Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Portunus trituberculatus anti-lipopolysaccharide factor PtALF-1 gene, protein encoded by gene and application of protein

A technology for resisting lipopolysaccharide factor and swimming crab, which is applied in the field of molecular biology and can solve the problem of less anti-lipopolysaccharide factor in swimming crab

Inactive Publication Date: 2012-01-25
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] So far, there are few reports on the anti-lipopolysaccharide factors of Portunus trituberculatus

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Portunus trituberculatus anti-lipopolysaccharide factor PtALF-1 gene, protein encoded by gene and application of protein
  • Portunus trituberculatus anti-lipopolysaccharide factor PtALF-1 gene, protein encoded by gene and application of protein
  • Portunus trituberculatus anti-lipopolysaccharide factor PtALF-1 gene, protein encoded by gene and application of protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The anti-lipopolysaccharide factor PtALF-1 gene of Portunus trituberculatus is the base sequence shown in SEQ ID No.1.

[0026] The sequence listing SEQ ID No.1 is:

[0027] ggggagtctc ggctcgaaca acagtgcgag taagctctac accccagcaca

[0028] tcctgagcca gccatcaga atg gca cgc gtg tcg ctt ctt ctc

[0029] atc gtg cta tcc att gct ctt gtt gcc ccc agt caa ggc ttc

[0030] ctg aaa gac cta ctc ttc ggt gaa gcg aaa aca gct ttg ctt

[0031] gag gat ggg aca aca gag atc ctt gac cat gtt tgc aac ttc

[0032] cga gtg atg ccc cgt tta aga agt tgg gag ctg tac ttc agg

[0033] gga gat gtg tgg tgc ccc ggc tgg aca gtc atc aag gga gaa

[0034] tcc ctg act cgc agg agg act agg gtg gtg aac aag gcc gtc

[0035] gca gac ttc gcc cag aaa gct ctc gct cag ggc ctc atc acg

[0036] cag gag gac gcc caa ccc ttg cta gag tag cttggaccta

[0037] caggcgtgtg tgccaaagag gtgcaaagac ggaatgatct cttcactatt

[0038] catactcccg tgaactccta tatcacgccg tcacccaatc tcgctctgcc

[0039] aacactattt tccttattat tctgttta...

Embodiment 2

[0073] The base sequence of Portunus trituberculatus anti-lipopolysaccharide factor PtALF-1 is described in SEQ ID No. 1 in the sequence listing, and the amino acid sequence is described in SEQ ID No. 2 in the sequence listing.

[0074] The sequence listing SEQ ID No.2 is:

[0075] Met Ala Arg Val Ser Leu Leu Leu Ile Val Leu Ser Ile Ala Leu Val

[0076] Ala Pro Ser Gln Gly Phe Leu Lys Asp Leu Leu Phe Gly Glu Ala Lys

[0077] Thr Ala Leu Leu Glu Asp Gly Thr Thr Glu Ile Leu Asp His Val Cys

[0078] Asn Phe Arg Val Met Pro Arg Leu Arg Ser Trp Glu Leu Tyr Phe Arg

[0079] Gly Asp Val Trp Cys Pro Gly Trp Thr Val Ile Lys Gly Glu Ser Leu

[0080] Thr Arg Ser Arg Thr Arg Val Val Asn Lys Ala Val Ala Asp Phe Ala

[0081] Gln Lys Ala Leu Ala Gln Gly Leu Ile Thr Gln Glu Asp Ala Gln Pro

[0082] Leu Leu Glu

[0083] It has a complete coded protein containing 115 amino acids, of which 1-21 amino acids of the coding sequence are signal peptides, and the mature peptide contains 94 amino...

Embodiment 3

[0087] In vitro antibacterial test of the anti-lipopolysaccharide factor PtALF-1 recombinant protein of Portunus trituberculatus:

[0088] 1. Culture and preparation of microorganisms: TSB medium for Vibrio alginolyticus at 28°C, TSB medium for Pseudomonas aeruginosa at 37°C, LB medium for Staphylococcus aureus at 37°C, and LB medium for Micrococcus luteus 37°C, Pichia pastoris with YPD medium at 28°C, and the above-mentioned strains were cultured on a shaker at 220rpm / min so that the bacterial concentration reached the logarithmic growth phase, and the bacteria were diluted with 50mM Tris-HCl (pH=8.0) buffer, respectively. The number of colonies per milliliter of bacterial liquid is about 1×10 3 .

[0089] 2. Determination of antibacterial activity of recombinant protein PtALF-1: Utilize the recombinant protein PtALF-1 obtained in the above-mentioned examples to use Tris-HCl (50mM, pH=8.0) gradient dilution (1, 1 / 2, 1 / 4, 1 / 8, 1 / 16, 1 / 32, 1 / 64), 50 μl was added to a sterile ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of molecular biology, and in particular relates to a Portunus trituberculatus anti-lipopolysaccharide factor PtALF-1 gene, a protein encoded by the gene and an application of the protein. A cDNA (complementary Deoxyribose Nucleic Acid) library and a RACE (Rapid Amplification of cDNA Ends) technology are utilized for amplification to obtain PtALF-1 gene cDNA from the Portunus trituberculatus. The gene performs an important action on the immunologic defense aspect of the Portunus trituberculatus. The recombinant PtALF-1 protein has obvious Gram-negative bacterium and gram-positive bacterium bacteriostatic activity, has the minimum inhibitory concentration on Vibrio alginolyticus, pseudomonas aeruginosa, Staphylococcus aureus and the Micrococcus luteus of respectively 2.33mu M, 18.64mu M, 18.64mu M and 18.64mu M, and does not have obvious inhibition action on fungus Pichia pastoris. The invention lays the foundation on prevention and control of diseases and insect pests, gene-assisted breeding and development of feed additive of Portunus trituberculatus.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and specifically relates to a gene of Portunus trituberculatus anti-lipopolysaccharide factor PtALF-1, its encoded protein and its application. Background technique [0002] Portunus trituberculatus (Portunus trituberculatus) is an important marine economic crab. Due to its rich nutritional value and rapid growth, it has become an important marine aquaculture species in my country. However, with the continuous expansion of the breeding scale and the continuous improvement of the degree of intensification, various diseases caused by bacteria, fungi, etc. frequently broke out in the Portunus trituberculatus breeding population, causing huge economic losses and seriously restricting the development of Portunus trituberculatus aquaculture industry. Healthy and sustainable development. [0003] Due to the lack of in-depth understanding of the immune system of Portunus trituberculatus, tradi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A23K1/16A23L3/3544C12N15/12A61P31/04A61P37/04A61K38/17C07K14/435A23K20/153
Inventor 崔朝霞刘媛
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com