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Poplar explant detoxification reagent, detoxification method and application

A technology of explants and poplars, applied in the field of forest tree genetics and breeding, can solve cumbersome problems, achieve the effects of simplifying detoxification steps, saving time and manpower, and improving work efficiency

Active Publication Date: 2021-06-11
NORTHEAST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a kind of poplar explant detoxification reagent, detoxification method and application, to solve the problems in the above-mentioned prior art, change the defective that conventional poplar explant detoxification method process is loaded down with trivial details, this method It is simple and easy to reduce the damage of explants to plant tissue caused by experimental operations and disinfectants, and can greatly improve the degerming efficiency of explants

Method used

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  • Poplar explant detoxification reagent, detoxification method and application

Examples

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Effect test

Embodiment 1

[0027] Embodiment 1 poplar explant detoxification method

[0028] (1) Obtaining explants: Select the robust stems of Populus sinensis 30 days after cutting as the source of explants, spray the surface of the explants with 75% ethanol, cut off the leaves and petioles, and keep one axillary bud.

[0029] (2) Soaking: After the explants were obtained, they were soaked in a solution containing 100 mg / L cephalosporin for 10 minutes.

[0030] (3) Rinse: Rinse the soaked explants twice with a solution containing 100 mg / L cephalosporin, and dry the surface moisture with sterile filter paper or absorbent paper.

[0031] (4) Disinfection: Soak with 75% ethanol and sterile water containing 100mg / L cephalosporin for 15 minutes in turn, blot the surface moisture with sterile filter paper or absorbent paper, cut off the 1mm edge stem sections at both ends (cut off two The stem segment after the end is 2.5cm) to complete the explant disinfection treatment.

[0032] (5) Rooting culture: the...

Embodiment 2

[0035] Embodiment 2 poplar explant detoxification method

[0036] (1) Obtaining explants: Select the healthy stems of Populus sinensis 35 days after cutting as the source of explants, spray the surface of the explants with 75% ethanol, cut off the leaves and petioles, and keep 2 axillary buds.

[0037] (2) Soaking: After the explants were obtained, they were soaked in a solution containing 150 mg / L cephalosporin for 12 minutes.

[0038] (3) Rinse: Rinse the soaked explants twice with a solution containing 150 mg / L cephalosporin, and dry the surface moisture with sterile filter paper or absorbent paper.

[0039] (4) Disinfection: Soak in 75% ethanol and sterile water containing 150mg / L cephalosporin for 18 minutes in turn, blot the surface moisture with sterile filter paper or absorbent paper, and cut off the 1.5mm edge stem sections at both ends (cut off The rear stem section at both ends is 2.5cm) to complete the explant disinfection treatment.

[0040] (5) Rooting culture:...

Embodiment 3

[0043] Embodiment 3 poplar explant detoxification method

[0044] (1) Obtaining explants: Select the robust stems of Populus sinensis 40 days after cutting as the source of explants, spray the surface of the explants with 75% ethanol, cut off the leaves and petioles, and keep 2 axillary buds.

[0045] (2) Soaking: After the explants are obtained, they are soaked in a solution containing 200 mg / L cephalosporin for 15 minutes.

[0046](3) Rinse: Rinse the soaked explants twice with a solution containing 200 mg / L cephalosporin, and dry the surface moisture with sterile filter paper or absorbent paper.

[0047] (4) Disinfection: Soak in 75% ethanol and sterile water containing 200 mg / L cephalosporin for 20 minutes in turn, blot the surface moisture with sterile filter paper or absorbent paper, and cut off the 1.5mm edge stem sections at both ends (cut off The rear stem section at both ends is 3cm) to complete the explant disinfection treatment.

[0048] (5) Rooting culture: the ...

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Abstract

The invention discloses a poplar explant detoxification reagent, a detoxification method and application, and belongs to the technical field of forest tree genetic breeding. The detoxification reagent comprises 75% ethanol and 100-200 mg / L cephalosporin solution, and the poplar explants are alternately treated by the two reagents. The invention further specifically discloses a specific method for detoxifying the poplar explants by using the detoxification reagent. According to the method, detoxification treatment can be completed only by using the ethanol with the concentration of 75% and cephalosporin, the method is simple and easy to implement, the problem that parasitic bacteria on the surfaces of poplar cutting seedlings are difficult to remove can be effectively solved, a large amount of poplar explant detoxification work can be completed more quickly, and time and manpower are saved; and in addition, the explants subjected to detoxification treatment through the method have high rooting performance, and a new working method and breeding material can be provided for vegetative propagation and tissue culture of poplar.

Description

technical field [0001] The invention relates to the technical field of tree genetics and breeding, and relates to a poplar explant detoxification agent, a detoxification method and an application thereof. Background technique [0002] The mountain new poplar variety is a polyploid poplar tree with stable offspring traits obtained after more than 20 years of hybridization with Populus aspen as the female parent and Populus Xinjiang as the male parent. The trunk is straight, the bark is smooth and light green, covered with white powder, and the infructescence falls off naturally without flying catkins. Compared with the control variety Xinjiang Poplar, this variety has stronger adaptability, better stress resistance and low temperature tolerance, and no freezing damage occurs when planted in most parts of the north. The rooting ability of Populus sinensis is poor, the survival rate of conventional cuttings is low, and various bacteria and fungi are parasitic on the surface an...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 陈肃于越刘智杰
Owner NORTHEAST FORESTRY UNIVERSITY
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