30 results about "Acinetobacter johnsonii" patented technology

The invention relates to a method for degrading the oily sludge of a well site by using microorganisms. The method comprises the following steps of: obtaining four degrading bacteria namely pseudomonas aeruginosa, micrococcus luteus, bacillus subtilis and acinetobacter johnsonii from the oily sludge of the well site in the loess tableland region through culturing, separation, screening, domestication and mutation, and mixing the four degrading bacteria according to the weight ratio of 1:1:1:1 to obtain a microorganism flora for degrading the oily sludge, wherein the initial pH value equals to6, the weight ratio of carbon to nitrogen equals to 100:3, and the weight ratio of carbon to phosphorus equals to 100:0.6, the mass concentration of oils in a treatment system added with mixed bacteria is reduced from 17214 mg/kg to 1257 mg/kg within 72 h in a laboratory, and the degradation rate is 92.7%. After the oily sludge with 10.55% initial oil content is experimentally treated for 56 daysin the well site, the removal rate of oil matters can reach 89.1%, and the treatment effect is obvious.

The invention discloses an acinetobacter johnsonii strain capable of carrying out biological denitrification under a low temperature and an application thereof. The acinetobacter johnsonii strain HA7 provided by the invention was collected in the China General Microbiological Culture Collection Center (CGMCC) on September 15, 2010, with the collection number of CGMCC No.4168. At the temperature of 10-20 DEG C, the strain is capable of carrying out biological denitrification as long as the strain is put into the nitrogenous wastewater with dissolved oxygen of 2-6mg/L. The strain has the following beneficial effects: the strain has strong tolerance to low temperature, can grow well under a low temperature and simultaneously has the capabilities of heterotrophic nitrification and aerobic denitrification; and the strain can carry out nitrification and denitrification synchronously under low temperature and aerobic conditions to efficiently and thoroughly remove the total nitrogen in the sewage, thus effectively solving the great difficulty in carrying out biological denitrification under a low temperature and having a good application prospect.

The invention belongs to the field of environmental microorganisms and in particular relates to separation and culture of an Acinetobacter johnsonii AJ-3 strain and application thereof in phosphorus removal in sewage and biological phosphorus removal in urban sewage treatment plants. The Acinetobacter johnsonii AJ-3 strain has a collection number of CCTCCM2014023, the collection place is China Center for Type Culture Collection, and the collection date is January, 16th 2014. The strain is an Acinetobacter johnsonii AJ-3 strain, is rapid in growth, high in adaptive capacity to environment and low in dissolved oxygen demands and can rapidly grow in a phosphorus-containing synthetic sewage liquid culture medium, the phosphorus is removed, the phosphorus removal efficiency is high and stable and reaches 55-60.1%, and secondary pollution is avoided. Therefore, the AJ-3 strain can be applied to phosphorus-containing sewage treatment, is an important microbial resource in a water organism phosphorus removal process and has wide application prospect.

The invention relates to acinetobacter johnsonii LP28 and a method for preparing low-temperature alkali lipase by using the acinetobacter johnsonii. The strain is preserved in CGMCC, the preservation number is CGMCC 3069, and the homology of a 16S rDNA sequence of the strain and DQ257426 is as high as 100 percent. The method for preparing the low-temperature alkali lipase comprises the following steps: (1) screening bacterial strains; (2) seed cultivation; (3) fermentation and cultivation; and (4) separation and purification. The acinetobacter johnsonii LP28 is from the soil polluted by oil in a saline and alkaline land of Tianjin Bohai Bay, the enzyme production is stable, the raw material source is wide, and the production cost is low; and the obtained low-temperature alkali lipase has good stability for additive components of a washing agent such as surfactant, oxidant, protease and the like, and can be used as enzyme added into the washing agent to be applied in the industry of the washing agent.

The invention provides a composition or composite bacterium agent for treating waste well drilling mud. The invention provides a composition, a composite bacterium agent and application thereof. The composition or the composite bacterium agent comprises bacillus subtilis, pseudomonas stutzeri and acinetobacter johnsonii and is used for treating the waste well drilling mud.

The present invention relates to the technical field of the preparation of microecological preparations, particularly to a microecological preparation and a preparation method thereof, wherein the raw materials comprise Bacillus subtilis, Acinetobacter johnsonii, lactobacilli and yeast. The preparation method comprises: respectively activating Bacillus subtilis, Acinetobacter johnsonii, lactobacilli and yeast in freezing storage tubes in slopes, and purifying to various slope strains so as to be spare; preparing corresponding raw bacterial liquids from various slope strains; inoculating various raw bacterial liquids onto a sterilized fermentation culture medium I according to a fixed bacterial ratio, and culturing to obtain the primary seed of a composite microecological preparation; inoculating the primary seed onto a sterilized fermentation culture medium II, and culturing to obtain the secondary seed of the composite microecological preparation; and inoculating the secondary seed onto a sterilized fermentation culture medium III, and culturing to obtain the composite microecological preparation finished product. According to the present invention, the composite microecological preparation can effectively reduce nitrogen and phosphorus in the sewage, and can reduce the eutrophication degree of the sewage.

The invention provides a composite microbial agent capable of reducing a COD value of heavy-polluted wastewater. The composite microbial agent is characterized by comprising the following ingredients in parts by weight: 1-8.5 parts of Pseudomonas putida, 2-8 parts of Rhodococcus globerulus, 2-5 parts of Rhodospirillum rubrum, 5-10 parts of Clostridium beijerinckii, 1-5 parts of flocculating candida, 2-5 parts of Acinetobacter johnsonii, 5-12 parts of Acetobacter pasteurianus, 13-34.5 parts of hybrid bacillus and 22.5-48 parts of hybrid Lactobacilli. According to the composite microbial agent provided by the invention, the degradation efficacy and impact resistance to a degraded substrate are strengthened by using synergism of a variety of strain ingredient populations; and a degradation method is high in pertinency, high in response speed and simple in operation.

The invention relates to the technical field of livestock breeding and particularly relates to a wild pepper meal pig feed and preparation method thereof. According to the preparation method, the wildpepper meal pig feed is prepared from the main raw material of wild pepper meal and the auxiliaries of resin enzyme, laccase, calendula, grape seeds, borage, willow bark, evening primrose, thyme andbirch leaves in a specific proportion, the wild pepper meal is fermented by virtue of candida mycoderma bacteria, torulopsis utilis, bacillus subtilis and Acinetobacter johnsonii, so that the amountsof organic matters, alkali-hydrolyzable nitrogen, rapid available phosphorus and available potassium in the wild pepper meal are increased, a small amount of toxic substances in the wild pepper meal are removed, the pungent flavor of the wild pepper meal is eliminated, a small amount of residual wax can hardly influence a digestive system, the effects of the raw materials are adequately exerted, finally, the meat quality of live pigs is improved, and the intramscular fat content and fatty acid content are increased; and the wild pepper meal pig feed is mainly prepared from the wild pepper meal, has good palatability and safety and is balanced in nutrition.

The invention provides a bacterial strain reproduction method for accelerating restoration of eutrophic water. Acinetobacter, nitrogen and phosphorus removal bacteria, achromobacter xylosoxidans, saccharomyces cerevisiae strains, acinetobacter johnson and pseudomonas can exert different functions in the water and achieve the synergistic effect and mutual promotion, total nitrogen and total phosphorus in the water are effectively removed, the eutrophic water is purified, and growth of pathogenic bacteria can also be inhibited; a culture medium can activate the acinetobacter, the nitrogen and phosphorus removal bacteria, the achromobacter, the xylosoxidans, the saccharomyces cerevisiae strains, the acinetobacter johnson and the pseudomonas in the dormant state and promote reproduction; an enzyme is beneficial for the reproduction of the acinetobacter, the nitrogen and phosphorus removal bacteria, the achromobacter, the xylosoxidans, the saccharomyces cerevisiae strains, the acinetobacterjohnson and the pseudomonas, and prolongs the proliferative period of the acinetobacter, the nitrogen and phosphorus removal bacteria, the achromobacter, the xylosoxidans, the saccharomyces cerevisiae strains, the acinetobacter johnson and the pseudomonas, and the speed of purifying the eutrophic water is increased. According to the method, the proliferative period of the bacterial strains for eliminating eutrophication can be prolonged, and the reproduction is accelerated, which is beneficial for increasing the speed of restoration for the eutrophic water.

The invention discloses a microbial preparation for low-temperature soil repair. The compound microbial preparation comprises a cold-resistant pseudogymnoascus roseus HD1031 bactericide, an aerobic denitrifying cold-resistant acinetobacter johnsonii DBP-3 bactericide and immobilized oxidordeuctase, wherein the immobilized oxidordeuctase consists of immobilized horseradish peroxidase and immobilized soil polyphenol oxidase. The invention further discloses a preparation method and application of the microbial preparation. The microbial preparation for low-temperature soil repair has a removal rate of 86% or more of a pentazolol pesticide and an organophosphorus pesticide and a removal rate of 85% or more of ammonia nitrogen, which are much higher than removal rates in the prior art and a comparison example of the present invention. Thus, the microbial preparation for low-temperature soil repair can effectively repair polluted soil. The preparation method is simple and is suitable for large-scale production. Benefits are brought for remote low-temperature regions. The problem of environmental pollution that urgently needs to be solved is solved.

The invention belongs to the field of construction materials, and particularly relates to a method for preparing a plate from construction waste. The method comprises the steps: the construction wasteis crushed and then subjected to high temperature treatment, then wetted through water, and treated through a microbial agent prepared from thiobacillus ferrooxidans, ferrooxidans sulfobacillus, lactobacillus, acinetobacter johnsonii, pseudomonas putida and cupriavidus metallidurans; liquid is removed, ingredients such as wood fiber pulp, calcium hydroxide, calcium silicate, peach gum, cement andwater are added into a solid product, and then a high temperature reaction is conducted; and finally the mixture is cut into the suitable size, high-pressure and high-temperature steam-curing is conducted, and finally the fiber plate which is low in impurity content, less in heavy metal and organic impurity residue, good in property such as strength, toughness, fire resistance and permeability resistance, and more durable is obtained.

The invention provides a preparation method of a composite microbial preparation for degrading high-oil-content kitchen waste. The composite microbial preparation comprises acinetobacter johnsonii, candida utilis, phanerochaete chrysosporium, bacillus subtilis and geobacillus stearothermophilus. The method comprises the following steps of S1, inoculating strains subjected to activation culture to a fermentation culture medium according to the mass percent of 5%, and performing fermentation culture, and S2, mixing the thalli cultured in the step S1 to prepare liquid mixed bacteria, mixing the liquid mixed bacteria with a solid adsorption carrier according to a mass ratio of 1: (1.5-2), and carrying out carrier adsorption and drying to obtain a composite microbial preparation. The liquid mixed bacteria comprise 30 to 40 percent of acinetobacter johnsonii, 10 to 20 percent of candida utilis, 10 to 15 percent of phanerochaete chrysosporium, 10 to 20 percent of bacillus subtilis and 10 to 20 percent of geobacillus stearothermophilus. Macromolecular compounds such as animal fat, vegetable fat and cellulose which are not easy to decompose in the kitchen waste can be efficiently and rapidly decomposed, and the degradation rate of protein and starch is maintained.

The invention discloses a degrading strain for bifenthrin insecticides and application thereof. The present invention relates to a method for producing a bacterial strain of Acinetobacter johnsonii, which is a strain F2 of Acinetobacter johnsonii. The strain can effectively degrade bifenthrin and other bifenthrin insecticides in a short time, has a remarkable biodegradation effect, can be used for repairing water bodies, soil and other natural environments polluted by the bifenthrin and other bifenthrin insecticides, can be directly applied to reduce the residual quantity of the bifenthrin insecticides in the water bodies and the soil to 85.84%, the problems of excessive residues of bifenthrin insecticides and environmental pollution in agricultural production are solved, and the method has a wide application prospect.

The invention aims to provide a method for processing emulsified oil in soybean oil waste water, and belongs to the technical field of microbial technology and environmental engineering. According to the method provided by the invention, acinetobacter calcoaceticus is used for performing emulsion-breaking treatment on the emulsified oil in the soybean oil waste water; then acinetobacter johnsonii is used for producing a microbial flocculant for performing flocculation treatment on the emulsion-broken emulsified oil to remove the emulsified oil in the soybean oil waste water. The method comprises the following steps: (1) preparing a bacterial suspension of acinetobacter calcoaceticus; (2) preparing the microbial flocculant with acinetobacter johnsonii; (3) performing emulsion-breaking treatment on the emulsified oil in the soybean oil waste water; (4) processing the emulsion-broken emulsified oil. The method provided by the invention has a high emulsified oil emulsion-breaking rate kept at 95.39%-96.70%; the microbial flocculant produced by the acinetobacter johnsonii is used for flocculation removal of the emulsion-broken emulsified oil, so as to achieve a higher removal rate of the emulsified oil in the soybean oil waste water, kept at 92.01%-94.45%, and also achieve a higher removal rate of COD in the soybean oil waste water, kept at 47.32%-50.88%.

The invention relates to the technical field of microorganisms, and particularly discloses a microbial agent suitable for corn planting, and application thereof. The microbial agent disclosed by the invention comprises at least one of the following strains: acinetobacter johnsonii, acinetobacter lwoffii, brevundimonas natans, burkholderia cepacia, microbacterium paraoxidans, pseudomonas aeruginosa, pseudomonas oryzae, pseudomonas ferrawiae, pseudomonas nitroreducens, pseudomonas formosana, rhodococcus ruber. serratia marcescens and neosphingosine resinophila. The microbial agent provided by the invention can promote absorption of beneficial mineral elements by corn, improves the problem of enrichment of heavy metals in soil by corn in an existing planting system, and reduces the heavy metal content of corn; moreover, compared with a conventional corn planting method, a planting method in the invention has the advantages that the corn germination speed and germination rate can be better increased, and the development condition of the root system of the corn in the seedling stage and the leaf surface development condition of the corn in the seedling stage can be improved.

The invention discloses a specific primer and a method for detecting Acinetobacter johnsonii and application, wherein the specific primer is as shown in SEQ ID NO. 1 to 2; the method includes the steps of extracting genomic DNA of a sample to be detected, using the primer for PCR amplification, performing gel electrophoresis detection, and photographing under a gel imaging system for detection, finding that a 365 bp DNA-specific band exists, which can determine that the sample contains the Acinetobacter johnsonii. The detection method of the invention has the advantages of short time, high specificity and high sensitivity in detecting the Acinetobacter johnsonii. A traditional identification method that is cumbersome in operation, long time-consuming, low in accuracy and low in detection rate is not used.

The present invention discloses a novel endophytic fungi, Ovatospora brasiliensis MTCC 25236 for the bioconversion of curcuminoids to Calebin-A and a method for its isolation from the rhizomes of Curcuma sp. The invention also discloses a method for the bioconversion of curcuminoids to Calebin-A using an endophytic fungi Ovatospora brasiliensis MTCC 25236 and bacterial species, Acinetobacter johnsonii and Pseudomonas putida.

The invention discloses an Acinetobacter johnsonii EAb-1 and an application thereof. The Acinetobacter johnsonii EAb-1 is collected in the China General Microbiological Culture Collection Center on May 5, 2017, and the collection number is CGMCC No. 14101. The Acinetobacter johnsonii EAb-1 is applied to heterorhabditis megidis prevention, has excellent killing activity, is applied to promotion of Ageratina adenophora growth and can effectively promote growth of crofton weeds.

The invention provides a method for improving the breeding speed of water body purifying bacterial colonies. Acinetobacter johnsonii, a saccharomyces cerevisiae strain, raoultella, pseudomonas mendocina and actinomycetes can remove COD, total phosphorus, total nitrogen, heavy metals and other pollutants in a water body, so that the water body purifying effect is improved, wherein an activation culture medium can be used for activating the acinetobacter johnsonii, the saccharomyces cerevisiae strain, the raoultella, the pseudomonas mendocina and the actinomycetes in a dormant state and promoting propagation; a propagation nutrient solution is beneficial to the acinetobacter johnsonii, the saccharomyces cerevisiae strain, the raoultella, the pseudomonas mendocina and the actinomycetes, prolongs the proliferative stage and improves the water body purifying speed. The method can promote breeding of the water body purifying bacterial colonies, the proliferative stage of the bacterial colonies can be prolonged, and purification of the water body can be accelerated.