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Method for continuously preparing amino acid strain

An amino acid and bacterial species technology, applied in the biological field, can solve the problems of complex operation, affecting the results of amino acid fermentation, and easy to contaminate bacteria.

Inactive Publication Date: 2019-02-19
马彪
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, the inoculation method of amino acid fermentation generally adopts batch inoculation, that is, each batch uses a batch of seeds, and there are differences in seeds between different batches, which affects the results of amino acid fermentation
Most of the inoculation methods use the seed shaker bottle to pour into the seed tank under the protection of the flame, which has the problems of complicated operation and easy contamination

Method used

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Examples

Experimental program
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Effect test

Embodiment Construction

[0004] Embodiment: This embodiment is used to illustrate the method for continuous inoculation provided by the invention. The 20L stainless steel tank is used as a tank for cultivating seeds. The relevant supporting facilities are the same as the general stainless steel fermentation tank. The culture medium of this tank adopts the conventional medium with known amino acids, and the steam is automatically sterilized. The fed-batch medium is the same as the bottom material medium, and the pressure difference of the shake flask is used to inoculate; after inoculation, the culture is started under constant temperature, pH, and air volume conditions, and the culture volume is maintained at 10L. When the OD reaches 0.1 (10 times, 600nm ), start to feed the medium, and control the flow speed and discharge speed as consistent as possible, so that the OD is stable within 0.1-0.2 (10 times, 600nm), so as to ensure that the vitality of the bacteria does not decline. Embodiment 2: there a...

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Abstract

The invention discloses a method for continuously preparing an amino acid strain, and belongs to the technical field of biology. A mode of automatic and continuous inoculation is adopted, the inoculation efficiency is improved, the stability of the strain is maintained, the workload brought by preparation of the strain is reduced, and meanwhile complicated operation and the risk of bacterial contamination in existing inoculation modes are avoided.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for continuously preparing amino acid strains. Background technique [0002] At present, the inoculation method of amino acid fermentation generally adopts batch inoculation, that is, each batch uses a batch of seeds, and there are differences in seeds between different batches, which affects the results of amino acid fermentation. Most of the inoculation methods use seed shaking flasks to pour into seed tanks under the protection of flames, which has the problems of complicated operation and easy bacterial contamination. SUMMARY OF THE INVENTION Based on this, the inoculation method is improved, and the present invention provides a method for continuously preparing amino acid strains. Improve the inoculation efficiency, maintain the stability of the strain, reduce the workload caused by the preparation of the strain, and avoid the complicated operation and the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/04
CPCC12P13/04
Inventor 马彪
Owner 马彪
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