Acinetobacter johnsonii bacterial strain capable of low-temperature synchronous denitrification phosphorous removal and application thereof

A technology of Acinetobacter johnsoni, nitrogen and phosphorus removal, applied in the direction of bacteria, microorganism-based methods, biological water/sewage treatment, etc., can solve the problem that nitrate nitrogen and nitrite nitrogen cannot be effectively removed, and the total nitrogen in the effluent is difficult to reach the standard , increasing operating costs and other issues, to achieve the effect of saving infrastructure investment and operating costs, broad application prospects, and simplifying the process

Active Publication Date: 2013-12-11
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that adding autotrophic nitrification sludge to the biological treatment tank can improve the removal efficiency of ammonia nitrogen, but it is difficult to carry out denitrification under the conditions of low temperature and high dissolved oxygen, which leads to the inability of nitrate nitrogen and nitrite nitrogen produced by nitrification reaction. has been effectively removed, but the total nitrogen in the effluent is still difficult to reach the standard
On the other hand, the accumulation of oxidized nitrogen has a certain inhibitory effect on the anaerobic release of phosphorus, so that the phosphorus removal effect is usually not guaranteed.
In addition, since autotrophic nitrifying bacteria grow extremely slowly under low temperature conditions, they are easily washed out from the treatment system with the discharge of excess sludge, and continuous dosing is often required, which increases operating costs

Method used

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  • Acinetobacter johnsonii bacterial strain capable of low-temperature synchronous denitrification phosphorous removal and application thereof
  • Acinetobacter johnsonii bacterial strain capable of low-temperature synchronous denitrification phosphorous removal and application thereof
  • Acinetobacter johnsonii bacterial strain capable of low-temperature synchronous denitrification phosphorous removal and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1. Temperature characteristics of bacterial strain HA7

[0034] Investigate the growth performance of the strain under different temperature conditions of 10°C, 15°C, 20°C, 25°C, and 30°C. The specific implementation steps are as follows:

[0035] Inoculate strain HA7 in 150ml of BM medium (containing sodium acetate per liter: 2.73g, NH 4 Cl: 0.306g, KH 2 PO 4 : 0.1g, MgSO 4 ·7H 2 O: 0.1g, FeSO 4 ·7H 2 O: 0.001g, pH: 7.0-7.3, steam sterilized at 121°C for 20 minutes), and pre-cultivated on a shaker at 10°C, 15°C, 20°C, 25°C, 30°C, 160r / min. When the strain grows to the late logarithmic phase, take 10ml of the bacterial liquid and put it into 150ml of fresh BM medium, and carry out shaking culture at 10°C, 15°C, 20°C, 25°C, 30°C, and 160r / min. Take the reaction solution at regular intervals and measure the OD 600 , to characterize the growth of the strain.

[0036] The result is as figure 1 As shown, the strains grow best between 20°C and 30°C, the grow...

Embodiment 2

[0037] Example 2. Removal of Phosphate and Nitrogen by Strain HA7 at 10°C

[0038] Using sodium acetate as the organic carbon source, ammonia nitrogen as the nitrogen source, and phosphate as the phosphorus source, the nitrogen and phosphorus removal capabilities of the strain HA7 were determined. The specific implementation steps are as follows:

[0039] The strain HA7 was inoculated in 150ml of BM medium, and pre-cultivated on a shaker at 10°C and 160r / min. When the strain grows to the late logarithmic growth phase, take 10ml of the bacterial liquid and put it into fresh 150ml BM medium, and carry out shaking culture at 10°C and 160r / min. The reaction solution was taken at regular intervals, and after being centrifuged at 8000 rpm for 10 minutes, the supernatant was taken to measure the concentration of various nitrogen-containing compounds and phosphate.

[0040] The result is as figure 2It can be seen that the concentrations of nitrogen and phosphorus decreased continu...

Embodiment 3

[0041] Example 3. Removal of nitrogen by bacterial strain HA7 at different temperatures

[0042] The denitrification performance of the strain was investigated at different temperatures of 10°C, 15°C, 20°C, 25°C, and 30°C. The specific implementation steps are as follows:

[0043] The strain HA7 was inoculated in 150ml of BM medium, and pre-cultured on a shaker at 10°C, 15°C, 20°C, 25°C, 30°C, 160r / min. When the strain grows to the late logarithmic phase, take 10ml of the bacterial liquid and put it into 150ml of fresh BM medium, and carry out shaking culture at 10°C, 15°C, 20°C, 25°C, 30°C, and 160r / min respectively. The reaction solution was taken at regular intervals, and after being centrifuged at 8000 rpm for 10 minutes, the supernatant was taken to measure the concentration of various nitrogen-containing compounds.

[0044] The result is as image 3 As shown, the strain can effectively remove total nitrogen in the temperature range of 10°C-30°C. At 20°C, the average r...

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PUM

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Abstract

The invention discloses an Acinetobacter johnsonii bacterial strain capable of low-temperature synchronous biological denitrification phosphorous removal and an application. The Acinetobacter johnsonii bacterial strain HA7 is preserved in the ordinary microorganism center of China Committee for Culture Collection of Microorganisms (CGMCC) on September 15th, 2010 and the preservation number is CGMCC No. 4168. The bacterial strain has strong tolerance ability to low temperature, good growth at low temperature condition (10 DEG C) and has abilities of heterotrophic nitrification, aerobic denitrification and aerobic phosphorus uptake. Under the low temperature and aerobic conditions, the bacterial strain enables generation of simultaneous nitrification and denitrification to effectively remove the total nitrogen in waste water and remove phosphorous simultaneously, so that low-temperature synchronous denitrification phosphorous removal for one-step at an aerobic phase can be realized, and the bacterial strain has wide application prospect.

Description

technical field [0001] The invention relates to the field of biological nitrogen and phosphorus removal, in particular to an Acinetobacter johnsonii strain used for biological nitrogen and phosphorus removal under low temperature conditions and its application. Under low temperature conditions, the strain has the function of heterotrophic nitrification-aerobic denitrification, and can remove phosphate in sewage at the same time during the process of simultaneous nitrification and denitrification denitrification, so as to achieve the purpose of simultaneous biological nitrogen and phosphorus removal . Background technique [0002] The eutrophication of water bodies caused by nitrogen and phosphorus pollution poses new challenges to water resource protection and water pollution control in my country. The water quality of more than half of the seven major river systems in my country is polluted, and 90% of the urban waters are seriously polluted. The main pollutants are ammoni...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C02F3/02C12R1/01
CPCY02W10/10
Inventor 倪晋仁姚硕
Owner PEKING UNIV
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