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Acinetobacter johnsonii EAb-1 and application thereof

A technology of Acinetobacter johnsonii, eab-1, applied in application, bacteria, animal repellent, etc., to achieve the effect of good lethal activity and growth promotion

Inactive Publication Date: 2017-10-20
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the effect of existing biocontrol bacteria on controlling root-knot nematodes still needs to be improved, and new biocontrol bacteria also need to continue to be developed

Method used

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  • Acinetobacter johnsonii EAb-1 and application thereof
  • Acinetobacter johnsonii EAb-1 and application thereof
  • Acinetobacter johnsonii EAb-1 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] The screening and identification of embodiment 1 Acinetobacter johnsonii EAb-1

[0015] Eupatorium adenophorum was collected near the chemistry building of Yunnan Agricultural University. The whole plant of Eupatorium adenophorum was collected, washed with clean water, and the roots, stems and leaves were placed in sections. In the ultra-clean workbench, the surface sterilization method was used to remove the bacterial contamination on the surface of the Eupatorium adenophorum plant. It was washed with 30% hydrogen peroxide for 30 seconds, 75% alcohol for 2 minutes, and sterile water for three times.

[0016] 1. Sterilization

[0017] The used petri dishes, centrifuge tubes, test tubes, pipette tips and other experimental equipment and sterile water were sterilized by high pressure at 0.1MPa, 121°C, and sterilized for 30 minutes.

[0018] 2. Grinding

[0019] Put the sterilized Eupatorium adenophorum roots, stems and leaves into sterile mortars and grind them in an ul...

Embodiment 2

[0029] The biological identification of embodiment 2 Acinetobacter johnsonii EAb-1

[0030] The strains were inoculated on LB medium and incubated at 28°C for 3 days.

[0031] 1. Morphological observation:

[0032] After picking a single colony and smearing it on a glass slide, stain the tested strain with crystal violet to observe the morphological characteristics of the bacteria. Escherichia coli was used as a control for Gram staining. Observe the colony characteristics directly on the culture medium.

[0033] The observation results are: the bacterium is rod-shaped, without spores, unable to move, and Gram-negative. The colonies are milky yellow, entire, round and raised, moist. The results obtained were the same as the published Acinetobacter johnsonii, and the strain was judged to be Acinetobacter johnsonii morphologically.

[0034] 2. Molecular biological identification

[0035] Acquisition of bacterial strain DNA: In a sterile environment, select the colony of th...

Embodiment 3

[0047] Embodiment 3 The artificial propagation of Acinetobacter johnsonii EAb-1

[0048] One-stage multiplication culture of EAb-1: use standard LB solid medium, adjust the pH value to 4.3-6.3, invert the plate and wait for cooling, inoculate EAb-1 strain, and cultivate at a temperature of 15°C-28°C for 1-3 days, see A single colony can grow.

[0049] Second-stage propagation medium for EAb-1: standard LB liquid medium, pH 4.3-6.3. Pick a single colony grown after the first step of expansion, insert it into 100mL LB liquid medium, and cultivate it overnight on a shaker at 28°C, then insert the bacterial suspension into the second stage of expansion medium, at a temperature of 15°C~ Cultivate at 28°C for 1 to 3 days, stir once every 12 hours, and inoculate at the ratio of inoculating 400 μL of bacterial suspension with 2L of liquid medium.

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Abstract

The invention discloses an Acinetobacter johnsonii EAb-1 and an application thereof. The Acinetobacter johnsonii EAb-1 is collected in the China General Microbiological Culture Collection Center on May 5, 2017, and the collection number is CGMCC No. 14101. The Acinetobacter johnsonii EAb-1 is applied to heterorhabditis megidis prevention, has excellent killing activity, is applied to promotion of Ageratina adenophora growth and can effectively promote growth of crofton weeds.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to Acinetobacter johnsonii EAb-1 and its application. Background technique [0002] Root-knot nematode is a serious plant nematode parasitic disease, often causing serious economic losses. The control of root-knot nematode disease is an urgent problem to be solved in agricultural production. Traditional control measures mainly include chemical control, crop rotation and use of disease-resistant varieties, but these control measures have their limitations. The use of microorganisms to control root-knot nematodes has the advantage of sustainable development and is one of the ideal ways for sustainable management of root-knot nematode diseases. [0003] Research reports on the use of biocontrol bacteria to control root-knot nematodes mainly include rhizosphere growth-promoting bacteria, Pasteurella puncture, Pseudomonas, Bacillus thuringiensis, Bacillus subtilis, Paenibacillu...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P5/00A01P21/00C12R1/01
CPCA01N63/00C12N1/205C12R2001/01
Inventor 张某兰明先高熹吴国星王扬李召波夏涛鲁武锋李梦月李丽芳李建一查友贵唐萍袁远
Owner YUNNAN AGRICULTURAL UNIVERSITY
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