A 1-deoxynojirimycin preparing method suitable for industrial production comprises the following steps: (1)
raw material pre-treatment: crushing the dry
mulberry leaf raw material; (2) enzymelysis: adding water into
powder, raising the temperature, adding a combined
enzyme for enzymelysis, and filtering; (3) continuous combined extraction: performing continuous combined extraction on filter residues by using 3-4 extracting devices, mixing extracting solutions, and filtering; (4)
alcohol precipitation and
centrifugation: performing pressure-reduction concentration to 1 / 3-1 / 2 of the original volume, cooling, adjusting pH, adding an
alcohol solution, stirring, performing still standing, and centrifuging by using a tube
centrifuge; (5) membrane
filtration: at 25-35 bar pressure at
room temperature, ultrafiltering by using an organic
ultrafiltration membrane, and then at 0.5-1.5 MPa pressure at
room temperature, nano-filtering by using a
nanofiltration membrane; (6) concentrating; (7)
drying. By using the 1-deoxynojirimycin preparing method, the loss of a
solvent can be reduced to a large extent, the
energy consumption can be reduced and the product yield can be increased; therefore, the 1-deoxynojirimycin preparing method is suitable for industrial production.