Preparation method of magnetic biochar loaded photosynthetic bacteria material and sewage treatment method

A technology for photosynthetic bacteria and sewage treatment, applied in the technical field of coupling nanomaterials and microorganisms, can solve the problems of poor mechanical strength, high water solubility, low porosity, etc., achieve good adsorption and catalytic degradation ability, and improve microbial degradation Activity, effect of promoting microbial growth

Active Publication Date: 2016-11-16
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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Problems solved by technology

These carrier materials have high water solubility, poor stability, and poor mechanical strength, which limit the practical application of immobilized microorganisms in sewage treatment.
Other porous materials used as carriers such as activated carbon, zeolite, ceramic balls, quartz sand, etc. have their own characteristics, but they have disadvantages such as low porosity, small specific surface area, and large specific area.

Method used

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  • Preparation method of magnetic biochar loaded photosynthetic bacteria material and sewage treatment method
  • Preparation method of magnetic biochar loaded photosynthetic bacteria material and sewage treatment method
  • Preparation method of magnetic biochar loaded photosynthetic bacteria material and sewage treatment method

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preparation example Construction

[0035] two. Preparation of magnetic iron oxide nanomaterials:

[0036] The magnetic iron oxide nanoparticles described in the present invention are self-made by our laboratory, and the method is a chemical co-precipitation method, and the particle size of the obtained nano iron oxide is about 10-20 nm. Concrete preparation steps are as follows:

[0037] According to Fe 3+ and Fe 2+ Feed at a molar ratio of 2:1, and dissolve 54.05 g of FeCl with 200 ml of 2M hydrochloric acid solution with ultrasonic stirring 3 ·6H 2 O, added to a 3 L single-layer glass reactor, stirred with nitrogen gas. Dissolve 27.80g of FeSO with 50 ml of 2M hydrochloric acid solution under ultrasonic conditions 4 ·7H 2 O, then add the above Fe 3+ in solution. Under the condition of stirring with nitrogen gas, 1.25 L of 12.5% ​​(solvent accounted for the mass fraction of the solution) (CH 3 ) 4 The NOH aqueous solution was quickly added to the iron salt solution, and reacted at room temperature ...

Embodiment 1

[0048] 1. By chemical co-precipitation method, nano-iron oxide particles with a particle size of about 10nm ( figure 1 ).

[0049] The biochar material is prepared by anaerobic pyrolysis method, which is a porous carbon material ( figure 2 ).

[0050] By means of self-assembly, the nano-iron oxide particles are loaded on the surface of the biochar material to obtain a magnetic composite bio-char material ( image 3 ).

[0051] By co-incubation is the method, the photosynthetic bacteria R. palustris Loaded on the surface of magnetic biochar, the adsorption of microorganisms by magnetic biochar is very fast, and the loading capacity is 5.45×10 within 2 hours 9 (cfu / g), load rate 90.8%. ( Figure 4 )

[0052] The specific process is: Rhodopseudomonas palustris R. palustris Expand training:

[0053] (1) preparation R. palustris Solid medium (g / L): NH 4 Cl 1.2, Na 2 HPO 4 0.5, MgCl 2 0.2, NaCl 2, beef extract 2, organic acid 2, agar powder 12. The pH was adjusted ...

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Abstract

The invention relates to the technical field of sewage treatment, and in particular to preparation method of magnetic biochar loaded photosynthetic bacteria material and a sewage treatment method. The preparation method comprises the steps of: preparing a magnetic nano iron oxide material by a chemical coprecipitation method, conducting activation and expansion culture on photosynthetic bacteria, loading the nano iron oxide particles on the surface of biological carbon material through a self-assembly method to form a magnetic biochar; and loading the photosynthetic bacteria on the surface of the magnetic biochar through a co-incubation method, so as to obtain the magnetic biochar loaded photosynthetic bacteria material. The magnetic biochar loaded photosynthetic bacteria material is co-incubated with sewage for a period of time, so as to realize degradation of COD, ammonia nitrogen and phosphate radical in the sewage. The invention employs the electrostatic binding of high specific surface of the nano magnetic iron oxide and the bacteria with charge on the surface to achieve immobilization of strains, and magnetic iron oxide material has activation on strain enzyme, thereby enhancing the degradation of COD, ammonia nitrogen and phosphate radical by microbes.

Description

technical field [0001] The invention relates to the technical field of sewage treatment, in particular to a technology for coupling nanomaterials and microorganisms, which is applied to sewage treatment and can quickly and effectively degrade COD, ammoniacal nitrogen and phosphate radicals. Background technique [0002] With the rapid development of industry and agriculture and the increasing discharge of domestic sewage, global water pollution is becoming more and more serious, threatening the sustainable development of social economy and even human beings. Therefore, it is necessary to develop advanced sewage treatment technology with strong stability, high efficiency and simple operation. Organic matter, nitrogen and phosphorus are the main pollution sources of water bodies. At present, the main treatment methods include adsorption method, photocatalytic oxidation method, chemical precipitation method and biological method. These methods have their own advantages and dis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34
CPCC02F3/34
Inventor 何世颖俞映倞冯彦房段婧婧薛利红杨林章
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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