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Microecological preparation and preparation method thereof

A technology of micro-ecological preparations and slanted strains is applied in the field of composite micro-ecological preparations, which can solve problems such as evaluating the mechanism of action, and achieve the effects of reducing the concentration of ammonia nitrogen and nitrite, and reducing the degree of eutrophication.

Inactive Publication Date: 2017-09-15
NANNING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In terms of application, some single strains are mainly independently developed in China, such as photosynthetic bacteria, Bacillus, Bdellovibrio, etc.; compound preparations are mainly imitated or imported foreign product

Method used

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  • Microecological preparation and preparation method thereof
  • Microecological preparation and preparation method thereof

Examples

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Effect test

Embodiment 1

[0033] A kind of probiotics, prepared by the following steps:

[0034] (1) Incline culture of the original strain: Bacillus subtilis, Acinetobacter johnsonii, Lactobacillus, and yeast in the cryopreservation tube were respectively activated on the slope, and then placed in 90mm petri dishes for purification to obtain subtilis Bacillus slant strains, Acinetobacter johnsonii slant strains, Lactobacillus slant strains, and Saccharomyces slant strains, spare;

[0035] (2) Liquid culture of the original strain: Inoculate the slant strain of Bacillus subtilis in the sterilized liquid medium I, and then cultivate it under anaerobic tungsten light at 29°C for 6 days to obtain the original strain of Bacillus subtilis; The slants of Acinetobacter johnsonii were inoculated in the sterilized liquid medium II, followed by aerobic culture at 27°C for 4 days to obtain the original bacteria liquid of Acinetobacter johnsonii; Bacteria liquid medium III, followed by anaerobic culture at 37°C f...

Embodiment 2

[0052] A kind of probiotics, prepared by the following steps:

[0053] (1) Incline culture of the original strain: Bacillus subtilis, Acinetobacter johnsonii, Lactobacillus, and yeast in the cryopreservation tube were respectively activated on the slope, and then placed in 90mm petri dishes for purification to obtain subtilis Bacillus slant strains, Acinetobacter johnsonii slant strains, Lactobacillus slant strains, and Saccharomyces slant strains, spare;

[0054] (2) Liquid culture of the original strain: Inoculate the slant strain of Bacillus subtilis in the sterilized liquid medium I, and then cultivate it under anaerobic tungsten light at 29°C for 6 days to obtain the original strain of Bacillus subtilis; The slants of Acinetobacter johnsonii were inoculated in the sterilized liquid medium II, followed by aerobic culture at 27°C for 4 days to obtain the original bacteria liquid of Acinetobacter johnsonii; Bacteria liquid medium III, followed by anaerobic culture at 37°C f...

Embodiment 3

[0071] A kind of probiotics, prepared by the following steps:

[0072](1) Incline culture of the original strain: Bacillus subtilis, Acinetobacter johnsonii, Lactobacillus, and yeast in the cryopreservation tube were respectively activated on the slope, and then placed in 90mm petri dishes for purification to obtain subtilis Bacillus slant strains, Acinetobacter johnsonii slant strains, Lactobacillus slant strains, and Saccharomyces slant strains, spare;

[0073] (2) Liquid culture of the original strain: Inoculate the slant strain of Bacillus subtilis in the sterilized liquid medium I, and then cultivate it under anaerobic tungsten light at 29°C for 6 days to obtain the original strain of Bacillus subtilis; The slants of Acinetobacter johnsonii were inoculated in the sterilized liquid medium II, followed by aerobic culture at 27°C for 4 days to obtain the original bacteria liquid of Acinetobacter johnsonii; Bacteria liquid medium III, followed by anaerobic culture at 37°C fo...

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Abstract

The present invention relates to the technical field of the preparation of microecological preparations, particularly to a microecological preparation and a preparation method thereof, wherein the raw materials comprise Bacillus subtilis, Acinetobacter johnsonii, lactobacilli and yeast. The preparation method comprises: respectively activating Bacillus subtilis, Acinetobacter johnsonii, lactobacilli and yeast in freezing storage tubes in slopes, and purifying to various slope strains so as to be spare; preparing corresponding raw bacterial liquids from various slope strains; inoculating various raw bacterial liquids onto a sterilized fermentation culture medium I according to a fixed bacterial ratio, and culturing to obtain the primary seed of a composite microecological preparation; inoculating the primary seed onto a sterilized fermentation culture medium II, and culturing to obtain the secondary seed of the composite microecological preparation; and inoculating the secondary seed onto a sterilized fermentation culture medium III, and culturing to obtain the composite microecological preparation finished product. According to the present invention, the composite microecological preparation can effectively reduce nitrogen and phosphorus in the sewage, and can reduce the eutrophication degree of the sewage.

Description

technical field [0001] The invention relates to the field of a composite microecological preparation, in particular to a sewage denitrification and phosphorus removal microecological preparation and a preparation method thereof. Background technique [0002] At present, my country's water environment pollution is quite serious. Among the seven major river systems in the country, about 40% of the water quality is inferior to the fifth-class standard; 75% of the lakes in the country have varying degrees of eutrophication; The nearby sea area is seriously polluted, the frequency of red tides has increased, and the area has expanded. The pollution of water environment has directly threatened the safety of urban drinking water and people's health. Therefore, it is an urgent need for social and economic development and ecological environment construction to restore polluted rivers and lakes. [0003] Eutrophication refers to the phenomenon of water quality pollution caused by the...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C02F3/34C12R1/125C12R1/225C12R1/01
CPCC12N1/20C02F3/341C02F3/347C12N1/16
Inventor 黄福标谭春萍杨远澄周贵金
Owner NANNING UNIV
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