Cymbidium ssp. bud embryo composite microcrystalline capsule composition, preparation method thereof, and application of Cymbidium ssp. bud embryo composite microcrystalline capsule composition in promotion of cell detoxification, energy activation and aging resistance

A composition and germ technology, applied in energy activation and anti-aging, orchid germ compound microcrystalline capsule composition, the preparation method of the above composition and its use in the field of promoting cell detoxification can solve the problem of different extraction components and contents To avoid low boiling point and volatilization of effective substances, promote cell detoxification, and promote ATP generation

Pending Publication Date: 2021-12-10
OMGBIO INTL LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the parts of the orchids used in the above-mentioned cases are different. The first and second cases were carried out with the whole plant, while the third case only used the petals. Due to the different extraction methods, processes, equipment used, or environmental parameters, the obtained Extracted ingredients and content will be different

Method used

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  • Cymbidium ssp. bud embryo composite microcrystalline capsule composition, preparation method thereof, and application of Cymbidium ssp. bud embryo composite microcrystalline capsule composition in promotion of cell detoxification, energy activation and aging resistance
  • Cymbidium ssp. bud embryo composite microcrystalline capsule composition, preparation method thereof, and application of Cymbidium ssp. bud embryo composite microcrystalline capsule composition in promotion of cell detoxification, energy activation and aging resistance
  • Cymbidium ssp. bud embryo composite microcrystalline capsule composition, preparation method thereof, and application of Cymbidium ssp. bud embryo composite microcrystalline capsule composition in promotion of cell detoxification, energy activation and aging resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043]

[0044] From orchid terminal buds or side buds, extract growth point germ tissue. Secondly, adopt the "rapid liquid nitrogen freezing ultrasonic oscillation method", the specific operation is as follows: the germ tissue is rapidly frozen with liquid nitrogen to remove the wall membrane, and the tissue grinding bowl and tissue grinder are pre-set at -80°C to cool; Afterwards, use a knife to cut the tissue from which the wall membrane has been removed and put it into a grinding bowl, add a little liquid nitrogen to solidify the tissue block, and then grind it into powder; then, put the tissue powder into an ultrasonic vibration extraction device, and Cover and soak the tissue powder with pure water or ethanol; then, shake the mixture of solvent and powder at low temperature with a total energy of 300 to 600W for 60 minutes (3 minutes of shaking, rest for 2 minutes, until the shaking time reaches 60 minutes); After centrifugation at 15,000 rpm for 15 minutes at 4°C, the...

Embodiment 2

[0052]

[0053] d-alpha-tocopheryl polyethylene glycol 1000 succinate (d-alpha tocopheryl polyethylene glycol 1000 succinate, TPGS) is a vitamin E derivative, which is safe and has excellent oral biocompatibility with effective active ingredients. At present, TPGS is used in the drug delivery system of liposomes to increase the stability of liposomes. Previous studies used TPGS to form micelles as anticancer drug carriers. Previous studies have also added TPGS to the polymer carrier as a carrier for the controlled release of anticancer drugs (Cao & Feng, 2008). Therefore, TPGS is an excellent functional adjuvant for the microcrystalline capsule composition of this embodiment, and it also has anti-aging and anti-oxidation activities.

[0054] Specifically, this example uses hydrogenated soybean lecithin (hydrogenated soybean lecithin, HL) and d-α-tocopheryl polyethylene glycol 1000 succinate to prepare orchid germ composite microcrystalline capsule composition, the detailed ...

Embodiment 3

[0060]

[0061] Autophagy is to coat aging organelles or protein aggregates and send them to the lysosome for decomposition into small molecules. Aging occurs when cells are unable to resist the damage caused by oxidative stress.

[0062] Human skin keratinized cells HaCaT were used at a density of 1.5x10 5 The cells / mL were cultured in 96-well plates and grown for more than 24 hours at 37°C and 5% carbon dioxide incubator. Afterwards, the OG-L reaction was added, and after the reaction time, the supernatant was removed and washed with PBS. Then, after 20mJ / cm 2 After UVB irradiation, add fresh culture solution containing 0.1 mM hydrogen peroxide for 1 hour. Then, add autophagosome detection reagent working solution and place in the incubator for 15 minutes to 1 hour to react, then wash the cells 3 to 4 times with wash buffer. Finally, quantitative PBS was added, and the absorbance was measured with excitation light 360nm and emission light 520nm (BioTek, Synergy TM 2,...

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Abstract

The Cymbidium ssp. bud embryo composite microcrystalline capsule composition comprises: a carrier which is formed into a shell-like structure, and a Cymbidium ssp. bud embryo extract embedded in the shell-shaped structure or coated with the shell-shaped structure. The preparation method of the Cymbidium ssp. bud embryo extract comprises the following steps of: capturing growing point bud embryo tissues from terminal buds or lateral buds of Cymbidium ssp.; removing the wall membranes of the growing point bud embryo tissues; grinding the growing point bud embryo tissues into powder at low temperature; covering and soaking the powder with water or ethanol to obtain a mixed solution; oscillating the mixed solution at a low temperature by using ultrasonic oscillation extraction equipment at total energy of 300-600W; centrifuging the mixed solution to obtain supernate; and removing the solvent of the supernate. The composition provided by the invention can avoid deterioration of the Cymbidium ssp. bud embryo extract so as to provide a stable effect. In addition, the composition of the invention can promote cell detoxification, energy activation, glycation resistance and aging resistance.

Description

technical field [0001] The invention relates to a microcrystalline capsule composition, and in particular to a composite microcrystalline capsule composition of orchid germs. In addition, the present invention also relates to a preparation method of the above composition and its use for promoting cell detoxification, energy activation and anti-aging. Background technique [0002] Taiwan, China has mature orchid planting and tissue culture technology, which includes a wide variety of varieties, proficient tissue culture skills, virus detection capabilities, and engineering technology for mass production. Moreover, Taiwan, China currently has the advantages to promote the orchid industry, including unique natural geographical conditions, diversified varieties that dominate the world, a group of top-notch breeding experts, a super-level agricultural technology foundation, and agricultural experiment improvement and academic research all over the island. and other agricultural ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/9794A61K8/86A61K8/55A61K8/11A61Q19/00A61Q19/02A61Q19/08
CPCA61K8/9794A61K8/86A61K8/553A61K8/11A61Q19/00A61Q19/004A61Q19/02A61Q19/08A61K2800/412A61K2800/56
Inventor 陈丽玉
Owner OMGBIO INTL LTD
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