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Industrialized culture method for high-protein desert algae

A cultivation method and high-protein technology, applied in the direction of single-cell algae, etc., can solve the problems of low yield of desert green algae, unstable separation and cultivation method, etc.

Active Publication Date: 2014-01-29
新疆拓必达科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention provides a method for industrialized cultivation of high-protein desert green algae, which overcomes the above-mentioned deficiencies in the prior art, and can effectively solve the problem of existing desert green algae. Cultivation method The problem of unstable separation and cultivation method and low yield of cultivating desert green algae

Method used

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  • Industrialized culture method for high-protein desert algae
  • Industrialized culture method for high-protein desert algae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1, the industrialized cultivation method of this high-protein desert green algae is carried out according to the following steps: the first step, separates, and takes desert soil sample, adds the TAP liquid culture medium of 200ml to 400ml in every 50g to 100g desert soil sample Add TAP liquid medium to the desert soil sample, and shake it with a shaker for 5 min to 15 min at a speed of 100 rpm to 120 rpm to fully mix the sample with the medium, and place the mixture at a temperature of 25°C to 31°C under light Cultivate under the condition of 2000Lux to 3000Lux for 3 days to 15 days. After the culture supernatant turns green, apply every 200μL to 300μL supernatant on 25ml to 30ml TAP solid medium, and draw it under sterile conditions The supernatant is spread on a petri dish containing TAP solid medium, and cultured at a temperature of 25°C to 31°C and a light of 2000Lux to 3000Lux for 3 days to 15 days to obtain a culture of desert green algae;

[0021] ...

Embodiment 2

[0023] Embodiment 2, the industrialized cultivation method of this high-protein desert green algae is carried out according to the following steps: the first step, separates, and takes desert soil sample, adds the TAP liquid culture medium of 200ml or 400ml in every 50g or 100g desert soil sample Add TAP liquid medium to the desert soil sample, and shake it with a shaker for 5 min or 15 min at a speed of 100rpm or 120rpm to fully mix the sample with the medium, and place the mixture at 25°C or 31°C under light Cultivate for 3 days or 15 days under the condition of 2000Lux or 3000Lux, wait for the culture supernatant to turn green, apply every 200μL or 300μL of supernatant to 25ml or 30ml of TAP solid medium, and draw under sterile conditions The supernatant is spread on a petri dish containing TAP solid medium, and cultured at a temperature of 25°C or 31°C and a light of 2000Lux or 3000Lux for 3 days or 15 days to obtain a culture of desert green algae;

[0024] In the sec...

Embodiment 3

[0026] Embodiment 3, preferably as above-mentioned embodiment, TAP liquid culture medium is 0.4gNH 4 Cl, 0.156gMgSO 4 , 0.05gCaCl 2 ·H 2 O, 0.142gK 2 HPO·3H 2 O, 2.42g Tris Base, 1.5ml HCl, 1.0ml glacial acetic acid, 2g CH 3 COONa·3H 2 O and 1 ml of trace elements are dissolved in water to prepare 1 L of solution to obtain a TAP liquid medium, and the pH range of the TAP liquid medium is 6.8 to 7.0.

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Abstract

The invention relates to a culture method of microbe algae and in particular relates to an industrialized culture method for high-protein desert algae. The method comprises the following steps: 1, separating a desert alga culture; 2, inoculating a single desert alga colony to a fixed plate, and repeatedly culturing until eight or ten generations are inoculated to obtain the algae; 3, inoculating the algae into a liquid culture medium, and performing industrialized enclosed culture for 3-15 days. The desert algae are cultured at first, the cell concentration in the desert soil culture solution is improved, a target alga desert soil culture solution with high target alga biomass is obtained, the target algae are separated and purified to obtain high-yield target algae, and finally large-scale industrialized culture is performed, so that the target algae can be successfully separated. Moreover, the adopted culture method is totally sterile culture, pollution caused by pollution sources and harmful bacteria is avoided, and the desert algae can be cultured in a large scale under the condition that the yield of the desert algae is guaranteed.

Description

technical field [0001] The invention relates to a method for cultivating microbial algae, which is an industrialized method for cultivating high-protein desert green algae. Background technique [0002] Algae (Algae) is a class of autotrophic phytophytes with photosynthetic pigments in the plant kingdom, and has neither leaves, seeds or roots, nor flowers. Some algae are extremely small, only a few microns, but some are very large, very complex tissues, like broad streamers, such as kelp. Algae reproduce with spores in various shapes and sizes, most of which are primitive plants more than 2 billion years ago. Most algae have existed since ancient times and still exist today. Algae are so adaptable that they can survive even in extremely harsh climatic conditions, such as in salty or icy seas. [0003] As we all know, protein feed refers to beans, cakes, fishmeal, etc. whose natural moisture content is lower than 45%, crude fiber in dry matter is lower than 18%, and c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12
Inventor 艾山江阿不都克力木白克尔古丽山买买提
Owner 新疆拓必达科技发展有限公司
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