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Preparation method and application of micrococcus luteus immobilized beads

A technology for immobilizing small balls and luteus bacteria, which is applied in the field of microbial degradation of pollutants, can solve the problems of poor spherical shape, low mechanical strength, and easy breakage of embedded small balls, so as to improve the effect, improve mechanical strength, and promote growth. Effect

Active Publication Date: 2017-05-24
ZHEJIANG NORMAL UNIVERSITY
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  • Abstract
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Problems solved by technology

This method of immobilizing bacteria can purify and maintain high-efficiency strains, so it has the advantages of high processing efficiency, easy control of the reaction, high-purity and high-efficiency strains, and high biological concentration. Now commonly used embedding materials include polyvinyl alcohol (PVA), Sodium alginate (SA), but the embedding beads fixed by these materials generally have problems such as poor spherical shape, low mechanical strength, and easy breakage.

Method used

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  • Preparation method and application of micrococcus luteus immobilized beads

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Embodiment 2

[0034] 1. Preparation of the suspension of Bacteria luteus

[0035] Micrococcus Luteus IAM14879 can be purchased from the RIKEN Microorganism Collection. The Micrococcus Luteus IAM14879 preserved at -70°C was inoculated into the liquid revival medium for culture. The liquid revival medium consisted of: 3g of peptone, 3g of yeast extract, 1.5g of mineral salt solution, pH 7.3 ; Cultivate at 30°C for 36 hours to obtain the activated seed culture solution;

[0036] The obtained seed liquid is inoculated into the LMM liquid medium, wherein the composition of the LMM liquid medium is: 3.5g / L of NH 4 Cl, 1.5g / L of KH 2 PO 4 , 0.005g / L biotin, 0.02g / L L-methionine, 0.04g / L vitamin B1, 1.0g / L inosine, 0.03g / L MgSO 4 , 9.0g / L L-lithium lactate, 2.0ml / L mineral salt solution (0.375g / L CuSO 4 ·5H 2 O, 0.785g / L MnCl 2 4H 2 O, 0.18g / L FeSO 4 ·7H 2 O, 0.029g / L Na 2 MoO 4 2H 2 O, 0.089g / L ZnSO 4 ·7H 2 O, the solvent is water), the pH is 7.5, 160r / min shake culture 40h, obtain ...

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Abstract

The invention discloses a preparation method of micrococcus luteus immobilized beads. The method comprises the following steps: burying micrococcus luteus into a sodium alginate-porous soil filtering material mixed solution, so as to stably keep the activity of the micrococcus luteus; enabling the micrococcus luteus to grow into a source for providing resuscitation-promoting factor Rpf proteins for a long period, wherein the Rpf proteins can resuscitate and promote a potential functional bacterial group in a toxic and difficult-to-degrade organic pollution environment, so as to enhance efficient degradation of pollutants. The invention further provides application of the micrococcus luteus immobilized beads in the toxic organic pollutants; compared with common immobilized carrier powder active carbon, the operation cost is lower, and the micrococcus luteus immobilized beads have more remarkable economic benefits and environment-friendly benefits. The micrococcus luteus immobilized beads can be used for a biological treatment system of toxic and harmful, difficult-to-degrade and high-concentration organic industrial wastewater including coking wastewater, high-salt phenol-containing wastewater, printing and drying wastewater, pharmaceutical wastewater and the like.

Description

technical field [0001] The invention relates to the field of microbial degradation of pollutants, in particular to a method for preparing immobilized balls of coccus luteus and its application in degrading toxic organic pollutants. Background technique [0002] In recent years, with the rapid development of industries such as petrochemical, pharmaceutical, building materials, pesticides, papermaking, electronics, gas, textiles, dyes, plastics and coking, the discharge of refractory organic industrial wastewater has increased significantly. Industrial wastewater with refractory organic pollutants not only causes serious pollution to the environment, but also increasingly harms the human body, aquatic organisms and crops, seriously destroying the ecological balance. The treatment of toxic, harmful, refractory and high-concentration organic industrial wastewater has always been a research hotspot and difficulty in the field of sewage treatment at home and abroad. Therefore, ef...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N11/10C12N11/04C02F3/34C12R1/265
CPCC02F3/34C12N11/04C12N11/10C12N11/14
Inventor 苏晓梅张硕张云鸽丁林贤林红军陈建荣
Owner ZHEJIANG NORMAL UNIVERSITY
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