Acinetobacter johnsonii LP28 and method for preparing low-temperature alkali lipase by using acinetobacter johnsonii

A technology of Acinetobacter johnsonii and lipase, applied in the biological field

Inactive Publication Date: 2012-07-04
TIANJIN UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0008]At present, the research work on low-temperature alkaline lipase at home and abroad has just started. The low-temperature lipase studied is mainly produced by cold-tolerant microorganisms, and there are few strains available for research , its performance and suitability for industrialized production methods have not yet had a sound technical system

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  • Acinetobacter johnsonii LP28 and method for preparing low-temperature alkali lipase by using acinetobacter johnsonii
  • Acinetobacter johnsonii LP28 and method for preparing low-temperature alkali lipase by using acinetobacter johnsonii
  • Acinetobacter johnsonii LP28 and method for preparing low-temperature alkali lipase by using acinetobacter johnsonii

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Embodiment Construction

[0034] Below in conjunction with the examples, the present invention is further described, the following examples are illustrative, not limiting, and the protection scope of the present invention cannot be limited by the following examples.

[0035] 1. Isolation of Acinetobacter johnsonii LP28 strain

[0036] The present invention obtains a batch of lipase-producing microbial strains by using soybean oil as a carbon source, cultivating, separating and screening microbial strains on oil-contaminated soil samples in the saline-alkali land of Bohai Bay, and isolates a low-temperature Alkaline lipase-producing strain, coded as LP28, has excellent characteristics of producing low-temperature alkaline lipase. The 16S rDNA sequence of LP28 was compared with the homologous sequence in Genebank, and the homology between the 16S rDNA sequence of LP28 and DQ257426 was as high as 100%, so it was identified as Acinetobacter johnsonii. The strain has been preserved in the General Microorga...

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Abstract

The invention relates to acinetobacter johnsonii LP28 and a method for preparing low-temperature alkali lipase by using the acinetobacter johnsonii. The strain is preserved in CGMCC, the preservation number is CGMCC 3069, and the homology of a 16S rDNA sequence of the strain and DQ257426 is as high as 100 percent. The method for preparing the low-temperature alkali lipase comprises the following steps: (1) screening bacterial strains; (2) seed cultivation; (3) fermentation and cultivation; and (4) separation and purification. The acinetobacter johnsonii LP28 is from the soil polluted by oil in a saline and alkaline land of Tianjin Bohai Bay, the enzyme production is stable, the raw material source is wide, and the production cost is low; and the obtained low-temperature alkali lipase has good stability for additive components of a washing agent such as surfactant, oxidant, protease and the like, and can be used as enzyme added into the washing agent to be applied in the industry of the washing agent.

Description

Technical field [0001] The present invention is a method of biotechnology, especially a method of LP28 of Jacobacterius, and the use of this bacteria to prepare low temperature alkalinic lipase. Background technique [0002] Lipase (Lipase) is called a hydrolytic enzymes of glycerin, which is mainly used for hydrolysis and synthesis of triglyceride formed by the insoluble long -chain fatty acids above glycerin and 12 carbon atoms.It can only be hydrolyzed at the water-water interface of the heterogeneous or insoluble system.According to the principles of naming the International Biochemical Federation, the lipase number is EC 3.1.1.3, the system name is trimatolcelcetyl hydrolytic enzyme, and the habit of the name is lipase. [0003] Lipase hydrolysis has the advantages of efficient, mild response conditions, and non -toxic.It does not require coenzyme to catalyze the triglyceride hydrolace hydrolysis of glycerin and long-chain fatty acids in the water interface. The products of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N9/20C12R1/01
Inventor 戚薇王海宽邵静马会景魏玉洁
Owner TIANJIN UNIV OF SCI & TECH
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