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Acinetobacter johnson aj-3 strain and use thereof

A technology of AJ-3 and bacteria strains, applied in the field of environmental microorganisms, can solve the problems of large amount of chemical sludge, high treatment costs, and large dosage of chemicals, and achieve high phosphorus removal efficiency, strong environmental adaptability, and stable phosphorus removal efficiency Effect

Active Publication Date: 2016-01-20
威海海能海洋生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemical phosphorus removal is mainly a chemical precipitation method, which has the advantages of simple operation, high removal rate, and stable operation; The final disposition of the

Method used

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  • Acinetobacter johnson aj-3 strain and use thereof
  • Acinetobacter johnson aj-3 strain and use thereof
  • Acinetobacter johnson aj-3 strain and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Isolation, Screening and Identification of High-efficiency Phosphorus Accumulating Bacteria Acinetobacter Johnson AJ-3

[0023] (1) Medium

[0024] Beef extract peptone solid medium: beef extract 3g / L, peptone 10g / L, NaCl5g / L, agar 20g / L, pH7.0-7.2 (liquid medium without agar);

[0025] Phosphorus-limited (phosphorus-rich) solid medium: Dissolve 8.372g 3-morpholinopropanesulfonic acid and 0.717g N-tris(hydroxymethyl)methylglycine in 30mL deionized water, adjust the pH to 7.4 with 10mol / L KOH, press Add the solution in the following order: 0.01mL1.830%FeSO 4 Solution (now equipped), 5mL1.9mol / LNH 4 Cl, 1mL0.276mol / LK 2 SO 4 , 0.025mL0.02mol / LCaCl 2 ·2H 2 O, 0.42mL1.25mol / LMgCl 2 ·6H 2 O, 20mL2.5mol / LNaCl, 0.02mL trace element mixture (ammonium heptamolybdate 3×10 -6 mol / L, H 3 BO 3 4×10 -4 mol / L, CoCl 2 3×10 -5 mol / L, CuSO 4 10 -5 mol / L, MnCl 2 8×10 -5 mol / L, ZnSO 4 10 -5 mol / L), 10mL1% glucose, 0.4mL1.686% vitamin B1, 250μL 6.928%K 2 HPO 4 (Add 5mL for rich phospho...

Embodiment 2

[0037] Example 2 Determination of the growth curve of Acinetobacter johnsonii AJ-3 strain

[0038] (1) Strains: AJ-3 strain.

[0039] (2) Medium

[0040] Beef extract peptone solid medium: the formula is the same as in Example 1

[0041] Synthetic sewage liquid culture medium: sodium acetate 0.925g / L, peptone 0.1g / L, yeast extract 0.01g / L, NaCl0.05g / L, KH 2 PO 4 0.0655g / L, MgCl 2 ·6H 2 O0.1412g / L, CaCl 2 0.025g / L, pH7.0-7.2.

[0042] (3) Method

[0043] Take 20μL of glycerol bacteria stored at -20℃ and spread it on the plate of beef extract peptone solid medium. After incubating at 28℃ for 24h, pick a single colony and mix it with 0.3mL sterile water. Take 100μL and spread it on beef extract peptone solid culture. Use a punch (aperture 0.5cm) to take 2 holes into 15mL beef extract peptone liquid medium, place them in a shaker at 28℃, 170r / min for 24h (OD 600 ≈0.9) Activated and used as seed solution; then 8% of the inoculum was inserted into a 250mL Erlenmeyer flask containing 100mL...

Embodiment 3

[0046] Example 3 The influence of the liquid volume on the biological phosphorus removal effect of Acinetobacter johnsonii AJ-3 strain.

[0047] (One) Strain: AJ-3 strain.

[0048] (2) Medium

[0049] Beef extract peptone solid medium: the formula is the same as in Example 1;

[0050] Synthetic sewage liquid culture medium: the formula is the same as in Example 2.

[0051] (3) Method

[0052] Take 20μL of glycerol bacteria stored at -20℃ and spread it on the plate of beef extract peptone solid medium. After incubating at 28℃ for 24h, pick a single colony and mix it with 0.3mL sterile water. Take 100μL and spread it on beef extract peptone solid culture. Use a punch (aperture 0.5cm) to take 2 holes into 15mL beef extract peptone liquid medium, place them in a shaker at 28℃, 170r / min for 24h (OD 600 ≈0.9) activated and used as a seed solution; then 8% of the inoculum was inserted into a 250mL Erlenmeyer flask containing different amounts of synthetic sewage liquid culture medium (the...

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Abstract

The invention belongs to the field of environmental microorganisms ， Specifically involving a strain of Acinetobacter johnsonii ( Acinetobacter ? johnsonii ) The isolation and cultivation of AJ-3 strain and its application in sewage phosphorus removal, which is applied to the biological phosphorus removal of urban sewage treatment plants. Acinetobacter johnsonii described in the present invention ( Acinetobacter ? johnsonii ) AJ-3 strain, the deposit number is: CCTCC? M2014023, the preservation place is: China Center for Type Culture Collection, and the preservation date is: January 16, 2014. The present invention is a strain of Acinetobacter johnsonii ( Acinetobacter ? johnsonii ) AJ-3 strain, which grows fast, has strong environmental adaptability, and has low requirements for dissolved oxygen. It can grow rapidly in the liquid medium of synthetic sewage containing phosphorus and remove phosphorus. The efficiency of phosphorus removal is high and stable, reaching 55 %~60.1%, no secondary pollution. Therefore, the AJ-3 strain can be applied to the treatment of phosphorus-containing sewage, and it is an important microbial resource in the process of biological phosphorus removal from water bodies, and has a good application prospect.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, in particular to a strain of Acinetobacter johnsonii ( Acinetobacter johnsonii ) The isolation and cultivation of AJ-3 strain and its application in sewage phosphorus removal, which is applied to the biological phosphorus removal of urban sewage treatment plants. Background technique [0002] From 2010 to 2012, the Bulletin on the State of the Environment in China showed that the eutrophication of 62 key lakes (reservoirs) controlled by the state was prominent, and the water quality in the coastal waters of the East China Sea was extremely poor and heavily polluted in the past three years, and the main pollution indicators included Phosphorus; and eutrophication will reduce the transparency of water bodies, produce harmful gases and biotoxins, affect the health of humans and animals, destroy biodiversity in water bodies, affect tourism and shipping, etc.; therefore, removing phosphorus...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/10
Inventor 蒋冬花谢祥聪胡优李晓倩
Owner 威海海能海洋生物科技有限公司
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