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A poplar explant detoxification reagent, detoxification method and application

A technology of explants and new poplars, applied in the field of forest tree genetics and breeding, can solve cumbersome problems and achieve the effects of simplifying detoxification steps, increasing elimination, and maintaining

Active Publication Date: 2022-05-27
NORTHEAST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a kind of poplar explant detoxification reagent, detoxification method and application, to solve the problems in the above-mentioned prior art, change the defective that conventional poplar explant detoxification method process is loaded down with trivial details, this method It is simple and easy to reduce the damage of explants to plant tissue caused by experimental operations and disinfectants, and can greatly improve the degerming efficiency of explants

Method used

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  • A poplar explant detoxification reagent, detoxification method and application
  • A poplar explant detoxification reagent, detoxification method and application
  • A poplar explant detoxification reagent, detoxification method and application

Examples

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Effect test

Embodiment 1

[0027] Embodiment 1 Poplar explant detoxification method

[0028] (1) Obtaining explants: The robust stems of Populus shanxin 30 days after cutting were selected as the source of explants, the surface of the explants was sprayed with 75% ethanol, the leaves and petioles were cut off, and one axillary bud was retained.

[0029] (2) Soaking: After the explants were obtained, they were soaked in a solution containing 100 mg / L cephalosporin, and the soaking time was 10 min.

[0030] (3) Rinse: Rinse the immersed explants twice with a solution containing 100 mg / L cephalosporin, and dry the surface water with sterile filter paper or absorbent paper.

[0031] (4) Disinfection: soak in 75% ethanol and sterile water containing 100mg / L cephalosporin for 15 minutes in turn, dry the surface water with sterile filter paper or absorbent paper, cut off the 1mm edge stem segments at both ends (cut off two The stem section after the end is 2.5 cm) to complete the explant disinfection treatmen...

Embodiment 2

[0035] Embodiment 2 Poplar explant detoxification method

[0036] (1) Obtaining explants: The robust stems of Populus shanxin at 35 days after cutting were selected as the source of explants, the surface of the explants was sprayed with 75% ethanol, the leaves and petioles were cut off, and two axillary buds were retained.

[0037] (2) Soaking: After the explants were obtained, they were soaked in a solution containing 150 mg / L cephalosporin, and the soaking time was 12 min.

[0038] (3) Rinse: Rinse the explants after soaking twice with a solution containing 150 mg / L cephalosporin, and dry the surface water with sterile filter paper or absorbent paper.

[0039] (4) Disinfection: soak in 75% ethanol and sterile water containing 150mg / L cephalosporin for 18min in turn, dry the surface moisture with sterile filter paper or absorbent paper, cut off the 1.5mm edge stem segments at both ends (cut off The stem segment at both ends is 2.5 cm) to complete the explant disinfection tre...

Embodiment 3

[0043] Embodiment 3 Poplar explant detoxification method

[0044] (1) Obtaining explants: The robust stems of Populus shanxin 40 days after cutting were selected as the source of explants, the surface of the explants was sprayed with 75% ethanol, the leaves and petioles were cut off, and two axillary buds were retained.

[0045] (2) Soaking: After the explants were obtained, they were soaked in a solution containing 200 mg / L cephalosporin, and the soaking time was 15 min.

[0046](3) Rinse: Rinse the soaked explants twice with a solution containing 200 mg / L cephalosporin, and dry the surface water with sterile filter paper or absorbent paper.

[0047] (4) Disinfection: soak in 75% ethanol and sterile water containing 200mg / L cephalosporin for 20min in turn, dry the surface water with sterile filter paper or absorbent paper, cut off the 1.5mm edge stem segments at both ends (cut off The stem segment at both ends is 3 cm) to complete the explant disinfection treatment.

[0048...

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Abstract

The invention discloses a poplar explant detoxification agent, a detoxification method and an application thereof, belonging to the technical field of tree genetics and breeding. The detoxification reagent includes: 75% ethanol and 100-200 mg / L cephalosporin solution, and the poplar explants are treated alternately with the two reagents. It also specifically discloses a specific method for detoxifying poplar explants by using the detoxification reagent. Through this method, only 75% ethanol and cephalosporin can be used to complete the detoxification treatment, which is simple and effective. Solve the problem that it is difficult to remove parasitic bacteria on the surface of poplar cutting seedlings, and complete the detoxification of a large number of poplar explants more quickly, saving time and manpower. In addition, the explants that have been detoxified by this method have strong rooting performance , can provide new working methods and breeding materials for poplar vegetative propagation and tissue culture.

Description

technical field [0001] The invention relates to the technical field of forest tree genetics and breeding, and relates to a poplar explant detoxification reagent, a detoxification method and application. Background technique [0002] The Shanxin poplar variety is a polyploid poplar with stable characteristics of the offspring obtained after more than 20 years of cross-combination with aspen as the female parent and Xinjiang poplar as the male parent. The trunk is straight, the bark is smooth and pale green, covered with white powder, and the fruit sequence falls off naturally without flying. Compared with the control variety Xinjiang poplar, this variety has stronger adaptability, better stress resistance, low temperature resistance, and no frost damage occurs when planted in most areas of northern China. The rooting ability of Shanxin poplar is poor, the survival rate of conventional cuttings is low, and various bacteria and fungi are parasitized on the surface and inside o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 陈肃于越刘智杰
Owner NORTHEAST FORESTRY UNIVERSITY
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