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Traditional Chinese medicine composition for relieving inflammatory reaction of osteoarthritis and preparation method thereof

A technology of inflammatory response and composition, applied in the direction of drug combination, pharmaceutical formula, active ingredient of hydroxy compound, etc., can solve the problem of unclear curative effect mechanism, and achieve the effect of exact anti-inflammatory effect and no liver and kidney cytotoxicity

Active Publication Date: 2020-03-06
CAPITALBIO CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problem of adverse reactions of non-steroidal anti-inflammatory drugs, the present invention uses liver and kidney cells to evaluate the cytotoxicity of the developed composition; in view of the problem that the traditional Chinese medicine contains toxic prescriptions and the curative effect mechanism of the traditional Chinese medicine is not clear, the Chinese medicine The composition selects non-toxic medicinal materials in the Pharmacopoeia, and uses transcriptome sequencing to evaluate the expression of key genes in the inflammatory response, and finally screens out a non-toxic and effective composition

Method used

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  • Traditional Chinese medicine composition for relieving inflammatory reaction of osteoarthritis and preparation method thereof
  • Traditional Chinese medicine composition for relieving inflammatory reaction of osteoarthritis and preparation method thereof
  • Traditional Chinese medicine composition for relieving inflammatory reaction of osteoarthritis and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1, preparation and extraction of prescription

[0042] 1) Weigh the prescription to be extracted

[0043] 100 parts of Shenjincao, 100 parts of Sperantophylla, 100 parts of Duhuo, 100 parts of Clematis, 100 parts of Angelica, 100 parts of Borneol, and 100 parts of Sichuan Achyranthes bidentata.

[0044] 2) extract

[0045] Alcohol extraction: use absolute ethanol to prepare an aqueous ethanol solution with a volume percentage of 95%, and prepare an aqueous ethanol solution of 10 mass volume times (g / mL) for each prescription.

[0046] Water extraction: Prepare 10 mass volume times (g / mL) of deionized water for each formulation.

[0047] 3) Put in medicinal materials

[0048] Turn on the power of the Soxhlet extractor, the instrument model is B811. Put the medicinal materials wrapped in filter paper into the bottom of the glass sample tube.

[0049] 4) Put in the extraction reagent

[0050] Pour the extraction solvent (95% by volume ethanol aqueous solut...

Embodiment 2

[0074] Embodiment 2, liver and kidney and chondrocyte toxicity experiment

[0075] 1) Preparation of prescription concentrate:

[0076] Take 50 mg of lyophilized powder prepared in Example 1, add 1 mL of DMSO (alcohol extraction) or 1 mL of deionized water (water extraction) to prepare a 50 mg / mL concentrated solution.

[0077] 2) Configuration of prescription experimental working solution:

[0078] According to the experimental requirements, dilute the medium concentrate to 25 μg / mL, 50 μg / mL, 100 μg / mL, 200 μg / mL, 400 μg / mL working solution.

[0079] 3) Cell plating:

[0080] For the liver cytotoxicity test, use HepG2 cells (human liver cancer cells) as the cell model; for the renal cytotoxicity test, use HK-2 cells (human renal cortical proximal tubule epithelial cells) as the cell model; for the chondrocyte toxicity test, use C28-I2 cells (human normal chondrocytes) were used as cell models.

[0081] Take the culture flasks of two kinds of cells from the incubator, add...

Embodiment 3

[0091] Embodiment 3, the experiment of prescription alleviating the inflammatory reaction of osteoarthritis

[0092] 1. Establishment of osteoarthritis inflammation model:

[0093] (1) Configure human chondrocyte culture medium:

[0094] DMEM / F12 medium, add 10% fetal bovine serum, 50 μm / ml double antibody, 50 μm / ml ascorbic acid.

[0095] (2) Human chondrocyte culture:

[0096] Human chondrocytes (passages 3 to 5) were seeded in 6-well cell culture plates at a seeding density of 2×10 6 Cells / well, human chondrocyte medium was added, and cultured in an incubator for 48 hours.

[0097] (3) Chondrocyte inflammation induction model:

[0098] Add 1ng / ml human recombinant IL-1β to the human chondrocyte culture medium, cultivate in the incubator for 72 hours, and change the medium every 24 hours.

[0099] 2. Cell experiment of prescription drug screening:

[0100] 1) The osteoarthritis cell model successfully induced by inflammation was used for drug screening, and the cells w...

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Abstract

The invention discloses a traditional Chinese medicine composition for relieving inflammatory reaction of osteoarthritis and a preparation method thereof. The traditional Chinese medicine compositionis prepared from the following components in parts by mass: 100-140 parts of common clubmoss herb, 100-140 parts of trberculate speranskia herb, 100-140 parts of doubleteeth pubescent angelica roots,100-140 parts of Chinese clematis roots, 100-140 parts of Chinese angelica, 100-140 parts of borneol and 100-140 parts of medicinal cyathula roots. The traditional Chinese medicine composition does not contain toxic traditional Chinese medicine components indicated in a pharmacopoeia. Cytotoxicity evaluation is carried out on the medicine composition in liver and kidney cells, and according to thecytotoxicity evaluation, the medicine composition has no obvious inhibition effect on activity of liver and kidney cells and can be taken for a long time.

Description

technical field [0001] The invention relates to a traditional Chinese medicine composition for relieving inflammation of osteoarthritis and a preparation method thereof, belonging to the technical field of traditional Chinese medicine preparations. Background technique [0002] Osteoarthritis (OA) is a degenerative joint disease whose incidence increases with age, and its pathological feature is mainly the primary or secondary degeneration of articular cartilage. Relevant surveys show that among the population over 50 years old in my country, the incidence rate of osteoarthritis is about 5%, the incidence rate of women over 60 years old is 25%, and the incidence rate of men is 5%. Cyclooxygenase (COX) can catalyze the synthesis of prostaglandins, which are involved in various pathological and physiological processes in vivo. COX has two isoenzymes, COX-1 and COX-2. The former is related to the normal physiological functions of the body, such as protecting the gastric mucosa...

Claims

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Application Information

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IPC IPC(8): A61K36/716A61P19/02A61K31/045
CPCA61K36/11A61K36/47A61K36/185A61K36/232A61K36/716A61K31/045A61K36/21A61P19/02A61K2236/333A61K2236/331A61K2236/51A61K2300/00
Inventor 黎一江郭弘妍邢婉丽程京
Owner CAPITALBIO CORP
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