Microecologic preparation for inhibiting vibrio in litopenaeus vannamei culture as well as preparation and application

A micro-ecological preparation, the technology of Vannabin, which is applied in the field of micro-ecological preparations and its preparation for inhibiting Vibrio, can solve problems such as drug residues, restricting the development of the shrimp farming industry, and farmers' economic losses

Inactive Publication Date: 2017-06-13
THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
View PDF3 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the high temperature season of spring and summer every year, various acute infectious diseases often break out, such as nerve necrosis virus, iridescent virus, vibrio and other pathogenic bacteria, which cause a large number of deaths of shrimp seedlings and juvenile shrimp, bringing huge economic losses to farmers. , seriously restricting the development of the shrimp farming industry
According to statistics, the ann

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Microecologic preparation for inhibiting vibrio in litopenaeus vannamei culture as well as preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0047] Example 1 Preparation of a microecological preparation

[0048] Cultivation of each component of the microecological preparation:

[0049] Bacillus safensis (Bacillus safensis) 1A07588 accession number CCTCC NO: M2013290 and Bacillus pumilus including Bacillus pumilus 1A08151 accession number CCTCC NO: M2013291 Cultivation of the starting strains of Bacillus saffron and Bacillus pumilus Cultivate in the order of slant, liquid seed, and liquid fermentation. The slant and seed medium are conventional nutrient agar medium or broth medium; fermentation medium formula (W / V): soybean meal powder 3%, starch 1.5%, corn Syrup 1%, glucose 0.5%, (NH 4 )SO 4 0.1%, KH 2 PO 4 0.5%, NaH 2 PO 4 0.5%, MgSO 4 0.05%, MnSO 4 0.05%, fermentation condition is 28~37℃, aeration culture is 36-48h, the number of viable bacteria ≥10 9 CFU / mL;

[0050] Lactobacillus plantarum includes Lactobacillus plantarum 1A07806 deposit number CCTCCNO: M2013293 and Lactobacillus acidophilus PL7 deposit number CC...

Example Embodiment

[0056] Example 2 Application test of the microecological preparation of the present invention in prawn farming 1

[0057] 1. Materials and methods

[0058] 1. Test product: the bacterial solution of the composite microecological preparation of Example 1, with a total viable cell count of more than 1 billion / ml.

[0059] 2. Test prawns: Divide the prawns into 2 groups, each with 250 tails, and each group with 3 replicates.

[0060] 3. Test water body: The test water was taken from Xiamen Baicheng sand filter seawater.

[0061] 4. Test site: The test was carried out in a half-ton glass fiber tank. The test tank is placed in an open outdoor place, randomly placed under natural conditions.

[0062] 5. Test method: The test cycle is 45 days. The test is fed with regular shrimp feed. The test group is fed with 1kg of test product per ton of feed to ensure that the number of viable bacteria per gram of feed is ≥10 6 CFU / mL, no test product was added to the control group. The weight and surviv...

Example Embodiment

[0072] Example 3 Application test of the microecological preparation of the present invention in shrimp breeding

[0073] 1. Test product: the bacterial liquid of the composite microecological preparation of Example 1, with a total number of viable bacteria above 1 billion / ml.

[0074] 2. Test prawns: use the prawns from seedlings to 30 days. Set up 3 experimental pools and 7 control pools.

[0075] 3. Test site: a shrimp farm in Shentu Town, Zhangpu County.

[0076] 4. Test method: In daily breeding, take the compound bacterial agent of Example 1 and sprinkle it evenly in the pool. Use it once every three days, and use 0.5-1L per acre of aquaculture water. Double use when the water quality deteriorates. The control group did not use bacterial agents. At the same time, the compound bacterial agent of Example 1 was diluted with an appropriate amount of water and sprayed evenly on the surface of the feed. The prawns in the experimental pond were fed with the mixture every day. After ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a microecologic preparation for inhibiting vibrio in litopenaeus vannamei culture as well as preparation and application, and relates to aquaculture. The microecologic preparation comprises at least one of bacillus safensis and bacillus pumilus, and at least one of lactobacillus plantarum and lactobacillus plantarum; when the microecologic preparation is in a form of microbial bacteria solution, the preparation method comprises the following step: mixing a bacillus safensis fermented solution, a bacillus pumilus fermented solution, a lactobacillus plantarum fermented solution and a lactobacillus plantarum fermented solution; at least one of vitamin C, fructo-oligosaccharide, mannitol, and the like is added as a protecting agent to the mixture. The microecologic preparation is applicable to the preparation of preparations for promoting the growing of litopenaeus vannamei, improving the immunity and disease resistance of the litopenaeus vannamei, preventing and controlling the diseases of the litopenaeus vannamei, increasing the survival rate, purifying culture water quality, and particularly preventing and controlling intestinal tract thickening, red-leg disease, eye-rot disease and white spot syndrome virus of the litopenaeus vannamei. The microecologic preparation is applicable to preparation of veterinary drugs, feed additives or water purifiers, and the like.

Description

technical field [0001] The invention relates to aquaculture, in particular to a microecological preparation for inhibiting Vibrio in Litopenaeus vannamei culture, a preparation method and application thereof. Background technique [0002] Since 2003, with the promotion of high-density farming mode, my country's shrimp farming industry has entered a period of rapid development. In 2006, the total amount of shrimp farming in my country was 1.24 million tons, accounting for 39.2% of the global farmed shrimp production, and it has remained the world's first for many years . However, the rapid development of intensive and high-density aquaculture has seriously damaged the ecological environment of aquaculture, and also produced a series of negative effects, including waste of feed resources, environmental deterioration, serious diseases, germplasm degradation, drug residues, etc. , especially disease problems are becoming more and more serious. Shrimp white spot virus, taura vir...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20C02F3/34A01K61/59A23K10/18A23K50/80A61K35/747A61P31/04A61P37/04A61K35/742C12R1/07C12R1/25C12R1/23
CPCA61K35/742A61K35/747C02F3/34C12N1/20A61K2300/00Y02A40/81Y02A40/818
Inventor 邵宗泽单大鹏李旭林剑辉王丽萍
Owner THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap