67results about How to "No obvious inhibitory effect" patented technology

The invention discloses a pyridines compound for treating liver diseases. The compound is a compound as shown in a formula I, pharmacologically acceptable salt, a hydrate, a solvate or a metabolite ofthe compound. The compound can be used for preparing a medicine for treating and / or preventing the liver diseases.

The invention relates to a preparation for treating treating keratomycosis, and particularly relates to a long-acting slow release preparation for treating keratomycosis as well as a preparation method and application thereof. The substrate of the long-acting slow release preparation is formed through electrostatic bonding of a graphene material and a drug-carried chitosan material, drugs are carried on the sheet structure of the graphene material, the structure is stable, and the loading rate is high. The preparation provided by the invention has excellent bacteriostatic activity on fungus and bacterium, and is excellent in cytocompatibility, toughness, and tensile strength. The preparation can be simply and conveniently applied onto cornea, and has no toxic effects on normal tissue while achieving excellent bacteriostatic activity. The long-acting slow release preparation is taken as a dosage form in ophthalmology, can be adhered onto a cornea of a patient for long-acting slow release, so as to achieve effective drug concentration, and the preparation has the advantages that the preparation is simple and convenient, the drug-carried material self is excellent in bacteriostatic activity and mechanical property, stimulation and toxic or side effects on orbital tissue can be avoided, and the use is convenient.

The invention provides a method for evaluating the unique nitrosamine inhibiting function of additive on tobacco. The method comprises the following steps: a, blank system reaction, in which, nicotine and nitrite are mixed in buffer solution according to a molar ratio of 1:1-5, and react under a certain condition; b, inhibiting system reaction, in which, the nicotine and the nitrite are mixed according to the same proportion of the step a, after additive in certain amount is added to the mixture, and the mixture perform reaction according to the same buffer system, the same temperature and time of the step a; and c, respective extraction and separation, measuring TSNAs contents in the blank system and the inhibiting system respectively by a GC-TEA method to compare differences of the two systems and evaluate the inhibiting effects of the additive. The method has the advantages that a quick, accurate and scientific evaluation method is established, and the inhibiting effect of various additives on TSNAs generation can be objectively evaluated through specific measurement data, so that initial screen can be carried out on different additives which are planned to apply to tobacco.

The invention relates to ainsliaea fragrans champ caffeoylquinic acid extracts and preparation and application thereof. The ainsliaea fragrans champ caffeoylquinic acid extracts are prepared by the following steps of: performing refluxing extraction of the ainsliaea fragrans champ by using ethanol, filtering, concentrating and removing ethanol, adjusting the pH value to 1 to 3 by adding hydrochloric acid, applying macroporous adsorbent resin, water-washing, adding ethanol for gradient elution, and collecting eluent, of which the ethanol concentration is 50 to 70 percent; performing condensation and ethanol removal, adjusting the pH value to 1 to 3 by adding hydrochloric acid, using macroporous adsorbent resin, adding water and ethanol for gradient elution respectively, and collecting the eluent, of which the ethanol concentration is 40 to 70 percent; mixing the eluent, and vacuum drying the eluent to obtain the extracts. The UV content of the ainsliaea fragrans champ caffeoylquinic acid extracts is over 70 percent, the HPLC content is over 50 percent, and the content of 3-caffeoylquinic acid and the content of 3,5-dicaffeoylquinic acid are both over 10 percent. The ainsliaea fragrans champ caffeoylquinic acid extracts have the effects of resisting inflammations, bacteria, medicament-resistant strains, well improves pathologically changed tissues of rat suffering from cervicitis, obviously reduces the uterus index and the number of leukocytes and can be independently prepared into the medicaments for treating cervicitis or combined with other medicaments to prepare the medicaments for treating cervicitis.

The invention belongs to the pharmaceutical chemistry field, and relates to a 2,3-dihydrobenzazapine*compound with a structure shown in a formula I, the 2,3-dihydrobenzazapine*compound is capable of inhibiting glycogen synthase kinase-3beta(GSK-3beta), and is a small-molecule inhibitor of GSK-3beta, a non ATP competition effect mode is taken for inhibiting the activity of GSK-3beta, no obvious inhibition effect is provided on the tested various protein kinase, and the 2,3-dihydrobenzazapine*compound has high kinases selectivity, and can be used for preparing medicines for preventing or treating the diseases due to GSK-3beta abnormity. The invention further comprises an application of a pharmaceutically acceptable salt or a pharmaceutical composition of the compound for preventing or treating the relative diseases to GSK-3beta, the relative diseases to GSK-3beta are diabetes, Alzheimer's disease, stomach cancer, colorectal cancer, pancreas cancer, liver cancer or mixed acute leukemia, but the relative diseases are not limited by the above diseases.

The invention discloses a biological composite oxygen producer, which is prepared from the following raw materials by weight: 1.5-3 parts of a chemical oxygen producer, 1 part of a biological agent, 0.1-0.2 parts of sodium citrate and 0.6-1 part of an adhesive. The chemical oxygen producer is prepared by mixing anhydrous sodium carbonate and hydrogen peroxide in the mass ratio of 5: 3, and adding sodium chloride in the mixing process. The biological agent is a mixed powder of one or two of Bacillus subtilis N9-1-35 and Bacillus subtilis B7348 at an arbitrary ratio. The invention also discloses a preparation method of the biological composite oxygen producer. The biological composite oxygen producer provided by the invention effectively combines the biological agent with the chemical oxygen producer, so that the biological agent and oxygen producer can reach the bottom of the pond at the same time; the oxygen producer releases oxygen to increase the dissolved oxygen at the bottom, and the biological agent can decompose harmful materials at the bottom, degrade ammonia content and play a role in regulating water quality and balancing the microecology of the water, so as to fundamentally ease the problem of pond hypoxia.

The indirubin dripping pill containing indirubin 1-12 mg and proper amount of dripping pill medium in each pill. The dripping pill medium is one or two of polyglycol-6000, polyglycol-4000, polyoxyethylene monostearate, sodium stearate, glycerin, gelatin, poloxamer, stearic acid, glycerin monostearate and insect wax. The preparation process of the indirubin dripping pill includes melting and mixing indirubin and dripping pill medium, dropping at 60-120 deg.c into condensating agent, and eliminating condensating agent. The condensating agent is one or two of dimethyl silicon oil, liquid paraffin, tea seed oil and rape seed oil. The indirubin dripping pill is dark red. The indirubin dripping pill is used in treating chronic granulocyte leukaemia, and has the features of less side effect, low dosage, high medicinal effect, no obvious inhibition on marrow of patient, etc.

The invention discloses a microbial flora for treating high-salt industrial wastewater and aims to screen and compound functional florae and verify the application effects on the basis of the characteristics that high-salt wastewater is high in salt content, high in chromaticity, high in COD, low in BOD and poor in biochemical property and contains complicated contaminants. Through isolation, screening and acclimatization, an active flora is obtained, the active flora has the capability of tolerating 18% high-salt wastewater and can be applied to biochemical treatment on high-salt pickling wastewater; according to the components and the proportion of the flora, relevant strains are purchased from the China General Microbiological Culture Collection Center and used for simulating the flora components of the acclimatized microbial flora, that is, enterobacter hormaechei, bacillusmegatherium, chromohalobacterjaponicas, halomonasxianhensis, chryseobacteriumindoltheticum and clostridiumsporogenes are compounded in a quantity ratio of 2:2:1:1:1:1, a relatively remarkable degradation effect is achieved when the compounded microbial flora is used for treating wastewater, COD in high-salt wastewater can be degraded to 1935.62mg / L from 29465mg / L, the COD degradation rate is 93.43%, and an effective technical support is provided for biological treatment on high-salt industrial wastewater which is hard to treat.

The invention belongs to the technical field of molecular biology and particularly relates to a portunus trituberculatus PtCrustin-2 gene, and coded protein and application thereof. The PtCrustin-2 gene DNA is amplified from portunus trituberculatus by utilizing a cDNA library and an RACE technology and the gene exerts important effect in the aspect of immunologic defence of the portunus trituberculatus. The recombinant PtCrustin-2 protein has obvious bacteriostatic activity on Gram negative bacteria and Gram positive bacteria. The minimum inhibitory concentrations of the gene on vibrio alginolyticus, pseudomonas aeruginosa, staphylococcus aureus and micrococcus luteus are 24.75 MuM, 12.37 MuM, 49.51 MuM and 49.51 MuM respectively. The gene does not have obvious bacteriostatic effect on fungi pichia pastoris. Basis is laid for diseases prevention and treatment on the portunus trituberculatus, gene auxiliary breeding and development of feed additives.

The invention discloses an efficient dephosphorization agent for waste water treatment. The efficient dephosphorization agent is prepared from the following components in percentage by weight: 20 to 30 percent of aluminum sulfate, 20 to 30 percent of ferric sulfate, 10 to 20 percent of sodium hypochlorite and 30 to 40 percent of water. The efficient dephosphorization agent belongs to energy-saving, qualified, efficient and environment-friendly water treatment medicament; and the dephosphorization effect of the efficient dephosphorization agent is more than three times that of the conventional water treatment chemical, so the efficient dephosphorization agent can meet the marketing requirement.

The invention discloses a transparent water-absorbing gel for microbial detection and detection plate. A solid matter of the transparent water-absorbing gel consists of polyacrylic resin, sodium carboxymethylcellulose and sodium polyacrylate. The transparent water-absorbing gel disclosed by the invention has the characteristics of rapid water absorption and water retention, and ensures that a diluent can be rapidly absorbed by the gel, is uniform in distribution, and is not easy to flow on the gel surface in strands; and meanwhile, the gel has the advantages of good coagulability and high transparency, does not have obvious inhibition effects on microorganism growth, is beneficial to accurate reading, and ensures that more accurate detection results can be acquired. By adopting the transparent water-absorbing gel, a series of products such as Listeria monocytogenes detection plates, staphylococcus aureus detection plates, salmonella detection plates and escherichia coli O157 detection plates can be successfully developed, and pathogenic microorganisms in foods and environments can be rapidly detected.

The invention discloses application of an efficient dephosphorization agent in wastewater treatment. The efficient dephosphorization agent is prepared from the following components by weight percent: 20-30% of aluminum sulfate, 20-30% of ferric sulfate, 10-20% of sodium hypochlorite and 30-40% of water. According to the invention, municipal sewage and industrial sewage are treated by using the efficient dephosphorization agent, and the phosphorus-removing effect is above three times more than that of the traditional water treatment chemicals; the efficient dephosphorization agent has obvious auxiliary effects in the aspects of sterilization, deodorization and water removal from foam, float mud and filthy mud; a good technical support is provided for filthy mud reduction in production operation; and good environment effects are obtained.

The invention belongs to the technical field of molecular biology, and particularly discloses a PtCrustin-1 gene of Portunus trituberculatus, encoding protein of the PtCrustin-1 gene and application of the PtCrustin-1 gene. Complementary deoxyribonucleic acid (cDNA) of the PtCrustin-1 gene is amplified from the Portunus trituberculatus by using a cDNA library and rapid-amplification of cDNA ends (RACE); and the gene has an important effect in the aspect of immune defence of the Portunus trituberculatus. Recombinant PtCrustin-1 protein has a remarkable effect of inhibiting the growth of Gram-negative bacteria, namely Pseudomonas aeruginosa and Gram-positive bacteria, namely Staphylococcus aureus and Micrococcus luteus, and the minimum inhibitory concentrations are 1.50mu M, 24.03mu M, 24.03mu M and 48.06mu M respectively; and the recombinant PtCrustin-1 protein does not have a remarkable effect of inhibiting the Gram-negative bacteria, namely Vibrio alginolyticus and a fungus, namely Pichia pastoris. The invention lays a foundation for the disease control, gene-assisted breeding, and development of feed additives of the Portunus trituberculatus.

The invention belongs to the technical field of water environment bacteriostatic agents, and discloses a composite plant extract for inhibiting aeromonas schubertii in a water environment. The composite plant extract belongs to an environment-friendly substance, has a large safe concentration range on fish bodies, does not cause chemical drug residues in the fish bodies, does not easily cause drug resistance of aeromonas schubertii, and does not damage algae in water. The action spectrum of the compound extract only has a special effect on aeromonas schubertii, and the compound extract can be used for inhibiting abnormal reproduction of aeromonas schubertii in a culture water environment, so that the aggressive force of aeromonas schubertii in the water environment on fish bodies is reduced. From the aspect of formula compatibility, the four components of the compound plant extract have a remarkable compatibility synergistic effect, not only have a good inhibiting effect on aeromonas schubertii in a culture water body, but also have remarkable anti-inflammatory and astringing effects on local red spots on fish body surfaces caused by aeromonas schubertii infection.

The invention relates to ainsliaea fragrans champ caffeoylquinic acid extracts and preparation and application thereof. The ainsliaea fragrans champ caffeoylquinic acid extracts are prepared by the following steps of: performing refluxing extraction of the ainsliaea fragrans champ by using ethanol, filtering, concentrating and removing ethanol, adjusting the pH value to 1 to 3 by adding hydrochloric acid, applying macroporous adsorbent resin, water-washing, adding ethanol for gradient elution, and collecting eluent, of which the ethanol concentration is 50 to 70 percent; performing condensation and ethanol removal, adjusting the pH value to 1 to 3 by adding hydrochloric acid, using macroporous adsorbent resin, adding water and ethanol for gradient elution respectively, and collecting the eluent, of which the ethanol concentration is 40 to 70 percent; mixing the eluent, and vacuum drying the eluent to obtain the extracts. The UV content of the ainsliaea fragrans champ caffeoylquinic acid extracts is over 70 percent, the HPLC content is over 50 percent, and the content of 3-caffeoylquinic acid and the content of 3,5-dicaffeoylquinic acid are both over 10 percent. The ainsliaea fragrans champ caffeoylquinic acid extracts have the effects of resisting inflammations, bacteria, medicament-resistant strains, well improves pathologically changed tissues of rat suffering from cervicitis, obviously reduces the uterus index and the number of leukocytes and can be independently prepared into the medicaments for treating cervicitis or combined with other medicaments to prepare the medicaments for treating cervicitis.

The invention belongs to the technical field of water environment bacteriostatic agents, and discloses a composite plant extract for water environment vibrio parahaemolyticus, which is mainly prepared from the following components in percentage by mass: 35%-55% of a gerbera piloselloides extract, 30%-42% of a roughhaired holly extract, 5%-12% of a gynura bicolor flower extract and 5-12% of a dandelion extract. Compared with traditional GMP disinfectants, the compound plant extract belongs to an environment-friendly substance, is large in the safety concentration range of shrimps, and has an obvious inhibiting effect on vibrio parahaemolyticus when being used for splashing chemical water and cannot be interfered by organic matters in water; when the use dosage is smaller than or equal to 5 mg / L, abnormal propagation of vibrio parahaemolyticus in upper-layer, middle-layer and bottom-layer culture of a culture water body can be effectively inhibited, and the safety to pelteobagrus fulvidraco, ictalurus punctatus and silurus meridionalis is good.

The invention relates to the technical field of photocatalysis, and particularly discloses application of a G-C3N4 catalyst in degradation of organic dyes in high-salinity wastewater. The applicationof the catalyst in degradation of organic dyes in high-salinity wastewater has the advantages of simple process, low cost and environmental protection performance, organic dyes in wastewater can be efficiently removed in a wide sodium chloride concentration range, and environmental pollution is reduced.

The invention belongs to the technical field of molecular biology, in particular relates to a Portunus trituberculatus anti-lipopolysaccharide factor PtALF-5 gene and encoding proteins and application thereof. In the invention, a PtALF-5 gene cDNA is amplified from a Portunus trituberculatus by utilizing a cDNA (Complementary Deoxyribonucleic Acid) library and an RACE (Rapid-amplification of cDNA Ends) technology and plays an important role in immune defense of the Portunus trituberculatus. A recombinant PtALF-5 protein has stronger antibacterial activity to Gram-negative Vibrio alginolyticus and P. Aeruginosa, and the minimal inhibitory concentrations are respectively 7.77 mu M and 31.08 mu M; but the recombinant PtALF-2 protein has no obvious inhibitory action to Gram-positive Staphylococcus aureus, Micrococcus luteus and fungus Pichia pastoris. The invention lays a foundation for disease prevention and control, gene-assisted selection and development of feed additives of the Portunus trituberculatus.

The invention belongs to the technical field of molecular biology, in particular relates to a Portunus trituberculatus anti-lipopolysaccharide factor PtALF-2 gene and encoding proteins and application thereof. In the invention, a PtALF-2 gene cDNA is amplified from a Portunus trituberculatus by utilizing a cDNA (Complementary Deoxyribonucleic Acid) library and an RACE (Rapid-amplification of cDNA Ends) technology and plays an important role in immune defense of the Portunus trituberculatus. A recombinant PtALF-2 protein has obvious antibacterial activity to Gram-negative Vibrio alginolyticus, P. Aeruginosa, Edwardsiella tarda, Gram-positive Staphylococcus aureus and Micrococcus luteus, and the minimal inhibitory concentrations are respectively 1.02 mu M, 2.04 mu M, 4.07 mu M, 16.30 mu M and 65.19 mu M; but the recombinant PtALF-2 protein has no obvious inhibitory action to fungus Pichia pastoris. The invention lays a foundation for disease prevention and control, gene-assisted selection and development of feed additives of the Portunus trituberculatus.

The invention relates to 68Ga-NODAGA-cyclic polypeptide FG01 of a targeted EphA2 receptor as well as a preparation method and application of the 68Ga-NODAGA-cyclic polypeptide FG01. The 68Ga-NODAGA-cyclic polypeptide FG01 forms an amido bond through an amino group of lysine in an amino acid sequence and a carboxyl group of serine, then an amino group at an amino terminal of the cyclic polypeptide FG01 and a coupling agent NODAGA-NHS are subjected to a condensation reaction, a chelating agent NODAGA in the cyclic polypeptide conjugate NODAGA-FG01 is chelated with 68Ga, and the 68Ga-labeled cyclic polypeptide conjugate is constructed. The invention verifies that the annular polypeptide FG01 has no obvious inhibition effect on tumor cells, indicates that the annular polypeptide FG01 has no cytotoxicity, and finds that the 68Ga-NODAGA-annular polypeptide FG01 of a targeted EphA2 receptor can be used for in-vivo imaging of EphA2 high-expression tumors, realizes molecular imaging of the EphA2 high-expression tumors such as non-small cell lung cancer by utilizing PET (Polyethylene Terephthalate), and is widely used as a tumor molecular imaging agent.

The transfused gingko leaf liquid is prepared with the materials including gingko leaf, absolute alcohol, povidone K30, sodium chloride, antioxidant and injection water and no sorbierite. During itspreparation, gingko leaf extract is first extracted with gingko leaf and through hot soaking, filtering, column refining and other steps and then mixed with other materials. The transfused gingko leaf liquid can improve body's blood circulation, dilute coronary artery and cerebral artery and may be used in treating coronary heart disease, angina pectoris, cerebral thrombus, cerebral malfunction,ischemic cerebral apoplexy, etc. and improving the perception function of senile dementia and vascular dementia patients.

The invention belongs to the field of diagnosis and treatment of tumors, and relates to a hypoxia inducible factor (HIF)-2 alpha small-molecule inhibitor and use thereof in treatment of breast cancer,ovarian cancer and other tumors. The small-molecule inhibitor is a compound shown in a formula (I) or stereoisomers, pharmaceutically acceptable salts, solvates or prodrugs of the compound shown in the formula (I); the proliferation of tumor cells is inhibited mainly by inhibiting HIF-2 alpha protein; furthermore, the HIF-2 alpha small-molecule inhibitor can inhibit the growth of CSCs with high expression of HIF-2 alpha, and can be used as a new anti-tumor drug to completely remove tumors and stem cells, thus having a broad application prospect.

The invention belongs to the technical field of molecular biology, and in particular relates to a Portunus trituberculatus anti-lipopolysaccharide factor PtALF-1 gene, a protein encoded by the gene and an application of the protein. A cDNA (complementary Deoxyribose Nucleic Acid) library and a RACE (Rapid Amplification of cDNA Ends) technology are utilized for amplification to obtain PtALF-1 gene cDNA from the Portunus trituberculatus. The gene performs an important action on the immunologic defense aspect of the Portunus trituberculatus. The recombinant PtALF-1 protein has obvious Gram-negative bacterium and gram-positive bacterium bacteriostatic activity, has the minimum inhibitory concentration on Vibrio alginolyticus, pseudomonas aeruginosa, Staphylococcus aureus and the Micrococcus luteus of respectively 2.33mu M, 18.64mu M, 18.64mu M and 18.64mu M, and does not have obvious inhibition action on fungus Pichia pastoris. The invention lays the foundation on prevention and control of diseases and insect pests, gene-assisted breeding and development of feed additive of Portunus trituberculatus.

The invention discloses a new use of paeonol and metabolites thereof, particularly relates to a new use of the paeonol metabolites including 2-hydroxy-4-methoxyacetophenone-5-O-sulfate(M2) and 2,5-dihyfroxy-4-methoxyacetophenone(M5) in preparation of anti-tumor drugs, especially in the treatment of liver cancer and malignant melanoma, and further specifically relates to a new use of the paeonol and metabolites M1-M6 thereof in preparation of drugs for treating diabetes. The metabolites M2 and M5 can inhibit the growth of human hepatoma cells and melanoma cells, and especially have an obvious inhibition effect on the growth of A375 cells and Hep-G2 cells; 10 micrometers of the paeonol and the metabolites M1-M6 thereof are not toxic to 3T3-L1 cells; at the dose, the paeonol and the metabolites M1-M6 thereof can significantly increase glucose uptake, and the effect is similar to that of positive drugs; the metabolites M1-M6 of the paeonol can be applied to prepare the drugs for treating the diabetes.

The invention discloses a traditional Chinese medicine composition for relieving inflammatory reaction of osteoarthritis and a preparation method thereof. The traditional Chinese medicine compositionis prepared from the following components in parts by mass: 100-140 parts of common clubmoss herb, 100-140 parts of trberculate speranskia herb, 100-140 parts of doubleteeth pubescent angelica roots,100-140 parts of Chinese clematis roots, 100-140 parts of Chinese angelica, 100-140 parts of borneol and 100-140 parts of medicinal cyathula roots. The traditional Chinese medicine composition does not contain toxic traditional Chinese medicine components indicated in a pharmacopoeia. Cytotoxicity evaluation is carried out on the medicine composition in liver and kidney cells, and according to thecytotoxicity evaluation, the medicine composition has no obvious inhibition effect on activity of liver and kidney cells and can be taken for a long time.

The invention belongs to the technical field of molecular biology, and particularly relates to a portunus trituberculatus antilipopolysaccharide factor PtALF-4 gene and coding protein and applications thereof. The gene is amplified to the PtALF-4 gene cDNA from the portunus trituberculatus by utilizing a cDNA library and an RACE (rapid-amplification of cDNA ends) technology and plays an important role in the aspect of the immunologic defense of the portunus trituberculatus. The recombined PtALF-4 protein has stronger bacteriostatic activity for Gram-negative bacterium vibrio alginolyticus, pseudomonas aeruginosa, wdwardsiella tarda and fungal pichia pastoris, and the minimum inhibitory concentrations are 3.32muM, 53.14muM, 13.28muM and 106.28muM, however, the recombined PtALF-4 protein has no obvious inhibitory action on the Gram-positive bacterium staphylococcus aureus and micrococcus luteus. The invention lays the foundation for the disease control of the portunus trituberculatus, the auxiliary breeding of the gene and the development of the feed additive.

The invention provides a physical and chemical synergistic anti-fouling type seawater uranium extraction adsorbent and a preparation method thereof. The method includes the steps that firstly, a two-dimensional carbon-based material is dispersed into methanol, a chemical anti-fouling agent and central metal ions are added, and ultrasonic treatment is carried out; then, a 2-methylimidazole methanolsolution is added for a reaction; then, the reaction product is washed with absolute ethanol and methanol respectively and subjected to centrifugal separation; finally, drying is carried out. The inhibition ratio of the adsorbent on phaeodactylum tricornutum can reach 90% or above; due to the synergistic effect of the two-dimensional carbon-based material and the doped metal ions on an imidazateframework material, the saturated adsorption capacity of the adsorbent can be increased by 120-150% compared with pure MOF and carbon-based materials, the optimal pH condition for overall adsorption of the adsorbent is improved at the same time and is closer to that of the seawater environment, and thus the adsorption efficiency can be improved.

The invention discloses a microbial flora for treating high-salt industrial wastewater and aims to screen and compound functional florae and verify the application effects on the basis of the characteristics that high-salt wastewater is high in salt content, high in chromaticity, high in COD, low in BOD and poor in biochemical property and contains complicated contaminants. Through isolation, screening and acclimatization, an active flora is obtained, the active flora has the capability of tolerating 18% high-salt wastewater and can be applied to biochemical treatment on high-salt pickling wastewater; according to the components and the proportion of the flora, relevant strains are purchased from the China General Microbiological Culture Collection Center and used for simulating the flora components of the acclimatized microbial flora, that is, enterobacter hormaechei, bacillusmegatherium, chromohalobacterjaponicas, halomonasxianhensis, chryseobacteriumindoltheticum and clostridiumsporogenes are compounded in a quantity ratio of 2:2:1:1:1:1, a relatively remarkable degradation effect is achieved when the compounded microbial flora is used for treating wastewater, COD in high-salt wastewater can be degraded to 1935.62mg / L from 29465mg / L, the COD degradation rate is 93.43%, and an effective technical support is provided for biological treatment on high-salt industrial wastewater which is hard to treat.

The invention belongs to the technical field of medicines, and relates to an HIF-2 alpha small-molecule inhibitor as well as a preparation method and an application thereof. The HIF-2alpha small-molecule inhibitor is a compound as shown in a structural general formula I and a stereoisomer, a pharmaceutically acceptable salt, a hydrate, a solvate or a prodrug thereof. According to the invention, molecular simulation drug design software MOE is used for screening and molecular docking of pharmacophores, according to a final docking dominant structure and an isosteric principle, a series of compounds are designed and synthesized, and an in-vitro anti-tumor activity screening test shows that the compounds have anti-tumor activity. The HIF-2 alpha small-molecule inhibitor provided by the invention has a relatively good application prospect in the aspect of preventing or treating cancers, particularly glioma.

The invention discloses an application of 1.6-hexanediol or its derivative in preparation of an antitumor drug. The 1.6-hexanediol or its derivative can destroy weak interaction of protein, inhibits aliquid-phase separating process, and can effectively inhibit the generation and development of tumor. The 1.6-hexanediol can relatively and specifically inhibit the cell proliferation of pancreas cancer, lung cancer and melanoma under low concentration condition, and has good application prospect on preparation of the antitumor drugs for treating pancreas cancer, lung cancer and melanoma.