The invention discloses a preparation method of a grapey flavoring essence for tobacco. The preparation method comprises the following steps: (1) preparing raw materials; (2) uniformly mixing propylene glycol, acetic acid, ethyl acetate, ethyl butyrate, 2-methylbutylaldehyde, benzaldehyde, acetophenone, linalool oxide, linalool, rose oxide, dimethyl benzyl carbinol, nerol, geraniol, geranyl propionate, menthyl acetate and beta-ionone; (3) adding imethylbenzylcarbinyl acetate, methyl anthranilate, methyl cinnamate, beta-damascone, nerolyl acetate, geranyl isobutyrate, dihydroactinidiolide and cinnamyl isobutyrate to a material obtained in step 2; 4, adding gamma-decalactone, delta-dodecalactone, Davana oil and glyceryl triacetate to obtain a material obtained in step 3, and uniformly mixing the added substances and the material; and (5) aging a material obtained in step 4. The grapey flavoring essence for tobacco has the sweet flavor of grapes, and also ahs full flavor and unique style.