30 results about "Acinetobacter johnsonii" patented technology

The invention discloses an acinetobacter johnsonii strain capable of carrying out biological denitrification under a low temperature and an application thereof. The acinetobacter johnsonii strain HA7 provided by the invention was collected in the China General Microbiological Culture Collection Center (CGMCC) on September 15, 2010, with the collection number of CGMCC No.4168. At the temperature of 10-20 DEG C, the strain is capable of carrying out biological denitrification as long as the strain is put into the nitrogenous wastewater with dissolved oxygen of 2-6mg / L. The strain has the following beneficial effects: the strain has strong tolerance to low temperature, can grow well under a low temperature and simultaneously has the capabilities of heterotrophic nitrification and aerobic denitrification; and the strain can carry out nitrification and denitrification synchronously under low temperature and aerobic conditions to efficiently and thoroughly remove the total nitrogen in the sewage, thus effectively solving the great difficulty in carrying out biological denitrification under a low temperature and having a good application prospect.

The invention provides a method for removing mechanical impurities in the dirty oil with microorganism, solving the problem of undesirable effect for removing the mechanical impurities in the dirty oil in the existing method for purifying the dirty oil. The method comprises the steps of: (1) inoculating acinetobacter johnsonii into a liquid LB culture medium to carry out accumulation culture, and controlling the cell concentration of culture solution OD=1.1-1.2; and (2) mixing the dirty oil with water according a certain volume ratio, heating up oil water mixture at 37 DEG C and keeping temperature, adding the culture solution obtained by the step (1) into the oil water mixture according to the inoculating quantity, the volume of which is 2-10% of that of the oil water mixture, simultaneously aerating, heating up at 60 DEG C to 90 DEG C after aerating and keeping temperature for 6-8 hours, and stopping heating up. The method can effectively reduce the content of the mechanical impurities in the dirty oil, thereby preventing the mechanical impurities from forming into deposition in a pipe line or equipment and being capable of guaranteeing the running stability of a joint station.

The invention relates to a method for degrading the oily sludge of a well site by using microorganisms. The method comprises the following steps of: obtaining four degrading bacteria namely pseudomonas aeruginosa, micrococcus luteus, bacillus subtilis and acinetobacter johnsonii from the oily sludge of the well site in the loess tableland region through culturing, separation, screening, domestication and mutation, and mixing the four degrading bacteria according to the weight ratio of 1:1:1:1 to obtain a microorganism flora for degrading the oily sludge, wherein the initial pH value equals to6, the weight ratio of carbon to nitrogen equals to 100:3, and the weight ratio of carbon to phosphorus equals to 100:0.6, the mass concentration of oils in a treatment system added with mixed bacteria is reduced from 17214 mg / kg to 1257 mg / kg within 72 h in a laboratory, and the degradation rate is 92.7%. After the oily sludge with 10.55% initial oil content is experimentally treated for 56 daysin the well site, the removal rate of oil matters can reach 89.1%, and the treatment effect is obvious.

The invention provides a composition or composite bacterium agent for treating waste well drilling mud. The invention provides a composition, a composite bacterium agent and application thereof. The composition or the composite bacterium agent comprises bacillus subtilis, pseudomonas stutzeri and acinetobacter johnsonii and is used for treating the waste well drilling mud.

The invention discloses a fully inactivated vaccine for preventing and curing giant spiny frog canker. A preparation method of the fully inactivated vaccine comprises the following steps: (1) taking acinetobacter junii, acinetobacter lwoffii and acinetobacter johnsonii as pathogenic bacteria, and treating each pathogenic bacterium in such a way that a formaldehyde solution is added into a pathogenic bacterium suspension with the OD600 value of 0.2 to prepare a vaccine with the formaldehyde concentration of 0.2% and the vaccine is inactivated in an incubator at 37 DEG C for 96 h, thus obtaining the formaldehyde inactivated vaccines of the three pathogenic bacteria respectively; (2) washing and then centrifuging the formaldehyde inactivated vaccines of the three pathogenic bacteria so as to obtain the treated inactivated vaccines of the three pathogenic bacteria respectively; and (3) mixing the treated inactivated vaccines of the three pathogenic bacteria at the volume ratio of 1:1:1, and then concentrating the mixture to 1 / 5 of the original total volume to obtain the fully inactivated vaccine for preventing and curing giant spiny frog canker. The fully inactivated vaccine provided by the invention can be used for preventing and curing giant spiny frog canker and has high popularization and application values.

The invention relates to acinetobacter johnsonii LP28 and a method for preparing low-temperature alkali lipase by using the acinetobacter johnsonii. The strain is preserved in CGMCC, the preservation number is CGMCC 3069, and the homology of a 16S rDNA sequence of the strain and DQ257426 is as high as 100 percent. The method for preparing the low-temperature alkali lipase comprises the following steps: (1) screening bacterial strains; (2) seed cultivation; (3) fermentation and cultivation; and (4) separation and purification. The acinetobacter johnsonii LP28 is from the soil polluted by oil in a saline and alkaline land of Tianjin Bohai Bay, the enzyme production is stable, the raw material source is wide, and the production cost is low; and the obtained low-temperature alkali lipase has good stability for additive components of a washing agent such as surfactant, oxidant, protease and the like, and can be used as enzyme added into the washing agent to be applied in the industry of the washing agent.