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Composition or composite bacterium agent for treating waste well drilling mud

A compound bacterial agent, drilling mud technology, applied in biological sludge treatment, microorganism-based methods, bacteria, etc., can solve the problems of regional ecological environment impact, difficult degradation of harmful substances, and damage to the soil ecological environment.

Active Publication Date: 2016-08-31
陈五岭
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Aromatic hydrocarbons in base oil and diesel oil have high biological toxicity. If not treated effectively, it will have a significant impact on the regional ecological environment, and it is more difficult to handle than water-based mud.
[0003] Under natural conditions, the above-mentioned various harmful substances are difficult to degrade, seriously endangering the soil ecological environment

Method used

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  • Composition or composite bacterium agent for treating waste well drilling mud
  • Composition or composite bacterium agent for treating waste well drilling mud
  • Composition or composite bacterium agent for treating waste well drilling mud

Examples

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preparation example Construction

[0059] On the other hand, the present application also relates to a preparation method of a composite bacterial agent, comprising:

[0060] Bacillus subtilis (Bacillus subtilis), Pseudomonasstutzeri (Pseudomonasstutzeri) and johnsonii (Acinetobacter johnsonii) are cultured separately; Combining and mixing to obtain the composite microbial agent.

[0061] Optionally, the above culture is solid culture, semi-solid culture or liquid culture.

[0062] In some embodiments, in the preparation method of the compound microbial agent, the weight of the respective cultures of the bacterial strains is used for mixing. When mixing, the Bacillus subtilis culture, the Pseudomonas stutzeri culture and about The parts by weight of Acinetobacter subtilis culture are:

[0063] Bacillus subtilis culture 1~3

[0064] Pseudomonas stutzeri cultures 1-6

[0065] Acinetobacter johnsonii culture 1-6;

[0066] Optionally, the strain culture comprises a strain and a culture medium;

[0067] Option...

Embodiment 1

[0140] Example 1 Treatment of waste oil-based mud from shale gas wells

[0141] Harmless treatment of about 1,000 cubic meters of waste oil-based mud produced by a shale gas well.

[0142] The shale gas well site is located on the edge of the Mu Us Desert, surrounded by sand dunes and a few farmlands. It has been drilled and handed over for nearly a year. The waste liquid from the upper layer of the mud pool has basically evaporated naturally. There is no obvious seepage in the pool. In the air-dried layer of 2-3 cm, the water content of the mixed waste under the air-dried layer is 80-90%, the pH value is 13, and the physical state is viscose. Before the treatment, the ambient temperature was 30°C, and the temperature in the mud pool was 17°C. Add the compound bacterial agent into the waste mud pool and stir it before treatment. During the treatment, the ambient temperature was 12-30°C, and the treatment monitoring time was 30 days. During this period, there were two rain pro...

Embodiment 2

[0166] Example 2 Treatment of oil well waste oil-based mud

[0167] Harmless treatment of about 800 cubic meters of waste oil-based mud produced by an oil well.

[0168] The oil well site is located on the edge of the village, and the surrounding landforms are all plowed farmland. The drilling has just been completed, and the pH value of the bottom mud is 13-14, and the physical state is viscose. 18°C, and the bottom mud is 8-10°C. After stirring, the water content of the waste mud is more than 90%, the pH value is 11-12, the temperature is 15-16°C, and the physical state is dilute colloid. The composite bacterial agent is added into the waste mud pool and then stirred for treatment. During the treatment, the ambient temperature is 16-28°C, and the treatment monitoring time is 35 days.

[0169] 1) Shake flask culture: Inoculate Bacillus subtilis, Pseudomonas stutzeri and Acinetobacter johnsoni into beef extract peptone liquid medium for shake flask culture, all cultured at 2...

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Abstract

The invention provides a composition or composite bacterium agent for treating waste well drilling mud. The invention provides a composition, a composite bacterium agent and application thereof. The composition or the composite bacterium agent comprises bacillus subtilis, pseudomonas stutzeri and acinetobacter johnsonii and is used for treating the waste well drilling mud.

Description

technical field [0001] This application belongs to the technical field of oil and gas field environmental protection. Specifically, the present application relates to a composition or a composite bacterial agent for treating waste drilling mud. Background technique [0002] The waste mud discharged during shale gas drilling is a mixture of crude oil, oil-based mud, and mud additives. Like water-based mud, the mud system contains mineral oil, petroleum sulfonated acid, lime, calcium alkylbenzene sulfonate, calcium chloride, sodium dichromate, caustic soda, sodium hexametaphosphate, sulfomethyl tannin, iron Chromium lignosulfonate, oxidized asphalt, chrome lignite, sulfomethyl phenolic resin, sodium polyacrylate, sulfonated tannin and other organic and inorganic compounds, petroleum pollutants and heavy metal ions and other harmful substances. Different from water-based mud, which uses water as the dispersion medium, oil-based mud uses oil as the dispersion medium. It is a c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F11/02C12R1/125C12R1/38C12R1/01
CPCC02F11/02C12N1/20
Inventor 陈五岭于烽
Owner 陈五岭
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