Acinetobacter johnsonii LP28 and method for preparing low-temperature alkali lipase by using acinetobacter johnsonii

Abstract

The invention relates to acinetobacter johnsonii LP28 and a method for preparing low-temperature alkali lipase by using the acinetobacter johnsonii. The strain is preserved in CGMCC, the preservation number is CGMCC 3069, and the homology of a 16S rDNA sequence of the strain and DQ257426 is as high as 100 percent. The method for preparing the low-temperature alkali lipase comprises the following steps: (1) screening bacterial strains; (2) seed cultivation; (3) fermentation and cultivation; and (4) separation and purification. The acinetobacter johnsonii LP28 is from the soil polluted by oil in a saline and alkaline land of Tianjin Bohai Bay, the enzyme production is stable, the raw material source is wide, and the production cost is low; and the obtained low-temperature alkali lipase has good stability for additive components of a washing agent such as surfactant, oxidant, protease and the like, and can be used as enzyme added into the washing agent to be applied in the industry of the washing agent.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to Acinetobacter johnsonii LP28 and a method for preparing low-temperature alkaline lipase by using the bacteria. Background technique [0002] Lipase (lipase) is called triglyceride hydrolase, which is mainly used to hydrolyze and synthesize triglyceride formed by glycerol and insoluble long-chain fatty acids with more than 12 carbon atoms. It is a special ester bond hydrolase, Esters can only be hydrolyzed at the oil-water interface in heterogeneous systems or in insoluble systems. According to the nomenclature and classification principles of the International Union of Biochemistry, the number of lipase is EC 3.1.1.3, the system name is triacylglycerol acyl hydrolase, and the customary name is lipase. [0003] Lipase hydrolysis of fat has the advantages of high efficiency, mild reaction conditions, and non-toxicity. It does not need coenzymes to catalyze the hydrolysis of triglycer...

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Problems solved by technology

[0007] In addition, the reported low-temperature lipase-producing bacteria are mainly bacteria and a small amount of fungi, and some strains of these bacteria produce low-temperature lipase due to their low optimum enzymatic hydrolysis temperature (<20°C). It is unstable under the conditions, and its application is limited by the same energy consumption as in storage. Therefore, obtaining an excellent low-temperature stable lipase-producing strain has also become the key to research.

[0008]At present, the research work on low-temperature alkaline lipase at home and abroad has just started. The low-temperature lipase studied is mainly produced by cold-tolerant microorganisms, and there are few strains available for research , its performance and suitability for industrialized production methods have not yet had a sound technical system

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