Lonicera japonica antibacterial agent for restraining micrococcus luteus

A technology of Micrococcus luteus and honeysuckle, which is applied in the directions of topical antibacterials, antibacterials, and resistance to vector-borne diseases, etc., can solve the problems of large dosage and affect the effect, and achieve the effect of reducing dosage and enhancing bacteriostatic performance.

Active Publication Date: 2014-11-19
安信生物科技(湖南)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Concentrated Chinese medicine preparations made into oral liquids or bacteriostatic agents, coating agents, etc. have a large one-time dosage, which affects the effect

Method used

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  • Lonicera japonica antibacterial agent for restraining micrococcus luteus
  • Lonicera japonica antibacterial agent for restraining micrococcus luteus
  • Lonicera japonica antibacterial agent for restraining micrococcus luteus

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Preparation process of honeysuckle extract: Weigh 50 g of dried honeysuckle, add 450 mL of water, extract at 95 °C for 4 h, and use a 0.22 μm filter to remove impurities and Bacteria. Finally, the content of chlorogenic acid in the extract was determined to be 23.62 mg / g by high performance liquid chromatography.

[0027] 2. Preparation of water-soluble silver nanoparticles:

[0028] Take 22.8 g of myristic acid and dissolve it in 140 mL of methanol and water mixed at a ratio of 2:5, add 4 g of sodium hydroxide to the solution to precipitate a precipitate, filter, add the precipitate to 100 mL of 10 mol / L water-soluble Silver myristate was obtained in a neutral silver nitrate solution; 6.7 g of silver myristate with a concentration of 20 mmoL / L was weighed in a 100 mL beaker, and 58 mL of triethylamine with a concentration of 40 mmoL / L was added to the beaker , under electromagnetic stirring at 80°C for 2 h, the white silver myristate powder gradually turned brown, ...

Embodiment 2

[0043] 1. The preparation process of honeysuckle extract:

[0044] With embodiment 1.

[0045] 2. Preparation of water-soluble silver nanoparticles

[0046] a. Take a certain amount of myristic acid and dissolve it in methanol and water mixed in a certain proportion, add a certain amount of sodium hydroxide to the solution to precipitate, filter, add the precipitate to the water-soluble silver nitrate solution to obtain tetradecane acid silver.

[0047] b. Weigh 6.7 g of 20 mmoL / L silver myristate in a 100 mL beaker, add 58 mL of triethylamine with a concentration of 40 mmoL / L to the beaker, stir for 2 h at 80 ° C, and the white tetradecanoate The silver alkanoate powder gradually turned brown, and the insoluble precursor disappeared, and 20 mL of acetone was added to precipitate and precipitate, filtered with suction, washed with acetone for several times, and dried in vacuum to obtain silver nanoparticle powder.

[0048] 3. Synergistic antibacterial effect of honeysuckle ...

Embodiment 3

[0061] 1. The preparation process of honeysuckle extract:

[0062] With embodiment 1.

[0063] 2. Preparation of water-soluble silver nanoparticles:

[0064] With embodiment 1.

[0065] 3. The synergistic antibacterial effect of honeysuckle extract and water-soluble nano-silver.

[0066] a. Culture of bacteria

[0067] Pick a single colony of Micrococcus luteus on the peptone agar medium of beef extract and put it in 5 mL of peptone liquid medium of beef extract, culture it in the medium at 30 ℃ for 24 h, and then inoculate it in 5 mL of beef at an inoculation amount of 1%. After culturing for 12 h at 30 °C in the peptone liquid medium. Take 1 mL of the above-mentioned bacterial solution and serially dilute three gradients in the mixture of PBS and liquid medium in a ratio of 4:1, and the final bacterial solution is about 10 6 ~10 7 CFU / mL.

[0068] b. The synergistic effect of water-soluble nano-silver and honeysuckle extract on antibacterial properties

[0069] Take...

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Abstract

The invention discloses a lonicera japonica antibacterial agent for restraining micrococcus luteus and belongs to the field of traditional Chinese medicine. The lonicera japonica antibacterial agent is characterized in that lonicera japonica extracting solution in the antimicrobial agent is mixed with water-soluble nanometer silver according to the ratio of 75:1 to 100:1, and through the combined use of the two drugs, a synergistic role in restraining the micrococcus luteus is played. Compared with the prior art, a lonicera japonica extract and a nanometer silver antibacterial agent in the lonicera japonica antibacterial agent solve the problem of higher dosage of the lonicera japonica antibacterial agent in a conventional antibacterial method; meanwhile, with the adoption of two low-dosage antibacterial substances, the occurrence probability of microorganism drug resistance is reduced, and the toxicity of the antibacterial substances to a human body is reduced, so that more safety is realized.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicines, in particular to a traditional Chinese medicine antibacterial combination. Background technique [0002] Honeysuckle (Lonicera japonica Thumb), also known as double flower, silver flower, and honeysuckle flower, is the flower bud of the perennial winding woody vine Lonicera japonica of Lonicera family. It is one of the most important members of Chinese herbal medicine resources and is distributed throughout the north and south of my country. Its physiological functions for the human body are mainly heat-clearing and detoxifying, evacuating wind-heat, cooling blood and stopping dysentery. Modern medical research shows that honeysuckle is rich in chlorogenic acid, isochlorogenic acid, flavonoids, honeysuckle, inositol and trace volatile oil, so it has positive effects on the human body. Honeysuckle can resist pathogenic microorganisms. Experiments have proved that honeysuckle is effect...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/355A61P31/04A61P31/02A61K33/38
CPCY02A50/30
Inventor 许恒毅魏华曲锋熊勇华赖卫华徐锋万翠香郭亮
Owner 安信生物科技(湖南)有限公司
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