Composite plant extract for inhibiting aeromonas schubertii in water environment as well as preparation method and application of composite plant extract
A technology of plant extracts and Aeromonas, which is applied in the field of compound plant extracts and its preparation for inhibiting Aeromonas schubert in the water environment, which can solve the problem of rising pathogenic drug resistance, insufficient dosage, and potential safety hazards, etc. problem, to achieve the effect of inhibiting abnormal reproduction, good inhibitory effect, and reducing attack power
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Embodiment 1
[0070] A compound plant extract for inhibiting Aeromonas schuberteus in the water environment, by weight percentage, weigh 38 parts of the litchi peel extract, 42 parts of Jinbuhuan fresh leaf extract, fern root 19 parts of extract and 1 part of iodine-free seawater coarse salt, mix the above-mentioned components at 28°C, stir well until the iodine-free sea salt is completely dissolved, and the obtained mixed solution is the compound plant extract thing.
[0071] Wherein, the above-mentioned lychee peel extract is obtained through fermentation:
[0072](1) Litchi peel pretreatment: mix litchi peel, water, and cellulase at a ratio of 10:50: (1-1.5), place it at 30-33°C, stir once every 1-2 hours, and keep stirring 2-3 days, take out the above mixture, and dry it at 30-35°C until the water content is 10-12%;
[0073] (2) The weight-to-volume ratio of pretreated litchi peel: water: lactic acid bacteria liquid medium is 1:8:3, and the amount of lactic acid bacteria added is 4×10...
experiment example 1
[0076] Experimental Example 1 Composite Plant Extract of the present invention compares the inhibitory effect of common aquatic pathogenic bacteria
[0077] 1. Test material
[0078] 1.1 Test strains
[0079] Aeromonas schubert, Aeromonas hydrophila, Edwardsiella tarda, Nocardia: all provided by our research group.
[0080] Povidone-iodine: a 10% povidone-iodine solution.
[0081] 1.2 Test consumables
[0082] TSB medium, Petri dishes, test tubes, distilled water, etc.
[0083] 1.3 Compound plant extracts
[0084] The compound plant extract of the present invention used in this experiment, its preparation method is the same as embodiment 1.
[0085] 2. Test method
[0086] 2.1 Bacterial suspension preparation
[0087] Inoculate Aeromonas schubert, Aeromonas hydrophila, Edwardsiella tarda, and Nocardia in TSB nutrient broth, culture at 30°C for 16-20 hours, collect the bacteria by centrifugation, and use McFarland turbidimetry The method of turbidimetrically estimating ...
experiment example 2
[0105] Experimental example 2 The inhibitory effect of compound plant extract prescription compatibility of the present invention on Aeromonas schubert
[0106] 1. Test material
[0107] 1.1 Preparation of compound plant extracts
[0108] The composite plant extract of the present invention used in this experiment is named as Y, and its preparation method is the same as in Example 1.
[0109] 1.2 Preparation of compound plant extracts with missing prescriptions
[0110] Based on the compound plant extract formula prepared in Example 1 of the present invention, through the technical idea of single-component deletion or double-component simultaneous deletion or three-component simultaneous deletion and the missing components are replaced by PBS in equal amounts to prepare Compound plant extract with single deletion of litchi peel extract, compound plant extract with single deletion of Jinbuhua fresh leaf extract, compound plant extract with single deletion of fern root extra...
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