Method for improving breeding speed of water body purifying bacterial colonies

A technology for purifying water bodies and colonies, applied in microorganism-based methods, biochemical equipment and methods, and adding compounds to stimulate growth, etc., can solve the problems of water pollution, slow reproduction, and short growth period, and improve the purification speed. , Improve the purification effect, prolong the effect of the proliferative period

Inactive Publication Date: 2019-08-09
黄山市益天士生物科技有限公司
View PDF12 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Physical methods have remarkable effects, but require a lot of labor, take a long time to take effect, are more complicated to use, and have higher costs; although chemical drugs have quick effects, they are costly, have relatively large toxic and side effects, and have certain residues in water bodies. Water bodies and the surrounding ecological environment will cause pollution; biological methods have less toxic side effects and are conducive to restoring the ecological environment, so they have attracted people's attention
However, the bacterial strains currently used in water purification are difficult to purify water with various pollutants due to their short proliferation period and slow reproduction speed.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for improving breeding speed of water body purifying bacterial colonies
  • Method for improving breeding speed of water body purifying bacterial colonies

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0031] In an embodiment of the present invention, the preparation method of the activated medium comprises the following steps:

[0032] a) Soak ginkgo leaves, grape seeds, saponins and sunflower seeds in water for 2 to 3 hours, heat to 80 to 100° C. for 2 to 3 hours, and filter after cooling to obtain a filtrate;

[0033] b) Concentrate the filtrate obtained in step a), and mix it with glucose, agar and beef broth after concentration.

[0034] It should be noted that the mass ratio of glucose, agar, beef broth, ginkgo biloba, grape seeds, saponin rice, sunflower seeds to water is (2~3):(1~2):(10~12):(4 ~6): (2~3): (20~30): (3~4) (120~150).

[0035] It should be noted that the beef broth is a broth medium with a model number of CM0375 produced by Beijing Regen Biotechnology Co., Ltd.

[0036] Wherein the mass ratio of the filtrate volume concentrated in step b) to water is 1: (12-15).

[0037] It should be noted that the activation medium can promote Acinetobacter Johnson, ...

Embodiment 1

[0049] The method for improving the speed of bacterial colony propagation in purified water comprises the following steps:

[0050] a) respectively inserting the microbial strains into the activated culture medium after high temperature and high pressure sterilization, and culturing at 25°C;

[0051] b) respectively adjust the counts of each bacterial solution obtained in step a) and then mix them, and insert the mixed bacterial solution into the breeding nutrient solution under aseptic conditions, and cultivate at room temperature;

[0052] Microbial strains include Acinetobacter johnsonii, Saccharomyces cerevisiae, Raoultella, Pseudomonas mendoza and actinomycetes with a ratio of viable bacteria of 0.35:6:1.3:0.65:0.12; The preservation number is CCTCCM2014023, the preservation number of Saccharomyces cerevisiae is CGMCC12927, the preservation number of Raoultella is CGMCC No.12188, the preservation number of Pseudomonas mendoza is CGMCC 1.15625, and the preservation number ...

Embodiment 2

[0065] The method for improving the speed of bacterial colony propagation in purified water comprises the following steps:

[0066] a) respectively inserting the microbial strains into the activated culture medium after high temperature and high pressure sterilization, and culturing at 20°C;

[0067] b) respectively adjust the counts of each bacterial solution obtained in step a) and then mix them, and insert the mixed bacterial solution into the breeding nutrient solution under aseptic conditions, and cultivate at room temperature;

[0068] Microbial strains include Acinetobacter johnsonii, strains of Saccharomyces cerevisiae, Raoultella, Pseudomonas mendoza and actinomycetes with a live bacterial ratio of 0.5:7:1:0.7:0.2; The preservation number is CCTCC M2014023, the preservation number of Saccharomyces cerevisiae is CGMCC12927, the preservation number of Raoultella is CGMCC NO.12188, the preservation number of Pseudomonas mendoza is CGMCC 1.15625, and the preservation numb...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a method for improving the breeding speed of water body purifying bacterial colonies. Acinetobacter johnsonii, a saccharomyces cerevisiae strain, raoultella, pseudomonas mendocina and actinomycetes can remove COD, total phosphorus, total nitrogen, heavy metals and other pollutants in a water body, so that the water body purifying effect is improved, wherein an activation culture medium can be used for activating the acinetobacter johnsonii, the saccharomyces cerevisiae strain, the raoultella, the pseudomonas mendocina and the actinomycetes in a dormant state and promoting propagation; a propagation nutrient solution is beneficial to the acinetobacter johnsonii, the saccharomyces cerevisiae strain, the raoultella, the pseudomonas mendocina and the actinomycetes, prolongs the proliferative stage and improves the water body purifying speed. The method can promote breeding of the water body purifying bacterial colonies, the proliferative stage of the bacterial colonies can be prolonged, and purification of the water body can be accelerated.

Description

technical field [0001] The invention relates to the field of colony propagation, in particular to a method for increasing the colony propagation speed in purified water. Background technique [0002] With the development of the national economy and the improvement of people's living standards, as well as the development of cities and industries in my country in recent years, the extensive use of pesticides and fertilizers, the destruction of vegetation, the erosion of soil and water into the lake, etc., the nutrients discharged into the water body continue to increase, making the water body The concentration of oxygen-consuming substances such as ammonia, nitrogen, phosphorus, and organic pollutants in the water increases sharply, resulting in the extinction of fish and shrimps in the water body, enriching nutrients, and finally deteriorating water quality, black odor, seriously destroying the ecological balance system of the water body, and causing the loss of river channels....

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20C12N1/18C12N1/38C12R1/865C12R1/38C12R1/01
CPCC12N1/18C12N1/20C12N1/38
Inventor 汪军陈庆裕
Owner 黄山市益天士生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap