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Method for evaluating unique nitrosamine inhibiting function of additive on tobacco

A technology of inhibition and additives, applied in the field of tobacco harm reduction, can solve the problems of low efficiency, cumbersome process, unsatisfactory reproducibility, etc.

Active Publication Date: 2009-11-04
ZHENGZHOU TOBACCO RES INST OF CNTC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is aimed at the defects of cumbersome process, low efficiency and unsatisfactory reproducibility by directly measuring the change of flue gas TSNAs to evaluate the inhibitory effect of additives in the above-mentioned prior art, and a fast and accurate evaluation method specially established method, to evaluate the effect of additives on reducing TSNAs, so as to conduct a preliminary screening of different additives to be applied to cigarettes

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0022] Mix nicotine-ethanol solution (nicotine concentration 1 mmol / L) and nitrite-water solution (nitrite concentration 10%) in 10 mL of citric acid at pH 4.6 according to nicotine: nitrite = 1:5 - in disodium hydrogen phosphate buffer solution, heated and stirred at 90° C. for 3 h. The reaction solution was extracted with 20 mL×3 dichloromethane (chromatographically pure), the dichloromethane extracts were combined, and dried over anhydrous sodium sulfate. filter. After adding 100 μL of internal standard solution, concentrate and dilute to 1 mL. Adopt GC-TEA method (capillary gas chromatography conditions: chromatographic column HP-50+30m×0.53mm×1μm; pre-column guard column 1m×0.53mm (empty column tube); temperature program, initial temperature 150°C, keep 2min, 2 ℃ / min rises to 175°C, maintains for 8 minutes, rises to 230°C at 3°C / min, rises to 250°C at 30°C / min, and holds for 5 minutes; the temperature of the injection port is 230°C; the carrier gas is helium, and the co...

example 2

[0024] Mix nicotine-ethanol solution (nicotine concentration 1 mmol / L) and nitrite-water solution (nitrite concentration 10%) in 10 mL of citric acid at pH 4.6 according to nicotine: nitrite = 1:5 - in disodium hydrogen phosphate buffer solution, heated and stirred at 70°C for 5h. The reaction solution was extracted with 20 mL×3 dichloromethane (chromatographically pure), the dichloromethane extracts were combined, and dried over anhydrous sodium sulfate. filter. Concentrate after adding the internal standard, and dilute to 1 mL. The content of NNN and NNK in the system was determined by GC-TEA method. Parallel experiments were repeated 5 times. The results show that the stability of the model system is good, and the relative standard deviation of the test results is less than 10%.

example 3

[0026] Mix nicotine-ethanol solution (nicotine concentration 1 mmol / L) and nitrite-water solution (nitrite concentration 10%) in 40 mL of citric acid at pH 4.6 according to nicotine: nitrite = 1:5 - In the disodium hydrogen phosphate buffer solution, under the conditions of not adding vitamin C (dissolved in citric acid-disodium hydrogen phosphate buffer solution) or propylene glycol, heating and stirring at 90° C. for 3 hours, respectively. The reaction solution was extracted with 20 mL×3 dichloromethane (chromatographically pure), the dichloromethane extracts were combined, and dried over anhydrous sodium sulfate. filter. Concentrate after adding the internal standard, and dilute to 1 mL. The contents of NNN and NNK in the blank system and the inhibited system were determined by GC-TEA method, and the inhibitory effects of vitamin C or propylene glycol were investigated. The results showed that vitamin C had an inhibitory effect on the production of TSNAs, and the inhibiti...

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Abstract

The invention provides a method for evaluating the unique nitrosamine inhibiting function of additive on tobacco. The method comprises the following steps: a, blank system reaction, in which, nicotine and nitrite are mixed in buffer solution according to a molar ratio of 1:1-5, and react under a certain condition; b, inhibiting system reaction, in which, the nicotine and the nitrite are mixed according to the same proportion of the step a, after additive in certain amount is added to the mixture, and the mixture perform reaction according to the same buffer system, the same temperature and time of the step a; and c, respective extraction and separation, measuring TSNAs contents in the blank system and the inhibiting system respectively by a GC-TEA method to compare differences of the two systems and evaluate the inhibiting effects of the additive. The method has the advantages that a quick, accurate and scientific evaluation method is established, and the inhibiting effect of various additives on TSNAs generation can be objectively evaluated through specific measurement data, so that initial screen can be carried out on different additives which are planned to apply to tobacco.

Description

technical field [0001] The invention relates to the field of tobacco harm reduction, in particular to a method for evaluating the inhibitory effect of additives on tobacco-specific nitrosamines. The method can be used to evaluate the effect of additives on reducing tobacco-specific nitrosamines (TSNAs), so as to be applied to tobacco-specific nitrosamines (TSNAs). Preliminary screening of different additives in cigarettes. Background technique [0002] Smoking and health issues are one of the major challenges faced by the tobacco industry during its development. The tobacco industry has paid attention to smoking and health issues since the 1970s. After the 1980s, the reduction of harmful components such as tar was taken as the main research topic, and various technical researches were carried out, and significant progress was made. [0003] The main harmful substances in the flue gas are: carbon monoxide, nitrogen oxides, acrolein and other aldehydes in the gas phase of the...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N1/28G01N30/06G01N30/30G01N30/74
Inventor 茹呈杰刘珊刘克建范坚强黄华杨振民赵明月
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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