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Composite plant extract for inhibiting vibrio parahaemolyticus in water environment as well as preparation method and application of composite plant extract

A technology of plant extracts and vibrio hemolyticus, applied in the direction of botany equipment and methods, applications, plant growth regulators, etc., can solve the problems of water algae phase destruction, increased drug resistance of vibrio parahaemolyticus, and increased costs

Active Publication Date: 2021-04-09
清远海贝生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for shrimp farming models with obvious salinity differences, such as earthen ponds and high-level ponds, the regular use of compliant disinfectants and the use of beneficial microecological preparations to prevent and control Vibrio parahaemolyticus in water also have some disadvantages, such as Vibrio parahaemolyticus The increase of drug resistance, the cost increase caused by the use of large doses to ensure the effect, and the damage to the algal phase of the water body caused by the use of large doses to ensure the effect, etc. The components of Vibrio hemolyticus have great economic value and social value for promoting health

Method used

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  • Composite plant extract for inhibiting vibrio parahaemolyticus in water environment as well as preparation method and application of composite plant extract
  • Composite plant extract for inhibiting vibrio parahaemolyticus in water environment as well as preparation method and application of composite plant extract
  • Composite plant extract for inhibiting vibrio parahaemolyticus in water environment as well as preparation method and application of composite plant extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] A compound plant extract for inhibiting Vibrio parahaemolyticus in the water environment. In terms of weight percentage, we weighed 45% of the extract of Dragon Ball Grass, 38% of the extract of Gangmei, 9% of the extract of Vibrio parahaemolyticus, and the extract of Dandelion 8%. Under the environment of 28°C, the above-mentioned components are mixed, and the conventional ultrasonic emulsification is uniform, and the obtained mixed solution is the compound plant extract.

[0076] Among them, the above-mentioned extract of Dragon Ball Grass is obtained by the following method: cut short Dragon Ball Grass, bake at 47°C for 8 hours, pass through a 20-mesh sieve to obtain a crushed product, and take 100 parts of Dragon Ball Grass pulverization, mix well with 1000 parts of water, then add 12000IU cellulase and papain complex, in 38°C environment, airtight, interval stirring, store for 68-72h, unpack, add 500 parts of water, add spores in liquid form 15 copies of culture m...

experiment example 1

[0080] Experimental Example 1 Composite Plant Extract of the present invention compares the inhibitory effect of common aquatic pathogenic bacteria

[0081] 1. Test material

[0082] 1.1 Test strains

[0083] Vibrio parahaemolyticus, Vibrio alginolyticus, Vibrio harveyi, Aeromonas hydrophila: all are preserved and provided by our research group.

[0084] Potassium persulfate: Potassium persulfate with a content of 50%.

[0085] 1.2 Test consumables

[0086] TSB medium, Petri dishes, test tubes, distilled water, etc.

[0087] 1.3 Compound plant extracts

[0088] The compound plant extract of the present invention used in this experiment, its preparation method is the same as embodiment 1.

[0089] 2. Test method

[0090] 2.1 Bacterial suspension preparation

[0091] Inoculate Vibrio parahaemolyticus, Vibrio alginolyticus and Vibrio harveii respectively into TSB nutrient broth containing 0.5% sodium chloride, culture at 28°C for 16-20h, collect the bacteria by centrifugat...

experiment example 2

[0110] Experimental Example 2 Inhibition of Composite Plant Extract Composition of the present invention on Vibrio parahaemolyticus

[0111] 1. Test material

[0112] 1.1 Preparation of compound plant extracts

[0113] The composite plant extract of the present invention used in this experiment is named as Y, and its preparation method is the same as in Example 1.

[0114] 1.2 Preparation of compound plant extracts with missing prescriptions

[0115] Based on the compound plant extract formula prepared in Example 1 of the present invention, through the technical idea of ​​one-component deletion or two-component simultaneous deletion or three-component simultaneous deletion, and the missing components are replaced by PBS in equal amounts, preparing a compound plant extract with a single deletion of Dragon Ball extract, a compound plant extract with a single deletion of Plum extract, a compound plant extract with a single deletion of C. Preparation of the compound plant extra...

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Abstract

The invention belongs to the technical field of water environment bacteriostatic agents, and discloses a composite plant extract for water environment vibrio parahaemolyticus, which is mainly prepared from the following components in percentage by mass: 35%-55% of a gerbera piloselloides extract, 30%-42% of a roughhaired holly extract, 5%-12% of a gynura bicolor flower extract and 5-12% of a dandelion extract. Compared with traditional GMP disinfectants, the compound plant extract belongs to an environment-friendly substance, is large in the safety concentration range of shrimps, and has an obvious inhibiting effect on vibrio parahaemolyticus when being used for splashing chemical water and cannot be interfered by organic matters in water; when the use dosage is smaller than or equal to 5 mg / L, abnormal propagation of vibrio parahaemolyticus in upper-layer, middle-layer and bottom-layer culture of a culture water body can be effectively inhibited, and the safety to pelteobagrus fulvidraco, ictalurus punctatus and silurus meridionalis is good.

Description

technical field [0001] The invention relates to the technical field of antibacterial agents for water environment, and more specifically, relates to a compound plant extract for inhibiting Vibrio parahaemolyticus in water environment, its preparation method and application. Background technique [0002] Vibrio parahaemolyticus is one of the members of pathogenic Vibrio, Gram-negative bacteria, Vibrio family Vibrio genus, mostly arc-shaped, rod-shaped or oval-shaped under the electron microscope, with flagella and capsule, without Spores are widely distributed in saline water bodies such as seawater and saltwater lakes. Vibrio parahaemolyticus grows well on a medium with a salt content of 3%, and does not grow without salt, so it is also called halophilic bacteria. The optimum growth temperature of this bacterium is 37°C, so summer is the season with frequent occurrence of diseases caused by this bacterium. Practice has shown that in high temperature seasons, Vibrio parahaem...

Claims

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Application Information

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IPC IPC(8): A01N65/34A01N65/12A01N65/08A01N63/22C02F1/50C02F103/20
CPCA01N65/00A01N65/08A01N65/12A01N65/34A01N63/22C02F1/50C02F2103/20C02F2303/04
Inventor 黄锦炉
Owner 清远海贝生物技术有限公司
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