Composite plant extract for inhibiting vibrio parahaemolyticus in water environment as well as preparation method and application of composite plant extract
A technology of plant extracts and vibrio hemolyticus, applied in the direction of botany equipment and methods, applications, plant growth regulators, etc., can solve the problems of water algae phase destruction, increased drug resistance of vibrio parahaemolyticus, and increased costs
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Embodiment 1
[0075] A compound plant extract for inhibiting Vibrio parahaemolyticus in the water environment. In terms of weight percentage, we weighed 45% of the extract of Dragon Ball Grass, 38% of the extract of Gangmei, 9% of the extract of Vibrio parahaemolyticus, and the extract of Dandelion 8%. Under the environment of 28°C, the above-mentioned components are mixed, and the conventional ultrasonic emulsification is uniform, and the obtained mixed solution is the compound plant extract.
[0076] Among them, the above-mentioned extract of Dragon Ball Grass is obtained by the following method: cut short Dragon Ball Grass, bake at 47°C for 8 hours, pass through a 20-mesh sieve to obtain a crushed product, and take 100 parts of Dragon Ball Grass pulverization, mix well with 1000 parts of water, then add 12000IU cellulase and papain complex, in 38°C environment, airtight, interval stirring, store for 68-72h, unpack, add 500 parts of water, add spores in liquid form 15 copies of culture m...
experiment example 1
[0080] Experimental Example 1 Composite Plant Extract of the present invention compares the inhibitory effect of common aquatic pathogenic bacteria
[0081] 1. Test material
[0082] 1.1 Test strains
[0083] Vibrio parahaemolyticus, Vibrio alginolyticus, Vibrio harveyi, Aeromonas hydrophila: all are preserved and provided by our research group.
[0084] Potassium persulfate: Potassium persulfate with a content of 50%.
[0085] 1.2 Test consumables
[0086] TSB medium, Petri dishes, test tubes, distilled water, etc.
[0087] 1.3 Compound plant extracts
[0088] The compound plant extract of the present invention used in this experiment, its preparation method is the same as embodiment 1.
[0089] 2. Test method
[0090] 2.1 Bacterial suspension preparation
[0091] Inoculate Vibrio parahaemolyticus, Vibrio alginolyticus and Vibrio harveii respectively into TSB nutrient broth containing 0.5% sodium chloride, culture at 28°C for 16-20h, collect the bacteria by centrifugat...
experiment example 2
[0110] Experimental Example 2 Inhibition of Composite Plant Extract Composition of the present invention on Vibrio parahaemolyticus
[0111] 1. Test material
[0112] 1.1 Preparation of compound plant extracts
[0113] The composite plant extract of the present invention used in this experiment is named as Y, and its preparation method is the same as in Example 1.
[0114] 1.2 Preparation of compound plant extracts with missing prescriptions
[0115] Based on the compound plant extract formula prepared in Example 1 of the present invention, through the technical idea of one-component deletion or two-component simultaneous deletion or three-component simultaneous deletion, and the missing components are replaced by PBS in equal amounts, preparing a compound plant extract with a single deletion of Dragon Ball extract, a compound plant extract with a single deletion of Plum extract, a compound plant extract with a single deletion of C. Preparation of the compound plant extra...
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