14 results about "Nicotine concentration" patented technology

This invention discloses a nicotine-resistant pectin lyase mutant and its application in tobacco leaf treatment. Using pectin lyase PL6 as the starting enzyme, this invention designs and optimizes mutation sites, designing single-site saturation mutations based on the two hydrogen bond formation sites between the enzyme and nicotine, Lys-107 and Asp-84, to construct mutants. The pectin lyase mutants D84A and K107R of this invention retain 83.63% and 81.30% of their enzyme activities at a 1% nicotine concentration, respectively. Spraying tobacco leaves with D84A and K107R enzyme solutions significantly improves tobacco leaf quality due to pectin degradation.

The invention belongs to the field of nicotine content testing, and particularly relates to an electronic cigarette cartridge and a method for detecting the nicotine content in electronic cigarette liquid. The detection method comprises the following steps: (1) preparing a series of standard working solutions containing a solvent and nicotine with different contents, taking a Raman intensity ratio of a nicotine characteristic peak to a solvent characteristic peak as a relative Raman intensity, carrying out linear fitting on the relative Raman intensity and a nicotine concentration, and establishing a standard curve; and (2) on the premise of not breaking the electronic cigarette cartridge, directly carrying out Raman spectrum test on the electronic cigarette liquid in the electronic cigarette cartridge, and quantifying the nicotine content of the electronic cigarette liquid according to the standard curve. According to the method, nondestructive testing is carried out on the premise that the electronic cigarette cartridge is not broken, the consistency with the detection result of a GB41701-2022 gas chromatography method is good, and it is indicated that the method can meet the actual detection result requirement in the aspect of the detection result accuracy.

The invention provides a domestication method of a nicotine degrading strain, which is characterized by comprising the following steps: S1, providing a starting strain: acquiring a Sphingobacterium psychromonas strain or a flora containing the Sphingobacterium psychromonas strain, and preparing a bacterial solution; s2, strain domestication: inoculating the bacterial liquid into a domestication culture medium for domestication, gradually increasing the concentration of nicotine in the domestication culture medium in a gradient manner, gradually reducing the concentration of a yeast extract in a gradient manner, and domesticating for 2-20 generations to obtain a domesticated strain. The strain obtained by the method has the capability of efficiently degrading nicotine, can survive in high-concentration nicotine wastewater, can degrade organic matters in the wastewater, plays a dual role, and has a relatively high industrial value.

The present invention relates to a structure for a biosensor, a biosensor including the same, and a method for real-time nicotine analysis in saliva using the biosensor. According to the present invention, a structure connected to an electrode of a biosensor forms a structure of a crystalline carbon membrane / calcium ion-selective membrane / TRPA1-containing nanovesicle. When nicotine is contained in saliva containing calcium ions, the nanovesicle having a TRPA1 ion channel in the outermost layer of the structure specifically reacts with the nicotine contained in the saliva to introduce calcium ions. The resulting change in calcium ion concentration changes the surface potential of the crystalline carbon membrane in contact with the calcium ion-selective membrane through the calcium ion-selective membrane. By measuring the change in electrical conductivity, such as the change in open-circuit voltage, nicotine can be detected even in minute amounts of 1 pM, thereby allowing for the quantitative determination of the nicotine concentration in a smoker's saliva in real time.

The application discloses Au@PATP@Ag NRs nanomaterial and a preparation method and application thereof. The application also discloses a method for qualitatively and quantitatively detecting nicotine concentration based on surface enhanced Raman scattering technology and application thereof. The Au@PATP@Ag NRs nanoparticles are used as active base materials of SERS, and a target object is analyzed and detected by using a Raman spectrometer. According to a linear relation equation of a ratio of SERS signal intensity of different concentrations of nicotine solution to characteristic peaks of nicotine and an internal standard substance, the content of nicotine in a solution to be detected can be obtained, and the detection sensitivity of nicotine is greatly improved. The internal standard molecule is introduced into the core-shell structure, and the accuracy of quantitative detection of nicotine is improved. The application also has great application in sensitive detection of nicotine in cigarettes and environmental cigarette smoke. In addition, the application also has the advantages of simple principle, rapidness, simple pretreatment, high selectivity, high detection sensitivity and the like.

The invention belongs to the technical field of tobacco strains, and particularly relates to a rhodotorula mucilaginosa H04 strain and application thereof in preparation of tobacco flavor. The rhodotorula mucilaginosa H04 provided by the invention has excellent aroma producing performance and can produce tobacco characteristic aroma substances such as alcohols, aldehydes, esters and pyrazines at high yield. The rhodotorula mucilaginosa H04 has good tolerance to nicotine, can still keep high growth activity under the condition that the nicotine concentration is as high as 8 mg / mL, and is suitable for being used for preparing tobacco flavor; the tobacco flavor prepared by the invention contains a plurality of natural aroma components, has good coordination with the original aroma of tobacco, and can significantly improve the aroma richness and comfort of tobacco products; the highest beta-glucosidase activity of the rhodotorula mucilaginosa H04 reaches 464.0 + / -24.4 U / mL, and the laccase activity reaches 60.4 + / -3.2 U / mL, so that the release of bound aroma precursor substances in tobacco is facilitated, and the aroma expression is enhanced.

The utility model discloses an atomizing core and electronic cigarette of adjustable cool degree, sweetness and nicotine concentration, through setting up two heating elements in a warehouse, and make two heating elements connect with main control unit respectively, form different two independent electric circuits respectively, again through the different work power of two heating bodies to provide different working temperature, make cool flavor agent, sweetening agent and nicotine be atomized in corresponding temperature range to different degree, thereby change cool flavor agent, sweetening agent and the percentage content of nicotine atomized in unit time namely atomization rate, realize the adjustment to cool degree, sweetness and nicotine concentration, and do not need to increase too many complex structures, also can not waste too much product's internal use space, make the structure of whole product simple, the assembly process is few, satisfy the customer demand simultaneously, also be favorable to the cost saving.

This application provides an atomizing liquid and its preparation method, wherein the atomizing liquid comprises the following components in parts by weight: 50-68 parts distilled water; 10-20 parts propylene glycol; 7-8 parts nicotine salt; 20-28 parts water-soluble flavoring; 0.5-2 parts humectant; 0.15-0.5 parts cooling agent; 0.8-1.5 parts sweetener; and 0.05-0.15 parts pH adjuster. The atomizing liquid and its preparation method provided in this application, comprising specific parts by weight of distilled water, propylene glycol, nicotine salt, and other components, effectively alleviate the burning and dryness in the throat caused by high nicotine concentrations by optimizing the formula ratio and preparation process. It has the advantages of reducing throat irritation and improving user comfort at high nicotine concentrations.

The invention belongs to the technical field of microbial pesticides, and particularly relates to bacillus thuringiensis with high-concentration nicotine tolerance and application of the bacillus thuringiensis in tobacco waste treatment. The bacillus thuringiensis capable of tolerating nicotine is obtained from the original strain through repeated domestication and screening, the tolerated total nicotine concentration exceeds 1.26 mg / ml and is 3 times that of the original strain, the content of tobacco waste in a fermentation substrate is greatly increased, the obtained fermentation culture does not need to be further treated, and the production cost is reduced. The compound can be directly used as a biocontrol agent for lepidoptera and hemiptera pests, can also be mixed with nicotine botanical pesticides, and is suitable for large-area popularization and application.

A method for recovering concentrated nicotine from cured tobacco includes collecting a first aqueous solution comprising moisture released from cured tobacco and comprising nicotine. The first aqueous solution is passed through a strong cation resin to sorb the nicotine. A second aqueous solution comprising ammonium hydroxide is passed through the resin to elute the nicotine and form a third aqueous solution comprising nicotine and ammonium hydroxide. The ammonium hydroxide is removed from the third aqueous solution to generate a fourth aqueous solution comprising nicotine. The fourth aqueous solution is incubated under conditions to cause the fourth aqueous solution to separate into a fifth aqueous solution comprising a first nicotine concentration and a sixth aqueous solution comprising a second nicotine concentration. The first nicotine concentration is greater than the second nicotine concentration.

The invention relates to preparation of a spore-containing enteric targeted release microsphere and application of the spore-containing enteric targeted release microsphere in nicotine degradation. A probiotic bacillus subtilis 168 strain is subjected to engineering modification, so that a nicotine degrading enzyme is displayed on the surface of a spore of the probiotic bacillus subtilis 168 strain. In order to realize targeted release of intestinal tracts and reduce loss of recombinant spores in a gastric acid environment, the recombinant spores are wrapped by natural polymeric materials sodium hyaluronate and sodium alginate with pH responsiveness, and the spore microspheres with enteric targeted release are prepared. The microspheres greatly protect recombinant spores, effectively realize'interception type degradation 'of nicotine before nicotine is absorbed into blood by intestinal tracts, and continuously degrade nicotine diffused from blood by a'metabolic siphon' effect, so that the concentration of nicotine combined with a central nicotine receptor is remarkably reduced; brain dopamine reward effect induced by smoking is weakened fundamentally, positive feedback circulation of smoking-pleasant sensation is broken effectively, and visceral organs are protected from toxic and side injury of nicotine.

This invention pertains to genetic engineering and relates to an orally administered engineered microbial system and its applications. Through genetic manipulation techniques such as genome integration, a dominant mutant of the nicotine-degrading enzyme Nox is displayed on the surface of Bacillus subtilis spores. This mutant is then coupled with catalase or peroxidase, and genes related to spore germination and the system's own recombinant enzymes are knocked out to construct a strain with self-eliminating resistance genes. This invention utilizes orally administered engineered bacteria to colonize the intestines. The "molecular degradation machine and metabolic siphon" effect produced by the engineered bacteria continuously degrades nicotine diffused from the bloodstream, thereby significantly reducing the concentration of nicotine binding to central nicotine receptors. This fundamentally weakens the dopamine reward effect induced by smoking, effectively breaking the positive feedback loop of "smoking-pleasure," and providing a new method for treating nicotine dependence. As a "plug-and-play" platform technology, displaying different degradation enzymes on the surface of Bacillus subtilis spores can also degrade other harmful substances.

The invention relates to a container (1) comprising a composition with a nicotine concentration less than or equal to 20 milligrams per milliliter characterized in that said container (1) takes the form of a long, narrow sachet (1) of which: i) the two opposite ends each have at least one weld (2,3) and ii) the weld (2), at at least one of these two ends, is not perpendicular to the long edges of said long, narrow sachet but on the contrary allows said end to have a narrowed section (4).

The invention relates to the technical field of electronic cigarettes, and discloses a personalized service method based on user behavior time sequence data, a computer readable storage medium and a computer program product. The method comprises the steps of anchoring a plurality of smoke cartridges stably used by a user in recent M days according to behavior time sequence data of electronic cigarette smoking of the user, and analyzing a current nicotine harm reduction process of the user; if the user is in the harm reduction stable period at present, marking a'harm reduction stable period 'label for the user, and when it is monitored that the nicotine concentration of the smoke cartridge stably used by the user reaches a preset minimum threshold value, pushing an exclusive coupon of the smoke cartridge without the nicotine version to the user. According to the electronic cigarette smoking behavior time sequence data of the user, the current nicotine harm reduction process of the user is automatically quantified, and after the user is in the harm reduction stable period, the user is induced to zero nicotine in a mode of pushing coupons to the user, so that the user is scientifically assisted to achieve the harm reduction target.