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A kind of human mycoplasma pneumoniae culture medium and its diagnostic kit

A technology of mycoplasma pneumoniae and culture medium, applied in the direction of microorganism-based methods, microorganism measurement/inspection, microorganisms, etc., can solve the problems of high cost, easy pollution, and tediousness, and achieve special physiological and biochemical effects, obvious effects, and good selectivity Effect

Active Publication Date: 2017-11-21
众爱生河北生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The traditional methods of isolation, culture and identification of difficult-to-culture bacteria are complex, cumbersome, easy to pollute, and expensive

Method used

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  • A kind of human mycoplasma pneumoniae culture medium and its diagnostic kit
  • A kind of human mycoplasma pneumoniae culture medium and its diagnostic kit
  • A kind of human mycoplasma pneumoniae culture medium and its diagnostic kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Preparation of Human Mycoplasma Pneumonia Liquid Medium:

[0042]

[0043] Preparation and packaging:

[0044] 1. According to the amount of medium to be prepared, weigh the reagents and mix them thoroughly (such as solid medium plus 10-15g agar).

[0045] Among them, the preparation method of poultry egg yolk extract is as follows: (1) Take fresh selenium-enriched eggs and soak them in 70v / v% ethanol solution for 30 minutes; (2) Take the egg yolk aseptically on the ultra-clean workbench, and place the egg yolk In a sterile flask; (3) Add 90v / v% ethanol to a sterile flask containing egg yolk at a weight ratio of 1:2, stir and mix thoroughly, seal the mouth of the bottle and place it at 4℃~8℃, Store for 20-24h; (4) Adjust the pH of the solution after treatment in step (3) with 1N NaoH to 7.0~8.0, centrifuge at 6000RPM, take the supernatant, and filter the supernatant aseptically with a 0.45μm filter membrane. It is the poultry egg yolk extract, and the filtrate is aseptically...

Embodiment 2

[0054] Preparation of Human Mycoplasma Pneumonia Liquid Medium:

[0055]

[0056] The preparation method is the same as in Example 1.

Embodiment 3

[0058] Human Mycoplasma Pneumoniae Solid Medium—Preparation of Slide Type Culture Box:

[0059]

[0060]

[0061] Add to:

[0062]

[0063] Bacteriostatic agent: 1 million units of ampicillin, 50 mg of natamycin.

[0064] After mixing uniformly, use aseptic operation to add 4-5ml to the culture box, and cover after cooling.

[0065] Other preparation methods are the same as in Example 1.

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Abstract

The invention relates to a human mycoplasma pneumoniae culture medium and a diagnosis kit thereof. The human mycoplasma pneumoniae culture medium comprises a TSB culture medium and an additive, wherein the additive comprises bovine serum, DMEM (dulbecco's modified eagle medium), avian yolk extract, sodium pyruvate and glutamine. The DMEM accounts for 5-15 vol% of the human mycoplasma pneumoniae culture medium. On the basis of the total volume of the human mycoplasma pneumoniae culture medium, the avian yolk extract content is 10-100 ml / L, the sodium pyruvate content is 0.2-1.0 g / L, and the glutamine content is 0.3-1.0 g / L. Compared with the prior art, the culture medium provided by the invention performs practical innovation design on the basis of abundant practices, conforms to the demands, ensures the specificity and reliability of the clinical Mp infection diagnosis, is convenient to use, and has the advantages of high speed, high safety, environment friendliness and resource saving.

Description

Technical field [0001] The invention relates to a mycoplasma culture medium and a diagnosis kit thereof, in particular to a human mycoplasma pneumoniae culture medium and a diagnosis kit thereof. Background technique [0002] Mycoplasma, which has been discovered for nearly a hundred years, is a type of prokaryotic microorganism without cell wall, which lies between viruses and bacteria. At present, more than 150 kinds have been separated. Mainly related to human diseases are Mycoplasma pneumoniae (Mycoplasmapneumoniae, Mp), Ureaplasma urealyticum (Uu) and Mycoplasma hominis (Mh) and so on. Mp has long been proven to be an important pathogen of atypical pneumonia, which is difficult to diagnose, easily cause concurrent infections, and has a high mortality rate. After the SARS epidemic in 2003, when community respiratory infections were prevalent, it attracted more attention from the medical profession. [0003] Mp can enter the blood and multiply outside the respiratory system, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12Q1/04C12R1/35
CPCC12N1/20C12Q1/045
Inventor 文凤岐武济民孙亚洲肖家祁
Owner 众爱生河北生物科技有限公司
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