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Degrading strain for bifenthrin insecticides and application thereof

A bifenthrin and insecticide technology, applied in the fields of environmental microorganisms and agriculture, can solve problems such as endangering ecological balance, human and animal health, environmental problems, and less research, achieving significant degradation effects and enriching the germplasm resource bank Effect

Pending Publication Date: 2021-11-02
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the wide application of pyrethroid pesticides has not only caused serious environmental problems; but also seriously endangered the ecological balance and the health of humans and animals.
At present, the microbial degradation of pyrethroid pesticides mainly focuses on fenpropathrin, cypermethrin and other varieties, but there are few studies on the microbial degradation of bifenthrin, and there are no reports on bifenthrin degradation genes.

Method used

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  • Degrading strain for bifenthrin insecticides and application thereof
  • Degrading strain for bifenthrin insecticides and application thereof
  • Degrading strain for bifenthrin insecticides and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Isolation, screening and identification of degrading bacterial strain F2

[0032] 1. Enrichment culture, isolation and screening of bifenthrin-like insecticide-degrading bacteria:

[0033] 1.1 Enrichment culture of strains

[0034] The 2 parts of soil collected from the pesticide factory were domesticated for 40 days, and the culture solution obtained from the domestication was diluted to 10 -7 , and then spread them evenly on LB agar plates, culture at a constant temperature of 30°C for 5 days, pick single colonies with different colony shapes and colony colors, and streak culture again; repeat streak culture for more than five times, and obtain 13 different strains , numbered F2-13 in sequence, for the next experiment.

[0035] 1.2 Screening of strains

[0036] 1.2.1 Bifenthrin standard curve

[0037] Prepare 100mg / L bifenthrin mother solution with acetone, then dilute the mother solution with acetonitrile to form a series of standard sample solutions (0...

Embodiment 2

[0064] Degradability determination of embodiment 2 bacterial strain F2 to bifenthrin insecticides

[0065] 2.1 Optimization of strain F2 degradation conditions

[0066] Using RSM, the pH(A 2 ), temperature (B 2 ) and inoculation volume (C 2 ) as the independent variable, with Y 3 (Bifenthrin degradation rate) is the response value, and is optimized by Box-Behnken design conditions. The results of the experimental design and responses are detailed in Table 6. By responding to the value Y 3 , that is, the degradation rate of bifenthrin is subjected to polynomial regression analysis, and a quadratic response regression model is established to obtain the degradation rate of bifenthrin (Y 3 ) of the quadratic polynomial response surface regression equation:

[0067] Y 3 =-14.9042+0.8173A 2 +0.7856B 2 +4.9663C 2 +2.96A 2 B 2 -0.0577A 2 C 2 -0.0142B 2 C 2 -0.061A 2 2 -0.0136B 2 2 -4.0235C 2 2

[0068] The variance analysis of the fitted model is shown in Table...

Embodiment 3

[0088] Example 3 Soil remediation experiment of Acinetobacter schindleri (Acinetobacter schindleri) strain F2

[0089] 1. Soil sample for test

[0090] The topsoil (0-20cm) of the farmland was taken from the experimental field of the teaching farm of South China Agricultural University.

[0091] After the soil samples were retrieved, they were first placed in a cool and ventilated place to dry naturally, then ground after air drying, passed through a 2mm sieve, and a certain amount of bifenthrin was dissolved in acetone, and then soaked in diatomaceous earth to completely dissolve the bifenthrin. adsorption.

[0092] The soaked diatomaceous earth is placed in a fume hood to dry, and it is mixed into the soil so that the concentration of bifenthrin in the soil is about 50 mg / kg. Take 500g of each soil sample and cultivate it in a constant temperature and humidity incubator at 30°C. 7 The inoculation amount of CFU / g was inserted into the F2 degrading bacterial agent, and the ...

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Abstract

The invention discloses a degrading strain for bifenthrin insecticides and application thereof. The present invention relates to a method for producing a bacterial strain of Acinetobacter johnsonii, which is a strain F2 of Acinetobacter johnsonii. The strain can effectively degrade bifenthrin and other bifenthrin insecticides in a short time, has a remarkable biodegradation effect, can be used for repairing water bodies, soil and other natural environments polluted by the bifenthrin and other bifenthrin insecticides, can be directly applied to reduce the residual quantity of the bifenthrin insecticides in the water bodies and the soil to 85.84%, the problems of excessive residues of bifenthrin insecticides and environmental pollution in agricultural production are solved, and the method has a wide application prospect.

Description

technical field [0001] The invention relates to the fields of applied environmental microorganisms and agriculture. More specifically, it relates to a bacterial strain F2 that degrades bifenthrin insecticides and its production and application. Background technique [0002] Biodegradation refers to the process of decomposing toxic macromolecular substances in the environment into low-toxic or even non-toxic small molecular compounds through the action of various microorganisms and their degrading enzymes. The research and application of biodegradation technology is an effective way to eliminate pesticide residues and ensure the safety of agricultural products. Compared with traditional physical and chemical methods, it has the advantages of high efficiency, low cost, and no secondary pollution, and has broad application prospects. [0003] Pyrethroid pesticides have high insecticidal activity and are widely used in my country and even the world for pest control in agricultu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A62D3/02C12R1/01
CPCC12N1/20A62D3/02A62D2101/04A62D2101/22A62D2101/28
Inventor 刘承兰韩艺烨
Owner SOUTH CHINA AGRI UNIV
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