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Composite plant extract for inhibiting aeromonas schubertii in water environment as well as preparation method and application of composite plant extract

A technology of plant extracts and Aeromonas, which is applied in the field of compound plant extracts and its preparation for inhibiting Aeromonas schubert in the water environment, which can solve the problem of rising pathogenic drug resistance, insufficient dosage, and potential safety hazards, etc. problem, to achieve the effect of inhibiting abnormal reproduction, good inhibitory effect, and reducing attack power

Active Publication Date: 2021-05-28
清远海贝生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] According to the principle of infectious disease prevention and control, that is, control the source of infection, cut off the route of transmission, and protect susceptible hosts, in current production practice, it is common to prevent and treat Aeromonas schubert by taking sensitive antibiotics orally and disinfecting externally. The antibiotics used in the above scheme will not only cause drug residues and affect food safety, but also easily lead to the rise of pathogenic drug resistance, which brings high difficulty to production and prevention of the disease; spilled disinfectants are mainly used to cut off diseased fish. As the transmission route of the secondary infection source, the dosage is often insufficient due to cost factors and safety upper limit concentration, resulting in the phenomenon that the effect of adjuvant therapy is greatly weakened
In the pre-epidemic or epidemic period of the disease, it is the best way to effectively prevent the outbreak of Aeromonas Schubert's disease by inhibiting the rapid reproduction of Aeromonas Schubertii in water and enhancing the disease resistance of fish. To avoid the phenomenon of rapid spread of the disease after the occurrence of the disease and cause the loss of dead fish, most of the antibacterial agents in the prior art are obtained by compounding chemical substances, which have great potential safety hazards and environmental hazards

Method used

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  • Composite plant extract for inhibiting aeromonas schubertii in water environment as well as preparation method and application of composite plant extract
  • Composite plant extract for inhibiting aeromonas schubertii in water environment as well as preparation method and application of composite plant extract
  • Composite plant extract for inhibiting aeromonas schubertii in water environment as well as preparation method and application of composite plant extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] A compound plant extract for inhibiting Aeromonas schuberteus in the water environment, by weight percentage, weigh 38 parts of the litchi peel extract, 42 parts of Jinbuhuan fresh leaf extract, fern root 19 parts of extract and 1 part of iodine-free seawater coarse salt, mix the above-mentioned components at 28°C, stir well until the iodine-free sea salt is completely dissolved, and the obtained mixed solution is the compound plant extract thing.

[0071] Wherein, the above-mentioned lychee peel extract is obtained through fermentation:

[0072](1) Litchi peel pretreatment: mix litchi peel, water, and cellulase at a ratio of 10:50: (1-1.5), place it at 30-33°C, stir once every 1-2 hours, and keep stirring 2-3 days, take out the above mixture, and dry it at 30-35°C until the water content is 10-12%;

[0073] (2) The weight-to-volume ratio of pretreated litchi peel: water: lactic acid bacteria liquid medium is 1:8:3, and the amount of lactic acid bacteria added is 4×10...

experiment example 1

[0076] Experimental Example 1 Composite Plant Extract of the present invention compares the inhibitory effect of common aquatic pathogenic bacteria

[0077] 1. Test material

[0078] 1.1 Test strains

[0079] Aeromonas schubert, Aeromonas hydrophila, Edwardsiella tarda, Nocardia: all provided by our research group.

[0080] Povidone-iodine: a 10% povidone-iodine solution.

[0081] 1.2 Test consumables

[0082] TSB medium, Petri dishes, test tubes, distilled water, etc.

[0083] 1.3 Compound plant extracts

[0084] The compound plant extract of the present invention used in this experiment, its preparation method is the same as embodiment 1.

[0085] 2. Test method

[0086] 2.1 Bacterial suspension preparation

[0087] Inoculate Aeromonas schubert, Aeromonas hydrophila, Edwardsiella tarda, and Nocardia in TSB nutrient broth, culture at 30°C for 16-20 hours, collect the bacteria by centrifugation, and use McFarland turbidimetry The method of turbidimetrically estimating ...

experiment example 2

[0105] Experimental example 2 The inhibitory effect of compound plant extract prescription compatibility of the present invention on Aeromonas schubert

[0106] 1. Test material

[0107] 1.1 Preparation of compound plant extracts

[0108] The composite plant extract of the present invention used in this experiment is named as Y, and its preparation method is the same as in Example 1.

[0109] 1.2 Preparation of compound plant extracts with missing prescriptions

[0110] Based on the compound plant extract formula prepared in Example 1 of the present invention, through the technical idea of ​​single-component deletion or double-component simultaneous deletion or three-component simultaneous deletion and the missing components are replaced by PBS in equal amounts to prepare Compound plant extract with single deletion of litchi peel extract, compound plant extract with single deletion of Jinbuhua fresh leaf extract, compound plant extract with single deletion of fern root extra...

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Abstract

The invention belongs to the technical field of water environment bacteriostatic agents, and discloses a composite plant extract for inhibiting aeromonas schubertii in a water environment. The composite plant extract belongs to an environment-friendly substance, has a large safe concentration range on fish bodies, does not cause chemical drug residues in the fish bodies, does not easily cause drug resistance of aeromonas schubertii, and does not damage algae in water. The action spectrum of the compound extract only has a special effect on aeromonas schubertii, and the compound extract can be used for inhibiting abnormal reproduction of aeromonas schubertii in a culture water environment, so that the aggressive force of aeromonas schubertii in the water environment on fish bodies is reduced. From the aspect of formula compatibility, the four components of the compound plant extract have a remarkable compatibility synergistic effect, not only have a good inhibiting effect on aeromonas schubertii in a culture water body, but also have remarkable anti-inflammatory and astringing effects on local red spots on fish body surfaces caused by aeromonas schubertii infection.

Description

technical field [0001] The invention relates to the technical field of antibacterial agents for water environment, and more specifically, relates to a compound plant extract for inhibiting Aeromonas schubertius in water environment, its preparation method and application. Background technique [0002] Aeromonas schubert is a common opportunistic pathogen, which can cause septicemia and / or visceral knots in fishes of the family Channaidae, which tends to occur in raw fish of old fish shed period of 4-5 months and large body weight stage and the stage of small body weight specifications for 6-8 months after raw fish and new fish are settled in the pond. Once the raw fish is infected, the diseased fish in the same pond are often used as the source of infection, and they often have strong infectivity and high lethality. The highest average daily death rate per mu can exceed 100 fish. Days can quickly and completely control the disease. [0003] According to the principle of in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/04A01N65/08A01N59/08A01P1/00A61K36/77A61P17/00A61P29/00A61P31/04C02F1/50A61K33/14
CPCA01N59/08A01N65/00A01N65/04A01N65/08A61K33/14A61K36/12A61K36/59A61K36/77A61P17/00A61P29/00A61P31/04C02F1/50A61K2300/00
Inventor 黄锦炉
Owner 清远海贝生物技术有限公司
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