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Acinetobacter johnsonii strain for biological denitrification under low temperature and application thereof

A technology of Acinetobacter johnsonii and microbial strains, applied in the field of biological denitrification, can solve the problems such as the inability to effectively remove nitrate nitrogen and nitrite nitrogen, slow growth of autotrophic nitrifying bacteria, and difficulty in reaching the standard of total nitrogen in effluent, etc. Saving capital construction and operating costs, huge economic and environmental benefits, and effective removal

Active Publication Date: 2012-09-12
北京大学科技开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Researchers have tried to add autotrophic nitrification sludge to the biological treatment tank to improve the removal efficiency of ammonia nitrogen, but it is difficult to carry out denitrification under the conditions of low temperature and high dissolved oxygen, resulting in the nitrate nitrogen and nitrite produced by nitrification reaction. Nitrogen cannot be effectively removed, and the total nitrogen in the effluent is still difficult to meet the standard
In addition, due to the slow growth of autotrophic nitrifying bacteria, it is difficult to persist in the system, and continuous dosing is often required, which increases operating costs

Method used

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  • Acinetobacter johnsonii strain for biological denitrification under low temperature and application thereof
  • Acinetobacter johnsonii strain for biological denitrification under low temperature and application thereof
  • Acinetobacter johnsonii strain for biological denitrification under low temperature and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1. Screening of bacterial strain HA7 with low-temperature heterotrophic nitrification-aerobic denitrification performance

[0038] Specific steps are as follows:

[0039] 1) Take 10ml of sludge that has been domesticated and matured under low temperature conditions into a conical flask filled with 90ml of sterile water and sterilized glass beads, and vibrate on a shaker for 2 hours to fully break up the sludge;

[0040] 2) The crushed sludge was diluted to 10 by the ratio dilution method -1 、10 -2 ,..., 10- 6 For the bacterial suspension of each gradient, take the dilution as 10 -4 、10 -5 、10 -6 Each 0.1mL of the bacterial suspension was evenly spread on the medium containing BTB (sodium acetate per L: 2.73g, NH 4 Cl: 0.306g, KNO 3 : 1.0g, KH 2 PO 4 : 0.15g, MgSO 4 ·7H 2 O: 0.1g, FeSO 4 ·7H 2 O: 0.001g, L-aspartine: 1.0g, BTB (1% soluble in alcohol): 1ml, agar: 16-18g, pH: 7.0-7.3, steam sterilized at 121°C for 20min) on a 10ml solid plate;

[0041...

Embodiment 2

[0048] Example 2. Denitrification experiment of bacterial strains under low temperature conditions

[0049] 1. Biological denitrification experiment using ammonia nitrogen as nitrogen source

[0050] With sodium acetate as the organic carbon source and ammonia nitrogen as the nitrogen source, the removal ability of the bacterial strain HA7 described in Example 1 to ammonia nitrogen was determined. The specific implementation steps are as follows:

[0051] Bacterial strain HA7 is inoculated in the CMa medium of 150ml (every liter contains sodium acetate: 2.73g, NH 4 Cl: 0.306g, KH 2 PO 4 : 0.15g, MgSO 4 ·7H 2 O: 0.1g, FeSO 4 ·7H 2 O: 0.001g, pH: 7.0-7.3, steam sterilization at 121°C for 20 minutes), pre-cultivate on a shaker at 10°C, 160r / min. When the strain grows to the late logarithmic phase, take 10 ml of the bacterial liquid and put it into fresh 150 ml of CMa medium, and carry out shaking culture at 10°C and 160r / min. The reaction solution was taken at regular in...

Embodiment 3

[0057] Example 3. Denitrification experiments of bacterial strains under different temperature conditions

[0058] With sodium acetate as the organic carbon source and ammonia nitrogen as the nitrogen source, the removal ability of the bacterial strain HA7 described in Example 1 to ammonia nitrogen was measured at different temperatures. The specific implementation steps are as follows:

[0059] The strain HA7 was inoculated in 150 ml of CMa medium, and pre-cultured on a shaker at 10°C, 20°C, 30°C, 160r / min. When the strain grows to the late logarithmic phase, take 10ml of the bacterial liquid and put it into fresh 150ml CMa medium, and carry out shaking culture at 10°C, 20°C, 30°C and 160r / min respectively. The reaction solution was taken at regular intervals, and the optical density (OD) of the bacteria was measured. 600 ), centrifuge at 8000rpm for 10min, and take the supernatant to measure the concentration of various nitrogen-containing compounds.

[0060] The result i...

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Abstract

The invention discloses an acinetobacter johnsonii strain capable of carrying out biological denitrification under a low temperature and an application thereof. The acinetobacter johnsonii strain HA7 provided by the invention was collected in the China General Microbiological Culture Collection Center (CGMCC) on September 15, 2010, with the collection number of CGMCC No.4168. At the temperature of 10-20 DEG C, the strain is capable of carrying out biological denitrification as long as the strain is put into the nitrogenous wastewater with dissolved oxygen of 2-6mg / L. The strain has the following beneficial effects: the strain has strong tolerance to low temperature, can grow well under a low temperature and simultaneously has the capabilities of heterotrophic nitrification and aerobic denitrification; and the strain can carry out nitrification and denitrification synchronously under low temperature and aerobic conditions to efficiently and thoroughly remove the total nitrogen in the sewage, thus effectively solving the great difficulty in carrying out biological denitrification under a low temperature and having a good application prospect.

Description

technical field [0001] The invention relates to the field of biological denitrification, in particular to an Acinetobacter johnsonii strain used for low-temperature biological denitrification and its application. Background technique [0002] At present, the situation of water pollution in my country is very serious, and the problem of eutrophication caused by nitrogen and phosphorus pollution is particularly prominent. According to the monitoring results of the environmental department, the water quality of more than half of the seven major river systems in my country is polluted, 1 / 3 of the water bodies in the country are not suitable for fish survival, 1 / 4 of the water bodies are not suitable for irrigation, and 90% of urban waters are seriously polluted. 50% of urban water sources do not meet drinking water standards, 40% of water sources are no longer drinkable, and the main pollutants are ammonia nitrogen and COD Mn , BOD, volatile phenols and petroleum substances. Th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34
Inventor 倪晋仁姚硕
Owner 北京大学科技开发有限公司
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