , until the number of live bacteria reaches 106-108CFU / ml, the bacterial solution A is obtained;

[0041] (2) Collect bacteria

[0042] Centrifuge the bacterial solution A at a low temperature and high speed at 4°C and 12000 rpm, discard the supernatant to obtain the bacterial...

Embodiment 7

[0054] Example 7 The comparative test of the beneficial effects of using high-density medium in the preparation method of the highly active Lactobacillus paracasei N1115 instant probiotic powder of Examples 1-6

[0055] In this embodiment, by designing a comparative test, the use of a high-density culture medium during the activation and proliferation of the strains has been studied on the beneficial effects that can be produced on the number of live bacteria in the prepared bacterial powder. The specific test results are as follows:

[0056]

[0057] It can be seen that the use of high-density medium in the activation and proliferation of strains can increase the concentration of bacteria in the sludge, thereby increasing the yield of bacteria powder.

Embodiment 8

[0058] Example 8 Detection test on the number of viable bacteria within the shelf life of the highly active Lactobacillus paracasei N1115 ready-to-eat probiotic powder produced in Examples 1-6

[0059] In this embodiment, the number of live bacteria of various high-activity Lactobacillus paracasei N1115 ready-to-eat probiotic powders prepared in Examples 1-6 was tracked and detected within 18 months. The test results refer to figure 1 , show that the highly active Lactobacillus paracasei N1115 ready-to-eat probiotic powder provided by the present invention is within the shelf life of 18 months, and the number of live bacteria is gradually declining, but still remains at 10 when 18 months expire. 8 More than cfu / g.

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Abstract

The invention discloses a preparation method of high-activity lactobacillus paracasei N1115 ready-to-eat probiotic powder. The preparation method comprises four steps of sequentially performing strainactivation, performing proliferation culturing, collecting thalli, performing freeze drying and performing milling to obtain powder. The high-activity lactobacillus paracasei N1115 ready-to-eat probiotic powder provided by the invention can also maintain the state that the viable count is not less than 108CFU / g at the later stage of the quality guarantee period of 18 months, and the mouth feel isgood. The preparation method is used for preparing the ready-to-eat probiotic powder, and after the ready-to-eat probiotic powder is taken, the blood sugar can be reduced.

Description

technical field [0001] The invention belongs to the field of food, and relates to an instant probiotic powder, in particular to a preparation method of a highly active Lactobacillus paracasei N1115 instant probiotic powder. Background technique [0002] Probiotics are living microorganisms that, when ingested in certain amounts, can have an effect on the health of the host. At present, probiotics are mostly used in dairy products, including fermented milk, lactic acid bacteria drinks, side cheese, milk powder and other products, as well as biscuits, candies, vegetable juices, etc. In recent decades, Japan, the United States, Europe and other developed countries have developed The concept of probiotics has long been deeply rooted in the hearts of the people. At present, the proportion of functional fermented lactic acid bacteria milk drinks in Japan and Europe has reached 80% in the dairy market, and about 30% in North America. In recent years, in order to prevent bacteria ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/135C12N1/20C12R1/225
CPCA23L33/135C12N1/20A23V2400/125
Inventor 魏立华薛玉玲刘茁荀一萍冯丽莉张栋朱宏柴艳兵王世杰
Owner JUNLEBAO DAIRY GRP CO LTD
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