Microecologic preparation for inhibiting vibrio in litopenaeus vannamei culture as well as preparation and application
A micro-ecological preparation, the technology of Vannabin, which is applied in the field of micro-ecological preparations and its preparation for inhibiting Vibrio, can solve problems such as drug residues, restricting the development of the shrimp farming industry, and farmers' economic losses
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Embodiment 1
[0047] Embodiment 1 A kind of preparation of probiotics
[0048] The cultivation of the bacteria of each component in the probiotics:
[0049] Bacillus safensis (Bacillus safensis) 1A07588 deposit number CCTCC NO: M2013290 and Bacillus pumilus including Bacillus pumilus (Bacillus pumilus) 1A08151 deposit number CCTCC NO: M2013291 cultivation: the culture of starting strains Bacillus safensis and Bacillus pumilus Cultivate in the order of slant, liquid seed, and liquid fermentation, and the slant and seed medium are conventional nutrient agar medium or broth medium; fermentation medium formula (W / V): 3% bean cake powder, 1.5% starch, corn Syrup 1%, Glucose 0.5%, (NH 4 ) SO 4 0.1%, KH 2 PO 4 0.5%, NaH 2 PO 4 0.5%, MgSO 4 0.05%, MnSO 4 0.05%, the fermentation condition is 28~37℃, the aeration culture is 36-48h, the number of viable bacteria is ≥10 9 CFU / mL;
[0050] Lactobacillus plantarum includes Lactobacillus plantarum (Lactobacillus plantarum) 1A07806 preservati...
Embodiment 2
[0056] Application test 1 of embodiment 2 probiotics of the present invention in prawn culture
[0057] 1. Materials and methods
[0058] 1. Test product: the compound probiotic preparation bacterium liquid of embodiment 1, the total number of viable bacteria is more than 1 billion / ml.
[0059] 2. Test prawns: divide the prawns into 2 groups, 250 tails in each group, and set 3 repetitions in each group.
[0060] 3. Test water body: The test water is taken from sand-filtered seawater in Baicheng, Xiamen.
[0061] 4. Test site: The test is carried out in a half-ton glass fiber tank. The test cylinders were placed in an open place outdoors and placed randomly under natural conditions.
[0062]5. Test method: The test period is 45 days. The test adopts conventional prawn feed feeding, and the test group adds 1 kg of test product per ton of feed to ensure that the number of viable bacteria in each gram of feed is ≥ 10 6 CFU / mL, no test product was added to the control group. O...
Embodiment 3
[0072] The application test of the microecological preparation of the present invention in the culture of prawn shrimp fry of embodiment 3
[0073] 1. Test product: the compound probiotic preparation bacterium liquid of embodiment 1, the total number of live bacteria is more than 1 billion / ml.
[0074] 2. Experimental prawns: The prawns from seedlings to 30 days old are used throughout. Set up 3 experimental pools and 7 control pools.
[0075] 3. Test site: a prawn farm in Shentu Town, Zhangpu County.
[0076] 4, test method: when raising daily, get embodiment 1 compound bacterial agent pool and evenly splash. It is used once every three days, and the dosage per mu of aquaculture water body is 0.5-1L, and it is doubled when the water quality deteriorates. The control group did not use the bacteria agent. Get the composite bacterial agent of embodiment 1 at the same time and evenly spray on the feed surface after diluting with appropriate amount of water, mix and feed the p...
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