Composite microbial preparation for degrading high-oil-content kitchen waste and preparation method and application thereof

A technology for compounding microorganisms and kitchen waste, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc. Air contact specific surface area and other issues to achieve the effect of reducing odor concentration, rapid decomposition, and reducing the release of NH3 and H2S

Inactive Publication Date: 2021-08-27
ANHUI QINGMING ENVIRONMENTAL PROTECTION TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The above-mentioned technologies all belong to the technology of using traditional microbial strains in the agricultural field to mix a certain proportion and then treat food waste. At present, various composite microbial agents can play a certain role in the treatment of food waste, but with the in-situ treatment of food waste For the application of equipment, especially the promotion of miniaturized food waste treatment equipment, there are still some problems with the current compound microbial preparations: First, in the process of processing high oily food waste, due to the high content of animal fat and vegetable oil, general microbial The degr

Method used

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  • Composite microbial preparation for degrading high-oil-content kitchen waste and preparation method and application thereof
  • Composite microbial preparation for degrading high-oil-content kitchen waste and preparation method and application thereof

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preparation example Construction

[0029] The preparation method of the above-mentioned compound microbial preparation for degrading high-oil-containing kitchen waste specifically includes the following steps:

[0030] S1, activated strains

[0031] Inoculate each original strain of the composite microbial preparation on the solid medium corresponding to each strain for activation culture, in which Acinetobacter johnsonii, Bacillus subtilis and Geobacillus stearothermophilus are inoculated on the LB medium, 30~ Cultivate at 40°C for 24-36 hours; inoculate Candida utilis and Phanerochaete chrysosporium on PDA medium, and culture at 25-30°C for 36-48 hours; activate each strain to obtain each single activated strain;

[0032] S2, primary seed liquid shake flask culture

[0033] Each strain activated in S1 was inoculated into the corresponding Erlenmeyer flask liquid medium for shaking flask culture, in which Acinetobacter johnsonii, Bacillus subtilis and Geobacillus stearothermophilus were inoculated on the LB l...

Embodiment 1

[0051] The compound microbial preparation for degrading food waste with high oil content includes Acinetobacter johnsonii, which accounts for 30% of the total bacterial count in the preparation, Candida utilis, which accounts for 20% of the total bacterial count in the preparation, and Chrysosporium chrysosporium, which accounts for 10% of the total bacterial count in the preparation. Phaneroderma, Bacillus subtilis accounting for 20% of the total number of bacteria in the preparation and Geobacillus stearothermophilus accounting for 20% of the total number of bacteria in the preparation.

Embodiment 2

[0053] The compound microbial preparation for degrading high-oil-containing food waste includes Acinetobacter johnsonii accounting for 40% of the total bacterial count in the preparation, Candida utilis accounting for 15% of the total bacterial count in the preparation, and Chrysosporium chrysosporium accounting for 15% of the total bacterial count in the preparation Phaneroderma, Bacillus subtilis accounting for 15% of the total number of bacteria in the preparation and Geobacillus stearothermophilus accounting for 15% of the total number of bacteria in the preparation.

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Abstract

The invention provides a preparation method of a composite microbial preparation for degrading high-oil-content kitchen waste. The composite microbial preparation comprises acinetobacter johnsonii, candida utilis, phanerochaete chrysosporium, bacillus subtilis and geobacillus stearothermophilus. The method comprises the following steps of S1, inoculating strains subjected to activation culture to a fermentation culture medium according to the mass percent of 5%, and performing fermentation culture, and S2, mixing the thalli cultured in the step S1 to prepare liquid mixed bacteria, mixing the liquid mixed bacteria with a solid adsorption carrier according to a mass ratio of 1: (1.5-2), and carrying out carrier adsorption and drying to obtain a composite microbial preparation. The liquid mixed bacteria comprise 30 to 40 percent of acinetobacter johnsonii, 10 to 20 percent of candida utilis, 10 to 15 percent of phanerochaete chrysosporium, 10 to 20 percent of bacillus subtilis and 10 to 20 percent of geobacillus stearothermophilus. Macromolecular compounds such as animal fat, vegetable fat and cellulose which are not easy to decompose in the kitchen waste can be efficiently and rapidly decomposed, and the degradation rate of protein and starch is maintained.

Description

technical field [0001] The invention relates to the technical field of environmental protection microorganisms, in particular to a composite microbial preparation for degrading high-oil-containing kitchen waste, a preparation method and an application thereof. Background technique [0002] With the improvement of living and production standards and the rapid development of society, the catering industry is booming, but the situation of environmental pollution and energy crisis caused by food waste is becoming increasingly severe. With the widespread development of waste separation actions, management methods for food waste treatment in various places have been introduced one after another, and the society's demand for food waste treatment is getting higher and higher. At the same time as my country's urbanization, the amount of food waste generated is increasing year by year. According to statistics, since 2014, the annual output of food waste in my country has exceeded 60 m...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C12N1/14B09B3/00C05F17/20C05F9/04C12R1/01C12R1/125C12R1/72C12R1/645C12R1/07
CPCC12N1/20C12N1/16C12N1/14B09B3/00C05F17/20C05F9/04Y02W30/40
Inventor 柴化建韩保安凌超赵海泉高大月谢东
Owner ANHUI QINGMING ENVIRONMENTAL PROTECTION TECH CO LTD
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