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Method for producing dejection degradation bacterium preparation

A production method and bacterial agent technology, applied in the field of feces-degrading bacterial agents, can solve the problems of large domestic sewage, large consumption of flushing water, etc., and achieve the effects of relatively low water content, convenient use, and low production cost

Inactive Publication Date: 2008-07-09
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] When the traditional water flushing toilet is used, it not only needs to consume a large amount of flushing water, but also produces a large amount of domestic sewage

Method used

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  • Method for producing dejection degradation bacterium preparation
  • Method for producing dejection degradation bacterium preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Bacillus cereus (Bacillus cereus, provided by the Microbiology Laboratory of Zhejiang University of Technology) and Bacillus licheniformis (Bacillus licheniformis, provided by the Microbiology Laboratory of Zhejiang University of Technology) were respectively made into a single strain inoculum by the following method:

[0025] The culture medium of the present embodiment is prepared according to the following dosage:

[0026] Beef extract peptone medium: 5g beef extract, 10g peptone, 5g sodium chloride, 20g agar, 1000ml water, pH7.2~7.4.

[0027] Liquid seed medium: 5g beef extract, 10g peptone, 5g sodium chloride, 1000ml water, pH7.2~7.4;

[0028] Solid medium: 400g bran, 600g water, mix well.

[0029] Take the strains of the above-mentioned feces-degrading bacteria on the slant of the beef extract peptone medium test tube, culture at 32-38°C for 18-24 hours to obtain slant seeds, and inoculate one ring of the slant seeds into a 250mL Erlenmeyer flask containing 100mL...

Embodiment 2

[0031] Respectively Staphylococcus xylosus (Staphylococcus xylosus, provided by Zhejiang University of Technology Microbiology Laboratory), Corynebacterium ammoniagenes (Ammoniagenic Corynebacterium, provided by Zhejiang University of Technology Microbiology Laboratory) and Carnobacterium mobil (active meat bacteria, provided by Zhejiang University of Technology Microbiology Laboratory ) is made into single-species inoculant by the following method:

[0032] The culture medium of the present embodiment is prepared according to the following dosage:

[0033] Beef extract peptone medium: 5g beef extract, 10g peptone, 5g sodium chloride, 20g agar, 1000ml water, pH7.2~7.4.

[0034] Liquid seed medium: 5g beef extract, 10g peptone, 5g sodium chloride, 1000ml water, pH7.2~7.4;

[0035] Solid medium: 360g bran, 40g corn flour, 600g water, mix well.

[0036]Take the strains of the above-mentioned feces-degrading bacteria on the slant of the beef extract peptone medium test tube, cul...

Embodiment 3

[0038] Bacteria use effect:

[0039] In order to clarify the use effect of the bacterial agent produced by the method of the present invention, get five kinds of single bacterial agent equal amounts that embodiment 1~2 makes and mix, then with the ratio of 0.03% (mass) and filler (by sawdust, peanut shell and Rice husks, etc.) are mixed, prepared as bio-filler, and put into a water-free degradable ecological toilet with a scale of 30 person-times; Ecological toilets were used as a control. Samples were taken regularly during use, and the chemical oxygen demand (COD), total nitrogen, total phosphorus, and total ash content of the biofiller eluent were detected respectively to investigate the feces degradation effect of the two. The results are shown in Figure 1~ 4. (the biofiller eluent is that 10g of biofillers add water and disperse in the fruit juicer for 10 minutes, replenish water and settle to 1 liter of supernatant; chemical oxygen demand (COD) detection adopts potassi...

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Abstract

The invention relates to a method for preparing fecal degradation bacterial agent, which comprises the following steps: inoculating fecal degradation bacteria on the beef extract peptone culture medium culture medium bevel; culturing under the temperature of 32-38 DEG C for 18-24 h to obtain the bevel, inoculating the bevel seed in the liquid seed culture medium, oscillating, aerating and culturing under the temperature of 32-38 DEG C and the rotary speed at 120-200 r / min for 18-24 h to obtain the liquid seed, inoculating the liquid seed in the solid culture medium whose water content is 50-70% under the inoculation quantity of 1-10%, blending evenly, aerating and culturing statically under the temperature at 32-37 DEG C for 48-72 h, finishing culturing when the cell density reaches to 10 <6>-10 <9> g, obtaining the solid culture, drying the solid culture or drying under the temperature at 20-60 DEG C, grinding the dried solid culture into the fecal degradation bacterial agent. The bacterial agent of the invention is very simple in preparation, low in cost, long in preservation and convenient in use.

Description

technical field [0001] The invention relates to a production method of a feces-degrading bacterial agent for a water-free degradable ecological toilet. Background technique [0002] When the traditional water flushing toilet is used, it not only needs to consume a large amount of flushing water, but also produces a large amount of domestic sewage. Today, with the increasing population of the world, rapid urbanization and economic development, shortage of water resources and a sharp increase in pollutants, the problems of large water consumption and high pollutant production in traditional toilets have become increasingly prominent. [0003] Therefore, many countries in the world have successively carried out research on water saving, water-free and emission reduction of toilets, especially public toilets, resulting in various types of new toilets, and water-free degradable ecological toilets are one of them. In the water-free degradable ecological toilet, directly below the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/44C12R1/085C12R1/10C12R1/01
Inventor 朱家荣吕伯升潘理黎单宁
Owner ZHEJIANG UNIV OF TECH
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