Method for degrading oily sludge of well site by using microorganisms

A technology for microbial degradation and oil sludge, applied in the directions of microorganism-based methods, biological sludge treatment, biochemical equipment and methods, etc., can solve the problems of inconvenient transportation, low-concentration oily sludge pollution at well sites, and dispersion of oilfield well sites. , to achieve the effect of not causing secondary pollution, saving transportation and reducing content

Active Publication Date: 2012-05-23
PETROCHINA CO LTD
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a group of bacterial strains with high-efficiency degradation ability to different component compounds in crude oil, and to construct a microbial flora with high-efficiency repairing effect on oily

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The synergistic effect of microorganisms was studied, and the oily sludge degradation ability was analyzed and studied according to the synergistic effect of different bacterial species. The ratio of 1:1:1:1 was mixed. In the laboratory, within 72 hours, the mass concentration of petroleum in the treatment system added with mixed bacteria decreased from 17214mg / kg to 1257mg / kg, and the degradation rate was 92.7%, which was higher than that of the control group. The oil degradation rate increased by 57.3%.

Embodiment 2

[0018] A 56-day treatment test was carried out on sludge with an oil content of 10.55% on site

[0019] 1. Composition of efficient degrading bacteria community

[0020] Four kinds of degrading bacteria, Pseudomonas aeruginosa, Micrococcus luteus, Bacillus subtilis and Acinetobacter jonesi, were obtained from the oily sludge of the oil field in the loess plain area through cultivation, separation, screening, domestication and mutagenesis. 1:1:1:1 mixed composition of additives;

[0021] 2. Suitable degradation conditions

[0022] The suitable degradation conditions for the mixed microbial flora are initial pH value = 6, the weight ratio of carbon to nitrogen is equal to 100:3, the weight ratio of carbon to phosphorus is equal to 100:0.6, and the temperature is 30°C. Other conditions are not strictly limited, provided by the natural conditions of the loess plateau .

[0023] 3. Implementation steps

[0024] (1) Move the oil sludge contaminated by crude oil on the well site ...

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Abstract

The invention relates to a method for degrading the oily sludge of a well site by using microorganisms. The method comprises the following steps of: obtaining four degrading bacteria namely pseudomonas aeruginosa, micrococcus luteus, bacillus subtilis and acinetobacter johnsonii from the oily sludge of the well site in the loess tableland region through culturing, separation, screening, domestication and mutation, and mixing the four degrading bacteria according to the weight ratio of 1:1:1:1 to obtain a microorganism flora for degrading the oily sludge, wherein the initial pH value equals to6, the weight ratio of carbon to nitrogen equals to 100:3, and the weight ratio of carbon to phosphorus equals to 100:0.6, the mass concentration of oils in a treatment system added with mixed bacteria is reduced from 17214 mg/kg to 1257 mg/kg within 72 h in a laboratory, and the degradation rate is 92.7%. After the oily sludge with 10.55% initial oil content is experimentally treated for 56 daysin the well site, the removal rate of oil matters can reach 89.1%, and the treatment effect is obvious.

Description

technical field [0001] The invention relates to a method for degrading the oily sludge at the well site by microorganisms obtained by culturing and screening the oily sludge at the well site of the loess tableland area. Background technique [0002] The composition of oily sludge is complex and difficult to deal with. At present, the oily sludge treatment technologies adopted at home and abroad can be roughly divided into three categories: physical and chemical treatment, biological treatment and comprehensive utilization technology. Among them, physical and chemical treatment and comprehensive utilization technologies are difficult to be put into large-scale application because of the limitations of secondary pollution, complex process, high treatment cost, and difficult equipment maintenance. [0003] There are two types of biological treatment methods. One is to add nutrients to oily sludge and aerate to promote the growth and proliferation of sludge indigenous microorga...

Claims

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Application Information

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IPC IPC(8): C02F11/02C12N1/20C12R1/125
Inventor 周立辉曾亚勤任建科张海玲杨琴冀忠伦李岩张璇赵敏
Owner PETROCHINA CO LTD
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