Method for improving breeding speed of water body purifying bacterial colonies
A technology for purifying water bodies and colonies, applied in microorganism-based methods, biochemical equipment and methods, and adding compounds to stimulate growth, etc., can solve the problems of water pollution, slow reproduction, and short growth period, and improve the purification speed. , Improve the purification effect, prolong the effect of the proliferative period
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[0031] In an embodiment of the present invention, the preparation method of the activated medium comprises the following steps:
[0032] a) Soak ginkgo leaves, grape seeds, saponins and sunflower seeds in water for 2 to 3 hours, heat to 80 to 100° C. for 2 to 3 hours, and filter after cooling to obtain a filtrate;
[0033] b) Concentrate the filtrate obtained in step a), and mix it with glucose, agar and beef broth after concentration.
[0034] It should be noted that the mass ratio of glucose, agar, beef broth, ginkgo biloba, grape seeds, saponin rice, sunflower seeds to water is (2~3):(1~2):(10~12):(4 ~6): (2~3): (20~30): (3~4) (120~150).
[0035] It should be noted that the beef broth is a broth medium with a model number of CM0375 produced by Beijing Regen Biotechnology Co., Ltd.
[0036] Wherein the mass ratio of the filtrate volume concentrated in step b) to water is 1: (12-15).
[0037] It should be noted that the activation medium can promote Acinetobacter Johnson, ...
Embodiment 1
[0049] The method for improving the speed of bacterial colony propagation in purified water comprises the following steps:
[0050] a) respectively inserting the microbial strains into the activated culture medium after high temperature and high pressure sterilization, and culturing at 25°C;
[0051] b) respectively adjust the counts of each bacterial solution obtained in step a) and then mix them, and insert the mixed bacterial solution into the breeding nutrient solution under aseptic conditions, and cultivate at room temperature;
[0052] Microbial strains include Acinetobacter johnsonii, Saccharomyces cerevisiae, Raoultella, Pseudomonas mendoza and actinomycetes with a ratio of viable bacteria of 0.35:6:1.3:0.65:0.12; The preservation number is CCTCCM2014023, the preservation number of Saccharomyces cerevisiae is CGMCC12927, the preservation number of Raoultella is CGMCC No.12188, the preservation number of Pseudomonas mendoza is CGMCC 1.15625, and the preservation number ...
Embodiment 2
[0065] The method for improving the speed of bacterial colony propagation in purified water comprises the following steps:
[0066] a) respectively inserting the microbial strains into the activated culture medium after high temperature and high pressure sterilization, and culturing at 20°C;
[0067] b) respectively adjust the counts of each bacterial solution obtained in step a) and then mix them, and insert the mixed bacterial solution into the breeding nutrient solution under aseptic conditions, and cultivate at room temperature;
[0068] Microbial strains include Acinetobacter johnsonii, strains of Saccharomyces cerevisiae, Raoultella, Pseudomonas mendoza and actinomycetes with a live bacterial ratio of 0.5:7:1:0.7:0.2; The preservation number is CCTCC M2014023, the preservation number of Saccharomyces cerevisiae is CGMCC12927, the preservation number of Raoultella is CGMCC NO.12188, the preservation number of Pseudomonas mendoza is CGMCC 1.15625, and the preservation numb...
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