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Microbial preparation for low-temperature soil repair and preparation method and application thereof

A composite microbial inoculum and soil remediation technology, applied in the field of soil remediation, can solve the problems of single treatment effect, unstable enzyme activity, and inability to remediate soil, and is suitable for large-scale production, simple preparation method, and solves the problem of environmental pollution. Effect

Active Publication Date: 2018-11-02
安徽中富恒源生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology uses two different types of bacterium called anoxyredases (or enzymes) together with other substances like yeast or molded sugar to improve degrading pesticidal compounds such as tetrapyrrole carboxamine salt used on crops during growing conditions at temperatures below freezing. By combining these components into one biomass, this new composition becomes more effective when it comes across difficult environments where traditional methods are less efficient. It provides technical advantages over existing technologies due to its ability to efficiently remove both chemicals from contaminated sites while minimizing damage caused by high temperature environment.

Problems solved by technology

This patents discuss various methods used to reduce environmental contamination caused by harmful compounds found naturally occurring at lower temperatures (below 0°C). These techniques include physical removal, bioremediation, and bioaugmentative processes like ferrocene production. Chemotherapy with herbal medicine remains effective against some types of cancer due to its ability to target specific parts of cells involved during metabolism. Microbial agents offer potential solutions towards these issues.

Method used

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  • Microbial preparation for low-temperature soil repair and preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0024] Immobilization of spiny root peroxidase: Mesoporous molecular sieve material SBA-15 was added to spiny root peroxidase with RZ value of 3.2, at a temperature of 5℃; pH of 5.0; rotation speed of 220rpm; immobilization time Obtained after immobilization for 12h.

[0025] Immobilization of soil polyphenol oxidase: Mesoporous molecular sieve material SBA-15 was added to soil polyphenol oxidase at a temperature of 5℃; pH of 7; rotation speed of 220rpm; immobilization time of 12h after immobilization obtain.

[0026] Preparation of the cold-tolerant bacterium Pseudopsis rosea HD1031: cultivate the cold-tolerant bacterium Pseudopsis rosea HD1031 to the logarithmic phase, and then inoculate the cultured species to the logarithmic phase at an inoculum of 2% of the medium volume Into the medium, the medium is a medium with wheat straw powder and sweet potato vine powder as carbon sources, ammonium sulfate and peptone as nitrogen sources, shake 170 rpm on a 10°C shaker, and cultivate ...

Embodiment 2

[0032] Immobilization of spiny root peroxidase: Mesoporous molecular sieve material SBA-15 was added to spiny root peroxidase with an RZ value of 3.2 at a temperature of 20°C; pH of 5.0; rotation speed of 150rpm; immobilization time: Obtained after immobilization in 24h.

[0033] Immobilization of soil polyphenol oxidase: mesoporous molecular sieve material SBA-15 was added to soil polyphenol oxidase at a temperature of 20℃; pH of 10; rotation speed of 150rpm; immobilization time of 24h after immobilization obtain.

[0034] Preparation of the cold-tolerant bacterium Pseudopsis rosea HD1031: cultivate the cold-tolerant bacterium Pseudopsis rosea HD1031 to the logarithmic phase, and then inoculate the cultured species to the logarithmic phase at 5% of the medium volume Into the medium, the medium is a medium with wheat straw powder and sweet potato vine powder as carbon sources, ammonium sulfate and peptone as nitrogen sources, 20°C shaker at 150 rpm, cultured for 48 hours, and the ...

Embodiment 3

[0040] Immobilization of spiny root peroxidase: mesoporous molecular sieve material SBA-15 was added to spiny root peroxidase with an RZ value of 3.2 at a temperature of 12°C; pH of 5.0; rotation speed of 180rpm; immobilization time Obtained after immobilization at 18h.

[0041] Immobilization of soil polyphenol oxidase: mesoporous molecular sieve material SBA-15 was added to soil polyphenol oxidase at a temperature of 13℃; pH of 8; rotation speed of 180rpm; immobilization time of 18h after immobilization obtain.

[0042] Preparation of the cold-tolerant bacterium Pseudopsis rosea HD1031 inoculum: The cold-tolerant bacterium Pseudopsis rosea HD1031 is cultured to the logarithmic phase, and then the cultured species is inoculated to the logarithmic phase at an inoculum of 3% of the medium volume Into the medium, the medium is a medium using wheat straw powder and sweet potato vine powder as carbon sources, ammonium sulfate and peptone as nitrogen sources, at 15°C, shake 160 rpm in ...

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Abstract

The invention discloses a microbial preparation for low-temperature soil repair. The compound microbial preparation comprises a cold-resistant pseudogymnoascus roseus HD1031 bactericide, an aerobic denitrifying cold-resistant acinetobacter johnsonii DBP-3 bactericide and immobilized oxidordeuctase, wherein the immobilized oxidordeuctase consists of immobilized horseradish peroxidase and immobilized soil polyphenol oxidase. The invention further discloses a preparation method and application of the microbial preparation. The microbial preparation for low-temperature soil repair has a removal rate of 86% or more of a pentazolol pesticide and an organophosphorus pesticide and a removal rate of 85% or more of ammonia nitrogen, which are much higher than removal rates in the prior art and a comparison example of the present invention. Thus, the microbial preparation for low-temperature soil repair can effectively repair polluted soil. The preparation method is simple and is suitable for large-scale production. Benefits are brought for remote low-temperature regions. The problem of environmental pollution that urgently needs to be solved is solved.

Description

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Claims

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Application Information

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Owner 安徽中富恒源生物科技有限公司
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