Method for purifying dirty oil with microorganism

A technology for mechanical impurities and microorganisms, applied in biological water/sewage treatment, sustainable biological treatment, chemical instruments and methods, etc., can solve problems such as unsatisfactory removal of mechanical impurities, and achieve stable, guaranteed operation, and prevention of siltation. Effect

Active Publication Date: 2011-06-08
DAQING OILFIELD CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problem of unsatisfactory removal effect of mechanical impurities in dirty oil in existing dirty oil purification treatment methods, and to provide a method for using microorganisms to remove mechanical impurities in dirty oil

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0007] Embodiment one: the steps of the method for utilizing microorganisms to remove mechanical impurities in dirty oil of the present embodiment are as follows: (1) pick Acinetobacter johnsonii (Acinetobacter johnsonii) on the slant medium with an inoculation needle (bacteria are purchased from the Chinese Academy of Sciences Institute of Microbiology) was inoculated into liquid LB medium, and the medium was placed in a shaker at 37°C and 200r / min for 12 hours to obtain the primary culture solution; use a pipette to pipette the primary culture medium with a 2% inoculum Put the secondary culture medium in a shaker at 37°C and 200r / min for secondary culture, and control the cell concentration OD of the secondary culture medium. 600 =1.1~1.2; (two) take a sample to detect the content of mechanical impurities in the dirty oil, then mix the dirty oil with water at a volume ratio of 0.1:1, then heat the oil-water mixture to 37°C and keep the temperature constant, and the The secon...

specific Embodiment approach 2

[0009] Specific embodiment two: the steps of the method for utilizing microorganisms to remove mechanical impurities in dirty oil of the present embodiment are as follows: (1) pick the Acinetobacter johnsonii on the slant medium with an inoculation needle and inoculate it into the liquid LB medium, Put the culture medium in a shaker at 37°C and 200r / min for 12 hours to obtain the primary culture solution; pipette the primary culture solution with a 2% inoculation amount into a new liquid LB medium for secondary culture , place the secondary culture medium in a shaker at 37°C and 200r / min for shaking culture and control the cell concentration OD of the secondary culture medium 600 =1.1~1.2; (2) take a sample to detect the content of mechanical impurities in the waste oil, mix the waste oil with water at a volume ratio of 1:1, then heat the oil-water mixture to 37°C and keep the temperature constant, and obtain in step (1) The secondary culture solution is added in the oil-water...

specific Embodiment approach 3

[0011] Specific embodiment three: the steps of the method for utilizing microorganisms to remove mechanical impurities in dirty oil of the present embodiment are as follows: (1) pick the Acinetobacter johnsonii on the slant medium with an inoculation needle and inoculate it into the liquid LB medium, and Put the culture medium in a shaker at 37°C and 200r / min for 12 hours to obtain the primary culture solution; pipette the primary culture solution with a 2% inoculation amount into a new liquid LB medium for secondary culture , place the secondary culture medium in a shaker at 37°C and 200r / min for shaking culture and control the cell concentration OD of the secondary culture medium 600 =1.1~1.2; (2) take a sample to detect the content of mechanical impurities in the waste oil, mix the waste oil with water at a volume ratio of 3:1, then heat the oil-water mixture to 37°C and keep the temperature constant, and obtain in step (1) The secondary culture solution is added in the oil...

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Abstract

The invention provides a method for removing mechanical impurities in the dirty oil with microorganism, solving the problem of undesirable effect for removing the mechanical impurities in the dirty oil in the existing method for purifying the dirty oil. The method comprises the steps of: (1) inoculating acinetobacter johnsonii into a liquid LB culture medium to carry out accumulation culture, and controlling the cell concentration of culture solution OD=1.1-1.2; and (2) mixing the dirty oil with water according a certain volume ratio, heating up oil water mixture at 37 DEG C and keeping temperature, adding the culture solution obtained by the step (1) into the oil water mixture according to the inoculating quantity, the volume of which is 2-10% of that of the oil water mixture, simultaneously aerating, heating up at 60 DEG C to 90 DEG C after aerating and keeping temperature for 6-8 hours, and stopping heating up. The method can effectively reduce the content of the mechanical impurities in the dirty oil, thereby preventing the mechanical impurities from forming into deposition in a pipe line or equipment and being capable of guaranteeing the running stability of a joint station.

Description

technical field [0001] The invention belongs to the technical field of oil fields, and in particular relates to a method for treating dirty oil by utilizing microbial technology. Background technique [0002] In the process of oil field production, a large amount of dirty oil will inevitably be produced due to oil well operation and equipment maintenance. In order to eliminate the environmental pollution and waste of resources caused by this, the dirty oil must be recycled. [0003] The existing waste oil recycling method is to first carry out purification treatment to the waste oil, and then pump the purified waste oil into the joint station for secondary treatment. At present, the sewage oil purification treatment method is usually to inject the sewage oil into the sewage oil recovery tank and heat it to 80-90°C to melt it, and some mechanical impurities in the sewage oil will naturally settle to the bottom of the sewage oil recovery tank under the action of gravity, there...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/34C02F3/12
CPCY02W10/10
Inventor 姜岩蔡徇吴迪任南琪
Owner DAQING OILFIELD CO LTD
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