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Composite microbial agent and application thereof

A technology of compound microbial agent and microbial strain, which is applied in the field of microorganisms, can solve problems such as farmland soil compaction and hardening, social and economic losses, ecological safety and food safety, and achieve abundant and available microbial resources, stable phosphorus-dissolving function, and good promotion. the effect of the effect

Active Publication Date: 2022-01-07
广西绿友农生物科技股份有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the extensive use of chemical fertilizers not only directly causes the compaction and hardening of farmland soil and the structural changes of the bacterial flora in the soil, but also indirectly brings about a series of problems such as ecological safety and food safety, resulting in a large number of social and economic losses and serious environmental pollution.

Method used

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  • Composite microbial agent and application thereof
  • Composite microbial agent and application thereof
  • Composite microbial agent and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] A microbial inoculant comprising the following three strains:

[0043] Micrococcus luteus (Micrococcus luteus-GXMD-hs-11052), on August 12, 2021, with the preservation number GDMCC NO: 61867, was preserved in the Guangdong Microbial Culture Collection Center;

[0044] Bacillus subtilis (Bacillus subtilis-GXMD-hs-13035), with the preservation number GDMCC NO: 61868 on August 12, 2021, was preserved in the Guangdong Provincial Microbial Culture Collection Center;

[0045] Bacillus Paenibacillus Gxun-30, on June 13, 2019, with the preservation number GDMCC NO: 60687, was preserved in the Guangdong Microbial Culture Collection Center;

[0046] The composition by weight of the composite microbial bacterial agent is: 1-2 parts of Micrococcus luteus (Micrococcus luteus-GXMD-hs-11052), 1-2 parts of Bacillus subtilis (Bacillus subtilis-GXMD-hs-13035), fungoid 1-2 copies of Bacillus Paenibacillus Gxun-30. The ratio of the three strains is 1:1:1.

[0047] Application of the abo...

Embodiment 2

[0055] 1. Isolation, purification and identification of strains

[0056] Including the following steps:

[0057] Step 1 material preparation

[0058] Step 1.1 Sample sampling: The soil sampling site is Guangxi Nonggang National Nature Reserve. Remove the topsoil, collect soil samples at a depth of 15cm below the surface, store them in a 4°C incubator, and bring them back to the laboratory for further processing.

[0059] Step 1.2 Separation and purification medium:

[0060] LB liquid medium: tryptone 10.0g, yeast powder 5.0g, sodium chloride 5.0g, distilled water 1000mL.

[0061] LB solid medium: tryptone 10.0g, yeast powder 5.0g, sodium chloride 5.0g, agar powder 15g, distilled water 1000mL.

[0062] PDA medium: 200.0g potato, 20.0g glucose, 20.0g agar, 1000mL distilled water.

[0063] Step 2 Isolation and purification of bacterial strains

[0064] Step 2.1 Separation and Purification

[0065] Add 2g of soil sample to 18ml of sterile water to prepare soil suspension, sh...

experiment example 1

[0069] Single strain growth promotion experiment

[0070] 2.1 Preparation of strain fermentation broth

[0071] Streak the preserved strains on the LB solid medium, pick a single colony into 1ml LB liquid medium for shaking culture overnight, inoculate 1ml of the above bacterial solution into the liquid medium, shake the table (30-32°C, 150-200r / min) After one day of shaking culture, it was used to water the experimental seedlings.

[0072] 2.2 Seedling growth promotion experiment

[0073] Use the seedlings of Citrus aurantii to carry out the experiment, and the specific steps are as follows:

[0074] Slow seedlings: transplant the experimental seedlings of Fructus Aurantii into flower pots filled with soil + substrate (1:1) to ensure that the nutrition of the original soil is relatively consistent.

[0075] Seedling selection: After slowing down the seedlings for a period of time, pick the Fructus aurantii seedlings with relatively consistent growth to carry out the exper...

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Abstract

The invention provides a composite microbial agent and application thereof, and the composite microbial agent comprises micrococcus luteus which is preserved in Guangdong Microbial Culture Collection Center on August 12, 2021 with a preservation number of GDMCC NO: 61867; bacillus subtilis which is preserved in Guangdong Microbial Culture Collection Center on August 12, 2021 with a preservation number of GDMCC NO: 61868; and bacillus pseudomycoides which is preserved in Guangdong Microbial Culture Collection Center on June 13, 2019 with a preservation number of GDMCC NO: 60687. According to the invention, the defects in the prior art are overcome, the design is reasonable, the structure is compact, the microbial agent is easy to separate and culture, and the inventor also establishes a corresponding culture method. Researches show that the composite microbial agent has a good growth promoting effect on crops. The discovery of the strain enriches available microbial resources in China, and the strain has the advantages of stable phosphorus dissolving function, high efficiency and environmental friendliness, and has a good application prospect in the aspect of agricultural planting.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a composite microbial bacterial agent and its application. Background technique [0002] For a long time, chemical fertilizers have played a huge role in increasing crop yields, and the proportion of chemical fertilizers in agricultural inputs is 40%-60%. However, the extensive use of chemical fertilizers not only directly causes the compaction and hardening of farmland soil and the structural changes of the bacterial flora in the soil, but also indirectly brings about a series of problems such as ecological safety and food safety, resulting in a large number of social and economic losses and serious environmental pollution. . [0003] Under the strategic requirements of national agricultural modernization and sustainable development, it is becoming more and more important to develop new microbial fertilizers to replace some chemical fertilizers. As a new type of microbi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01N63/20A01P21/00C12R1/265C12R1/125C12R1/07
CPCC12N1/20A01N63/20A01N63/22
Inventor 姜明国朱英芝黄文善申乃坤卢杰罗春婷钟仪珊
Owner 广西绿友农生物科技股份有限公司
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