Method for degrading oily sludge of well site by using microorganisms

Abstract

The invention relates to a method for degrading the oily sludge of a well site by using microorganisms. The method comprises the following steps of: obtaining four degrading bacteria namely pseudomonas aeruginosa, micrococcus luteus, bacillus subtilis and acinetobacter johnsonii from the oily sludge of the well site in the loess tableland region through culturing, separation, screening, domestication and mutation, and mixing the four degrading bacteria according to the weight ratio of 1:1:1:1 to obtain a microorganism flora for degrading the oily sludge, wherein the initial pH value equals to6, the weight ratio of carbon to nitrogen equals to 100:3, and the weight ratio of carbon to phosphorus equals to 100:0.6, the mass concentration of oils in a treatment system added with mixed bacteria is reduced from 17214 mg / kg to 1257 mg / kg within 72 h in a laboratory, and the degradation rate is 92.7%. After the oily sludge with 10.55% initial oil content is experimentally treated for 56 daysin the well site, the removal rate of oil matters can reach 89.1%, and the treatment effect is obvious.

Description

technical field [0001] The invention relates to a method for degrading the oily sludge at the well site by microorganisms obtained by culturing and screening the oily sludge at the well site of the loess tableland area. Background technique [0002] The composition of oily sludge is complex and difficult to deal with. At present, the oily sludge treatment technologies adopted at home and abroad can be roughly divided into three categories: physical and chemical treatment, biological treatment and comprehensive utilization technology. Among them, physical and chemical treatment and comprehensive utilization technologies are difficult to be put into large-scale application because of the limitations of secondary pollution, complex process, high treatment cost, and difficult equipment maintenance. [0003] There are two types of biological treatment methods. One is to add nutrients to oily sludge and aerate to promote the growth and proliferation of sludge indigenous microorga...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a group of bacterial strains with high-efficiency degradation ability to different component compounds in crude oil, and to construct a microbial flora with high-efficiency repairing effect on oily sludge in loess tableland oil field, which is used to solve the problem of loess plateau area. The problem of low-concentration oily sludge pollution in well sites in the natural environment of scattered oilfield well sites and inconvenient transportation