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A compound plant extract for inhibiting vibrio parahaemolyticus in water environment and its preparation method and application

A plant extract, Vibrio haemolyticus technology, applied in the directions of botanical equipment and methods, applications, chemical instruments and methods, etc., can solve the problems of increased drug resistance of Vibrio parahaemolyticus, destruction of algal phases in water bodies, and increased cost.

Active Publication Date: 2021-08-03
清远海贝生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for shrimp farming models with obvious salinity differences, such as earthen ponds and high-level ponds, the regular use of compliant disinfectants and the use of beneficial microecological preparations to prevent and control Vibrio parahaemolyticus in water also have some disadvantages, such as Vibrio parahaemolyticus The increase of drug resistance, the cost increase caused by the use of large doses to ensure the effect, and the damage to the algal phase of the water body caused by the use of large doses to ensure the effect, etc. The components of Vibrio hemolyticus have great economic value and social value for promoting health

Method used

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  • A compound plant extract for inhibiting vibrio parahaemolyticus in water environment and its preparation method and application
  • A compound plant extract for inhibiting vibrio parahaemolyticus in water environment and its preparation method and application
  • A compound plant extract for inhibiting vibrio parahaemolyticus in water environment and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] A compound plant extract for inhibiting Vibrio parahaemolyticus in the water environment. In terms of weight percentage, we weighed 45% of the extract of Dragon Ball Grass, 38% of the extract of Gangmei, 9% of the extract of Vibrio parahaemolyticus, and the extract of Dandelion 8%. Under the environment of 28°C, the above-mentioned components are mixed, and the conventional ultrasonic emulsification is uniform, and the obtained mixed solution is the compound plant extract.

[0076] Among them, the above-mentioned extract of Dragon Ball Grass is obtained by the following method: cut short Dragon Ball Grass, bake at 47°C for 8 hours, pass through a 20-mesh sieve to obtain a crushed product, and take 100 parts of Dragon Ball Grass pulverization, mix well with 1000 parts of water, then add 12000IU cellulase and papain complex, in 38°C environment, airtight, interval stirring, store for 68-72h, unpack, add 500 parts of water, add spores in liquid form 15 copies of culture m...

experiment example 1

[0080] Experimental Example 1 Composite Plant Extract of the present invention compares the inhibitory effect of common aquatic pathogenic bacteria

[0081] 1. Test material

[0082] 1.1 Test strains

[0083] Vibrio parahaemolyticus, Vibrio alginolyticus, Vibrio harveyi, Aeromonas hydrophila: all are preserved and provided by our research group.

[0084] Potassium persulfate: Potassium persulfate with a content of 50%.

[0085] 1.2 Test consumables

[0086] TSB medium, Petri dishes, test tubes, distilled water, etc.

[0087] 1.3 Compound plant extracts

[0088] The compound plant extract of the present invention used in this experiment, its preparation method is the same as embodiment 1.

[0089] 2. Test method

[0090] 2.1 Bacterial suspension preparation

[0091] Inoculate Vibrio parahaemolyticus, Vibrio alginolyticus and Vibrio harveii respectively into TSB nutrient broth containing 0.5% sodium chloride, culture at 28°C for 16-20h, collect the bacteria by centrifugat...

experiment example 2

[0110] Experimental Example 2 Inhibition of Composite Plant Extract Composition of the present invention on Vibrio parahaemolyticus

[0111] 1. Test material

[0112] 1.1 Preparation of compound plant extracts

[0113] The composite plant extract of the present invention used in this experiment is named as Y, and its preparation method is the same as in Example 1.

[0114] 1.2 Preparation of compound plant extracts with missing prescriptions

[0115] Based on the compound plant extract formula prepared in Example 1 of the present invention, through the technical idea of ​​one-component deletion or two-component simultaneous deletion or three-component simultaneous deletion, and the missing components are replaced by PBS in equal amounts, preparing a compound plant extract with a single deletion of Dragon Ball extract, a compound plant extract with a single deletion of Plum extract, a compound plant extract with a single deletion of C. Preparation of the compound plant extra...

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Abstract

The invention belongs to the technical field of antibacterial agents for water environment, and discloses a compound plant extract for Vibrio parahaemolyticus in water environment. The compound plant extract is mainly made of the following components by mass percentage: Dendrobium chinensis extract 35%‑55%, 30%‑42% of plum extract, 5%‑12% of dandelion extract, 5‑12% of dandelion extract. Compared with the safety of the traditional GMP disinfectant, the compound plant extract of the present invention belongs to the environment-friendly substance, and the safe concentration range of the prawn body is large. It will be disturbed by organic matter in the water body. When the dose is ≤5mg / L, it can effectively inhibit the abnormal reproduction of Vibrio parahaemolyticus in the upper, middle and bottom layers of the aquaculture water body, and it is safe for yellow catfish, channel catfish and channel catfish good.

Description

technical field [0001] The invention relates to the technical field of antibacterial agents for water environment, and more specifically, relates to a compound plant extract for inhibiting Vibrio parahaemolyticus in water environment, its preparation method and application. Background technique [0002] Vibrio parahaemolyticus is one of the members of pathogenic Vibrio, Gram-negative bacteria, Vibrio family Vibrio genus, mostly arc-shaped, rod-shaped or oval-shaped under the electron microscope, with flagella and capsule, without Spores are widely distributed in saline water bodies such as seawater and saltwater lakes. Vibrio parahaemolyticus grows well on a medium with a salt content of 3%, and does not grow without salt, so it is also called halophilic bacteria. The optimum growth temperature of this bacterium is 37°C, so summer is the season with frequent occurrence of diseases caused by this bacterium. Practice has shown that in high temperature seasons, Vibrio parahaem...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N65/34A01N65/12A01N65/08A01N63/22C02F1/50C02F103/20
CPCA01N65/00A01N65/08A01N65/12A01N65/34A01N63/22C02F1/50C02F2103/20C02F2303/04
Inventor 黄锦炉
Owner 清远海贝生物技术有限公司
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