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Process of culturing photosynthetic bacteria with ultrahigh cell density

A technology of photosynthetic bacteria and cell concentration, applied in the direction of bacteria, etc., can solve the problems of maintaining at about 3 billion/ml, rising, etc., and achieve the effect of increasing the concentration of photosynthetic bacteria, alleviating inhibition, and improving quality

Inactive Publication Date: 2005-04-27
UNIV OF SCI & TECH BEIJING
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Problems solved by technology

Although sodium acetate can be used as a good carbon source for the growth of photosynthetic bacteria and can support the rapid growth of photosynthetic bacteria, as photosynthetic bacteria use sodium acetate, the pH of the culture will inevitably rise sharply, and too high pH will obviously inhibit the growth of photosynthetic bacteria. The continuous growth of photosynthetic bacteria leads to the concentration of photosynthetic bacteria cells produced by Chinese enterprises is generally only maintained at about 3 billion / ml, which is an important reason for limiting the rapid and sustainable growth of photosynthetic bacteria

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  • Process of culturing photosynthetic bacteria with ultrahigh cell density
  • Process of culturing photosynthetic bacteria with ultrahigh cell density

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[0015] First prepare the basal medium of photosynthetic bacteria, its composition is (g / L): NH 4 Cl 1.0, KH 2 PO 4 0.5, MgSO 4 7H 2 O 0.2, NaCl 2.0, yeast powder 0.5, trace element solution 1.0ml / L. Trace element solution includes (mg / L): FeCl 3 6H 2 O 5.0, CuSO 4 5H 2 O 0.05, H 3 BO 3 1.0, MnCl 2 4H 2 O 0.05, ZnSO 4 7H 2 O 1.0, CoCl 2 6H 2 O 0.5. After preparing trace element solution and photosynthetic bacteria culture medium with deionized water respectively, put the prepared photosynthetic bacteria culture medium into a 500ml glass bottle and carry out high-temperature and high-pressure sterilization at 124°C for 20 minutes. Add 1.9ml of absolute ethanol under aseptic conditions on the workbench to make the ethanol concentration reach 3.0g / L. At the same time, add 3.0g / L sodium acetate in the same medium without adding ethanol as a control. The research determines that sodium acetate and ethanol are respectively used as Effects of major organic carb...

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Abstract

The present invention provides one method of culturing photosynthetic bacteria with ultrahigh cell density. The present invention features that in the photosynthetic bacteria culturing medium, ethanol is used as the unique organic carbon source and the ethanol concentration in the liquid culture medium is controlled in 1.0-10.0 g / L. The present invention has the advantage of making photosynthetic bacteria grow continuously to reach high cell density while remitting the suppression of raised pH value on the growth of photosynthetic bacteria.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular provides a method for cultivating photosynthetic bacteria with ultra-high cell concentration, and relates to a carbon-containing organic component in a photosynthetic bacteria culture medium that supports the stable growth of photosynthetic bacteria. Background technique [0002] Photosynthetic bacteria (Photosynthetic Bacteria) is a general term for a large class of prokaryotic organisms that can perform non-oxygenous photosynthesis under anaerobic light conditions. ), Chlorobiaceae (Chlorobiaceae) and green filamentous fungi (Chloroflexaceae) (Chloroflexaceae) 4 families, bacterial cells are mainly divided into rod-shaped and spherical two different forms, is one of the pioneer species of the origin of life on earth. Among them, Rhodopseudomonas and Rhodospirillaceae are more photosynthetic bacteria species in industrial production. The protein content of photosynthetic bacteria ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20
Inventor 闫海何红胜魏玉霞周洁弓爱君吕乐
Owner UNIV OF SCI & TECH BEIJING
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