Biological composite oxygen producer and preparation method thereof

A composite oxygenate and oxygenate technology, which is applied in chemical instruments and methods, biological water/sewage treatment, water/sludge/sewage treatment, etc., can solve the problem of non-decomposition of oxygen-consuming substances at the bottom and the effect of probiotics Can not be reflected, the chemical oxygen consumption can not be reduced and other problems, to achieve the effect of alleviating the lack of oxygen in the pond, improving the micro-ecological balance, and increasing the oxygen effect

Active Publication Date: 2015-08-12
山东宝来利来生物工程股份有限公司
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this way, the bacterial liquid cannot reach the bottom of the pond by wind force or its own gravity, the oxygen-consuming substances at the bottom cannot be decomposed, the chemical oxygen consumption cannot be reduced, and the problem of hypoxia at the bottom cannot be fundamentally solved. Strength alleviates the problem of hypoxia, and the effect of probiotics is not reflected
If chemical oxygenates and probiotics are simply compounded and used, there may be an inhibitory effect between the chemical oxygenates and probiotics, and it is difficult for the two to achieve a synergistic effect

Method used

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  • Biological composite oxygen producer and preparation method thereof
  • Biological composite oxygen producer and preparation method thereof
  • Biological composite oxygen producer and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1: Screening and preparation of chemical oxygenates

[0046] 1. Materials and methods

[0047] 1.1 Materials:

[0048] Reagents: anhydrous sodium carbonate, anhydrous sodium sulfate, 30% hydrogen peroxide, sodium silicate, salicylic acid, sodium tripolyphosphate, sodium chloride, stearic acid, polyethylene glycol 4000, carbon tetrachloride, Sodium percarbonate, the above reagents were purchased from Tianjin Kaitong Chemical Reagent Co., Ltd.; oxygen source, an oxygen-enhancing product produced by Shandong Baolai Lilai Bioengineering Co., Ltd.

[0049] Instruments: Portable Dissolved Oxygen Meter (Leici JPB-607A), Water Bath, Breeding Glass Cylinder (Barrel), Magnetic Stirrer, Watering Can.

[0050] 1.2 Method

[0051] 1.2.1 Preparation of basic oxygenate

[0052] ①The preparation of sodium percarbonate:

[0053] Spray 30% hydrogen peroxide evenly on anhydrous sodium carbonate in different proportions by spraying, and dry naturally in a ventilated place t...

Embodiment 2

[0083] The screening of embodiment 2 degrading ammonia nitrogen probiotics

[0084] 1 test material

[0085] Strains: 117 strains of Bacillus were provided by Shandong Baolai Lilai Bioengineering Co., Ltd. Institute of Biology;

[0086] Bacillus culture medium: 0.2% glucose, 1% peptone, 0.5% sodium chloride, 0.5% yeast extract, pH 7.0; all are percentages by mass;

[0087] Synthetic aquaculture wastewater: glucose 0.1g / L, K 2 HPO 4 0.75g / L, NaH 2 PO 4 0.25g / L, NaHCO 3 1g / L, MgSO 4 0.02g / L, NH 4 Cl 100mg / L (can be adjusted as needed), NaNO 2 100mg / L (can be adjusted as needed);

[0088] Breeding wastewater: glass tank fish farming wastewater, add NH 4 Cl 20mg / L, NaNO 2 10mg / L;

[0089] Sodium citrate was purchased from Tianjin Kaitong Chemical Reagent Co., Ltd.

[0090] 2 test method

[0091] 2.1 Determination of ammonia nitrogen - Nessler's reagent colorimetric method

[0092] 2.1.1 Reagents:

[0093] (1) Ammonium standard stock solution: Weigh 3.819g of ...

Embodiment 3

[0126] Embodiment 3: the preparation of biocomposite oxygenate

[0127] Formula: 2 parts of chemical oxygen enhancer, 1 part of biological agent, 0.1 part of sodium citrate, 0.8 part of adhesive;

[0128] The biological agent is bacterial powder of Bacillus subtilis N9-1-35 (Bacillus subtilis N9-1-35);

[0129] The binder is a paste obtained by mixing starch and water at a ratio of 1g:4mL.

[0130] Preparation:

[0131] (1) Preparation of chemical oxygenate: Spray hydrogen peroxide evenly on anhydrous sodium carbonate, stir continuously during this period, then add sodium chloride and mix well to form a crystalline compound, and grind the compound into powder after cooling. Promptly get the chemical oxygenation agent; the mass ratio of anhydrous sodium carbonate and hydrogen peroxide addition is 5:3, and the addition of sodium chloride is 10% of anhydrous sodium carbonate and hydrogen peroxide gross weight;

[0132] (2) Mix the chemical oxygenate prepared in step (1), the b...

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Abstract

The invention discloses a biological composite oxygen producer, which is prepared from the following raw materials by weight: 1.5-3 parts of a chemical oxygen producer, 1 part of a biological agent, 0.1-0.2 parts of sodium citrate and 0.6-1 part of an adhesive. The chemical oxygen producer is prepared by mixing anhydrous sodium carbonate and hydrogen peroxide in the mass ratio of 5: 3, and adding sodium chloride in the mixing process. The biological agent is a mixed powder of one or two of Bacillus subtilis N9-1-35 and Bacillus subtilis B7348 at an arbitrary ratio. The invention also discloses a preparation method of the biological composite oxygen producer. The biological composite oxygen producer provided by the invention effectively combines the biological agent with the chemical oxygen producer, so that the biological agent and oxygen producer can reach the bottom of the pond at the same time; the oxygen producer releases oxygen to increase the dissolved oxygen at the bottom, and the biological agent can decompose harmful materials at the bottom, degrade ammonia content and play a role in regulating water quality and balancing the microecology of the water, so as to fundamentally ease the problem of pond hypoxia.

Description

technical field [0001] The invention relates to a biological composite oxygenate and a preparation method thereof. Background technique [0002] The dissolved oxygen in natural water is generally in the range of 8-12mg / L, which can fully meet the oxygen demand for the normal growth of various fish. However, there are many factors that consume dissolved oxygen in water, such as the respiration of animals and plants, the oxidation and decomposition of organic matter and other reducing substances, etc., which make the dissolved oxygen in water far below the normal value. The problem of dissolved oxygen has become a technical problem in the field of aquaculture. The root cause of water hypoxia is that the breeding density is too high, and the accumulation of residual bait and feces at the bottom of the pond increases the chemical oxygen demand, resulting in insufficient dissolved oxygen at the bottom. The traditional way of increasing oxygen can only increase the dissolved oxyg...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34
Inventor 单宝龙陈雷谷巍郭婷婷王红陈玉春赵倩高绪娜刘姣王志杰彭新利陈静王春凤
Owner 山东宝来利来生物工程股份有限公司
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