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Method for processing emulsified oil in soybean oil waste water

A technology of soybean oil and emulsified oil, which is applied in the field of microbial technology and environmental engineering, can solve the problems of difficult biodegradation, large dosage, environmental pollution, etc., and achieve the effect of low energy consumption

Inactive Publication Date: 2016-07-20
NORTHEAST DIANLI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing demulsification technology is mainly to add chemical demulsifiers, but it has the disadvantages of strong selectivity, large dosage, and difficult biodegradation, and often causes serious pollution to the environment during use

Method used

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  • Method for processing emulsified oil in soybean oil waste water
  • Method for processing emulsified oil in soybean oil waste water
  • Method for processing emulsified oil in soybean oil waste water

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0019] (1) Preparation of Acinetobacter calcium acetate bacterial suspension:

[0020] Inoculate a single colony of Acinetobacter calcoaceticus (Acinetobacter calcoaceticus) preserved on the slant with the strain number CGMCCNO: 1.2906 into 100mL sterile seed medium, wherein each group of Acinetobacter calcoaceticus seed medium The dosages are: yeast extract powder 3g / L; peptone 8g / L; NaCl 6g / L; pH=7.5; cultured on a shaker at 30°C at 180rpm for 1 day to obtain a cultured Acinetobacter calcoacetate suspension. The obtained bacterial suspension was stored in a 4°C refrigerator for later use;

[0021] (2) Preparation of microbial flocculant by Acinetobacter johnsonii:

[0022] A single colony of Acinetobacter johnsonii (Acinetobacter johnsonii) preserved on the slant with the strain number CGMCCNO: 1.8030 was inserted into 100mL sterile fermentation medium, and in the fermentation medium of Acinetobacter johnsonii The dosage of each component is respectively: glucose 15.0g / L; ...

Embodiment 1

[0030] Example 1 (Flocculation and removal of emulsified oil in soybean oil wastewater by simply using the microbial flocculant produced by Acinetobacter johnsonii):

[0031] (1) Preparation of microbial flocculant by Acinetobacter johnsonii

[0032] A single colony of Acinetobacter johnsonii (Acinetobacter johnsonii) preserved on the slant with the strain number CGMCCNO: 1.8030 was inserted into 100mL sterile fermentation medium, and in the fermentation medium of Acinetobacter johnsonii The dosage of each component is respectively: glucose 15.0g / L; K 2 HPO 4 3g / L; KH 2 PO 4 3g / L; urea 0.3g / L; yeast extract 0.3g / L; MgSO 4 ·7H 2 O0.1g / L; NaCl0.1g / L; pH=7.5; cultivated on a shaker at 30°C at 150rpm for 5 days to obtain a cultured Acinetobacter johnsonii microbial flocculant; after cooling to room temperature, store the microbial flocculant Store in a 4°C refrigerator for later use;

[0033] (2) Acinetobacter johnsoni with the strain number CGMCCNO: 1.8030 treats the emuls...

Embodiment 2

[0038] Example 2 (Use Acinetobacter calcium acetate to demulsify the emulsified oil in soybean oil wastewater, and then use the inorganic flocculant polyaluminum sulfate to remove the emulsified oil after demulsification)

[0039] (1) Preparation of Acinetobacter calcoacetate bacterial suspension

[0040] Inoculate a single colony of Acinetobacter calcoaceticus (Acinetobacter calcoaceticus) preserved on the slant with the strain number CGMCCNO: 1.2906 into 100 mL of sterile seed medium, and in the seed medium of Acinetobacter calcoaceticus The dosage of each component is: yeast extract powder 3g / L; peptone 8g / L; NaCl 6g / L; pH=7.5; culture on a shaker at 30°C at 180rpm for 1 day to obtain a cultured Acinetobacter calcoacetate suspension , Store the obtained bacterial suspension in a 4°C refrigerator for future use;

[0041] (2) Acinetobacter calcoaceticus with the strain number CGMCCNO: 1.2906 demulsifies the emulsified oil in soybean oil wastewater

[0042] Take 15mL of the ...

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Abstract

The invention aims to provide a method for processing emulsified oil in soybean oil waste water, and belongs to the technical field of microbial technology and environmental engineering. According to the method provided by the invention, acinetobacter calcoaceticus is used for performing emulsion-breaking treatment on the emulsified oil in the soybean oil waste water; then acinetobacter johnsonii is used for producing a microbial flocculant for performing flocculation treatment on the emulsion-broken emulsified oil to remove the emulsified oil in the soybean oil waste water. The method comprises the following steps: (1) preparing a bacterial suspension of acinetobacter calcoaceticus; (2) preparing the microbial flocculant with acinetobacter johnsonii; (3) performing emulsion-breaking treatment on the emulsified oil in the soybean oil waste water; (4) processing the emulsion-broken emulsified oil. The method provided by the invention has a high emulsified oil emulsion-breaking rate kept at 95.39%-96.70%; the microbial flocculant produced by the acinetobacter johnsonii is used for flocculation removal of the emulsion-broken emulsified oil, so as to achieve a higher removal rate of the emulsified oil in the soybean oil waste water, kept at 92.01%-94.45%, and also achieve a higher removal rate of COD in the soybean oil waste water, kept at 47.32%-50.88%.

Description

technical field [0001] The invention belongs to the technical field of microorganism technology and environmental engineering. Background technique [0002] Soybean oil wastewater mainly includes alkali refining wastewater in the refining workshop, centrifuge flushing water, condenser drainage in the leaching workshop and other wastewater discharged from processing processes. This type of wastewater is characterized by high content of oil and organic matter. If it is discharged directly without treatment, it will cause serious water pollution. [0003] Domestic soybean oil wastewater treatment mainly adopts oil separation treatment, coagulation or air flotation treatment, biological treatment and so on. Among them, biological treatment is the core process of soybean oil wastewater treatment, and its main function is to remove organic matter in wastewater; oil separation treatment, coagulation or air flotation treatment are pretreatment processes, and its main function is to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C12N1/20C12R1/01
CPCC02F3/34C12N1/20
Inventor 乔楠樊雪于大禹施云芬魏群王小宁高明星吕迪
Owner NORTHEAST DIANLI UNIVERSITY
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