Ainsliaea fragrans champ caffeoylquinic acid extracts and preparation and application thereof

A technology of caffeoyl quinic acid and caffeoyl quinic acid, which is applied in the field of traditional Chinese medicine, can solve the problems of no cervicitis and research on the inhibitory effect of drug-resistant bacteria, and achieve good anti-infection effect, improvement of diseased tissue lesions, and avoidance of drain effect

Active Publication Date: 2011-06-22
江西沐恩堂生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, the above patent research shows that the extract of apricot rabbit ear wind has anti-inflammatory and antibacterial effects, but there is no research involving its inhibitory effect on cervicitis and drug-resistant bacteria

Method used

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  • Ainsliaea fragrans champ caffeoylquinic acid extracts and preparation and application thereof
  • Ainsliaea fragrans champ caffeoylquinic acid extracts and preparation and application thereof
  • Ainsliaea fragrans champ caffeoylquinic acid extracts and preparation and application thereof

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preparation example Construction

[0036] Preparation of reference solution

[0037] Accurately weigh the appropriate amount of 3-caffeoylquinic acid, 3,5-dicaffeoylquinic acid, and reference substance, add 50% ethanol to make each 1 mL contain 3-caffeoylquinic acid 0.034mg / mL, 3, 5-dicaffeoylquinic acid 0.0425mg / mL mixed reference solution, set aside. Refer to the attachment for the HPLC results of the reference substance figure 2 .

[0038] According to above-mentioned chromatographic condition, sample is measured, and its HPLC result sees attached image 3 .

Embodiment 1

[0040] Take 1000g of dried medicinal material of Xingxiang rabbit ear wind, cut off (2~5cm), use 10 times of 50% ethanol, heat and reflux at 85°C to extract 3 times, filter, combine to obtain extract A, concentrate under reduced pressure at 60°C to extract A 1 / 6 times of the concentrated solution B is obtained. The concentrated solution B was adjusted to pH 2 with dilute hydrochloric acid, the acidic concentrated solution B was treated with macroporous adsorption resin AB-8, the resin was first washed with deionized water, eluted with 20% ethanol for 2 column volumes, and then washed with Elute 10 column volumes with 50% ethanol, collect the eluate with 50% ethanol concentration, concentrate under reduced pressure at 60°C to remove alcohol, and obtain concentrated solution C, which is then adjusted to pH 2 with dilute hydrochloric acid, and passed through macroporous adsorption resin For HPD-826, rinse the resin with deionized water first, then elute with water-ethanol gradien...

Embodiment 2

[0042] Xingxiang rabbit ear wind dried medicinal material 1000g, cut off (2~5cm), extracted 3 times with 15 times of 60% ethanol, heated and refluxed at 85°C, filtered, combined to obtain extract A, concentrated under reduced pressure at 60°C to extract A 1 / 7 times to obtain the concentrate B. The concentrated solution was adjusted to pH 1 with dilute hydrochloric acid B, and the acidic concentrated solution B was treated with macroporous adsorption resin HPD-826, and the resin was first washed with deionized water, eluted with 30% ethanol for 2 column volumes, and then washed with 8 column volumes were eluted with 60% ethanol, and the eluate with an ethanol concentration of 60% was collected and concentrated under reduced pressure at 60°C to obtain a concentrated solution C. 826, first wash the resin with deionized water, then sequentially elute with water-ethanol, collect eluents with an ethanol concentration of 60%, combine them, and dry them in vacuum to obtain 16g of apri...

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Abstract

The invention relates to ainsliaea fragrans champ caffeoylquinic acid extracts and preparation and application thereof. The ainsliaea fragrans champ caffeoylquinic acid extracts are prepared by the following steps of: performing refluxing extraction of the ainsliaea fragrans champ by using ethanol, filtering, concentrating and removing ethanol, adjusting the pH value to 1 to 3 by adding hydrochloric acid, applying macroporous adsorbent resin, water-washing, adding ethanol for gradient elution, and collecting eluent, of which the ethanol concentration is 50 to 70 percent; performing condensation and ethanol removal, adjusting the pH value to 1 to 3 by adding hydrochloric acid, using macroporous adsorbent resin, adding water and ethanol for gradient elution respectively, and collecting the eluent, of which the ethanol concentration is 40 to 70 percent; mixing the eluent, and vacuum drying the eluent to obtain the extracts. The UV content of the ainsliaea fragrans champ caffeoylquinic acid extracts is over 70 percent, the HPLC content is over 50 percent, and the content of 3-caffeoylquinic acid and the content of 3,5-dicaffeoylquinic acid are both over 10 percent. The ainsliaea fragrans champ caffeoylquinic acid extracts have the effects of resisting inflammations, bacteria, medicament-resistant strains, well improves pathologically changed tissues of rat suffering from cervicitis, obviously reduces the uterus index and the number of leukocytes and can be independently prepared into the medicaments for treating cervicitis or combined with other medicaments to prepare the medicaments for treating cervicitis.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine, and relates to a preparation process of caffeoylquinic acid extract of apricot rabbit ear wind and its application in the development of anti-inflammation, anti-bacteria, anti-drug-resistant strains, and treatment of cervicitis and other novel drugs. Background technique [0002] Apricot Rabbit Ears ( Ainsliaea fragrans Champ. ) is a plant of the genus Rabbit's ear in the family Asteraceae, which is included in the 1996 edition of "Jiangxi Provincial Standards for Chinese Medicinal Materials". It has the effects of clearing away heat and detoxification, promoting dampness, and stopping bleeding. The whole herb is used as medicine in the folk. bite. Distributed in Jiangsu, Zhejiang, Jiangxi, Fujian and other places. Modern pharmacological experiments have shown that the main medicinal active ingredients in Xingxiang rabbit ear wind mainly contain 3-caffeoyl quinic acid, 3,5-dicaffeoy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/28A61P15/00A61P29/00A61P31/04
Inventor 杨世林罗晓健张武岗魏慧珍陈兰英孙勇兵黄恺
Owner 江西沐恩堂生物科技有限公司
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