Manganese oxidizing composite microbial system and application thereof

A technology of complex bacteria and manganese oxidation, which is applied in the direction of bacteria, chemical instruments and methods, and the restoration of polluted soil, etc., to achieve the effect of broad application prospects

Active Publication Date: 2014-04-30
清华苏州环境创新研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The manganese oxidizing complex bacteria involved in the present invention have manganese oxidizing activity only when the two strains are cultured together, and have no manganese oxidizing activity when cultured alone. This special manganese oxidation phenomenon is discovered for the first time in the world

Method used

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  • Manganese oxidizing composite microbial system and application thereof
  • Manganese oxidizing composite microbial system and application thereof
  • Manganese oxidizing composite microbial system and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1. Isolation, purification and identification of Arthrobacter sp.QXT-31 and Sphingopyxis sp.QXT-31B

[0044] 1. Separation and purification of Arthrobacter sp.QXT-31 and Sphingopyxis sp.QXT-31B

[0045] Arthrobacter sp.QXT-31 and Sphingopyxis sp.QXT-31B were obtained from the manganese ore piled soil in Xiangtan, Hunan, after domestication, separation and purification. Among them, Arthrobacter sp.QXT-31 is a Gram-positive bacterium, and Sphingopyxis sp. .QXT-31B is Gram-negative bacteria. Specific steps are as follows:

[0046] Take the soil where manganese ore is piled up in Hunan Xiangtan Manganese Mine, and add 2g of the soil to a 100-fold diluted PYG (peptone-yeast extract-glucose) medium (each of peptone, glucose, and yeast extract) for high temperature sterilization (115°C, 25 minutes). 0.25g / L, CaCl 2 .2H 2 O content 0.01g / L, MgSO 4 .7H 2 O content 0.5g / L, MnCl 2 Content 100μM, deionized water 1L), the buffer in the medium is HEPES (N-2-Hydroxyethy...

Embodiment 2

[0062] Embodiment 2, the composite strain that comprises Arthrobacter sp.QXT-31 and Sphingopyxis sp.QXT-31B is to Mn 2+ Study on the biological oxidation characteristics of

[0063] 1. Composite strains including Arthrobacter sp.QXT-31 and Sphingopyxis sp.QXT-31B are effective against Mn 2+ biological oxidation

[0064] After adding Mn 2+ In the PYG medium of the present invention, investigate the compound strain of the present invention to Mn 2+ oxidation capacity. It is found that the compound bacterial strain of the present invention is effective against Mn 2+ It has good oxidation ability and can convert Mn 2+ All are oxidized to manganese oxides which are insoluble in water. Specific steps are as follows:

[0065] 1.1) get the bacterium liquid of 2mL composite bacterial system of the present invention, inoculate to 100mL does not contain Mn 2+ In the Erlenmeyer flask of the PYG medium, 30 ° C, 170 rpm shaking culture for 48 hours.

[0066] 1.2) Take 10 mL of the ...

Embodiment 3

[0085] Embodiment 3, the manganese oxidation effect of the complex bacterial system of the present invention in actual domestic wastewater

[0086] Add the composite bacterial system of the present invention to investigate Mn in actual domestic waste water 2+ Oxidation ability, it is found that the composite bacteria can adapt to the actual domestic wastewater, and can exert manganese oxidation activity in domestic wastewater. Specific steps are as follows:

[0087] The composite bacterial line prepared in step 1.1) in step 1 of Example 2 was centrifuged at 3000-4500rpm for 2 minutes to collect the thalline, and washed with PBS phosphate buffer (NaCl: 2.7mM; NaCl: 2.7mM; 2 HPO 4 : 10mM; KH 2 PO 4 : 2mM; pH 7.1) to wash the bacteria once, then centrifuge, add the bacteria to a 1000mL triangular flask, and then pour 600mL of actual domestic wastewater (randomly taken from the sewer of the living area of ​​the Eco-Environmental Research Center of the Chinese Academy of Scienc...

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Abstract

The invention discloses a composite microbial system capable of oxidizing Mn2+ and an application of the composite microbial system in a water body or a solid matrix. The composite microbial system can oxidize Mn2<+> in the water body or the solid matrix to generate manganese oxide insoluble in water. The Mn2<+> oxidizing composite microbial system comprises two bacterial strains belonging to different categories: Arthrobacter sp. QXT-31 and Sphingopyxis sp. QXT-31B, respectively. The preserve numbers are respectively CGMCC No.6631 and CMGCC No.6947. The Arthrobacter sp. QXT-31 and Sphingopyxis sp. QXT-31B provided by the invention are used to oxidize Mn2<+> in the natural water body, and the operating temperature (10-30 DEG C) is in a normal temperature range, the operating pH value (6.5-8.5) is in a neutral range, and high Mn2<+> oxidizing efficiency is obtained.

Description

technical field [0001] The invention relates to a manganese oxidizing compound bacterial strain and its application. Specifically, the composite strain includes Arthrobacter sp. QXT-31 and Sphingopyxis sp. QXT-31B, and their preservation numbers are: CGMCC No.6631 and CGMCC No.6947 . Background technique [0002] Divalent manganese ions (Mn 2+ ) is one of the trace elements necessary for the normal body, but long-term excessive intake can cause neurosis and autonomic dysfunction. The mining and consumption of manganese ore in my country maintain a high-speed growth trend, and a large amount of Mn is inevitably produced in this link. 2+ emissions and pollution, resulting in Mn in surface water 2+ pollution. In addition, due to geological structure and other reasons, Mn in groundwater in many places in our country 2+ Concentrations are often exceeded. while Mn 2+ It is stable under the conditions of natural temperature (less than 40°C) and pH value (less than 8), and o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/06C12R1/01C02F101/20
Inventor 曲久辉梁金松柏耀辉胡承志刘会娟刘锐平
Owner 清华苏州环境创新研究院
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